Interaction of the human intestinal microbiota with the release of bound phenolic compounds in chickpea ( Cicer arietinum L.)

2021 ◽  
Author(s):  
Liliana Maribel Perez‐Perez ◽  
José Ángel Huerta‐Ocampo ◽  
José Rogelio Ramos‐Enríquez ◽  
Saúl Ruiz‐Cruz ◽  
Francisco Javier Wong‐Corral ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Cicer Arietinum ◽  
Intestinal Microbiota ◽  
Cicer Arietinum L ◽  
Human Intestinal Microbiota

Profiling of phenolic and other compounds from Egyptian cultivars of chickpea (Cicer arietinum L.) and antioxidant activity: a comparative study

RSC Advances ◽  
2015 ◽  
Vol 5 (23) ◽  
pp. 17751-17767 ◽  
Author(s):  
Reham Hassan Mekky ◽  
María del Mar Contreras ◽  
Mohamed Roshdi El-Gindi ◽  
Azza R. Abdel-Monem ◽  
Essam Abdel-Sattar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Comparative Study ◽  
Cicer Arietinum ◽  
Metabolic Profiling ◽  
Cicer Arietinum L ◽  
Rp Hplc

The metabolic profiling of seven Egyptian cultivars of chickpeaviaRP-HPLC-DAD-ESI-QTOF-MS enabled the characterization of 96 phenolic compounds.

Release of Small Phenolic Compounds from Brewer’s Spent Grain and Its Lignin Fractions by Human Intestinal Microbiota in Vitro

2013 ◽  
Vol 61 (40) ◽  
pp. 9744-9753 ◽  
Author(s):  
Anna-Marja Aura ◽  
Piritta Niemi ◽  
Ismo Mattila ◽  
Klaus Niemelä ◽  
Annika Smeds ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Microbiota ◽  
Brewer’S Spent Grain ◽  
Human Intestinal Microbiota ◽  
Spent Grain

Accumulation of Phenolic Compounds and Phytoalexins in Sliced and Elicitor-Treated Cotyledons of Cicer arietinum L.

1987 ◽  
Vol 42 (11-12) ◽  
pp. 1171-1178 ◽  
Author(s):  
Ulrike Jaques ◽  
Helmut Keßmann ◽  
Wolfgang Barz
Keyword(s):  
Phenolic Compounds ◽  
Cicer Arietinum ◽  
Structural Elucidation ◽  
Ascochyta Rabiei ◽  
Spectroscopic Techniques ◽  
Chromatographic Purification ◽  
Cicer Arietinum L ◽  
Phytoalexin Biosynthesis

Upon slicing cotyledons of chickpea, Cicer arietinum L.. accumulate the pterocarpan phyto­alexins medicarpin and maackiain. Treatment of this tissue with an elicitor from the phytopathogcnic deuteromyccte Ascochyta rabiei (Pass.) Lab. greatly enhances accumulation of the pterocarpans and of other isoflavones and flavonoids. Isolation, chromatographic purification and structural elucidation by spectroscopic techniques of 16 phenolic compounds is described. Cotyledons induced for phytoalexin biosynthesis readily accumulate the isoflavones daidzein, formononetin, calycosin and pseudobaptigenin which are thought to be intermediates in pterocarpan formation.

DIFERENCIACIÓN DE ACCESIONES DE GARBANZO MEDIANTE CARACTERES MORFOLÓGICOS Y MOLECULARES

2018 ◽  
Vol 41 (4) ◽  
pp. 469-476
Author(s):  
Ernestina Valadez-Moctezuma ◽  
Anselmo de J. Cabrera-Hidalgo
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L

El garbanzo (Cicer arietinum L.) es una de las principales leguminosas de grano cultivadas en el mundo. México es de los diez principales productores a nivel mundial con una producción total de 171 mil toneladas de grano. Este cultivo presenta alto nivel de autogamia y de monotonía genética, lo que dificulta la diferenciación de genotipos élite. En este estudio preliminar se evaluó la variabilidad de 57 genotipos de diferentes áreas geográficas mediante caracteres morfológicos y marcadores moleculares tipo RAPD e ISSR. El análisis de correspondencia múltiple mostró que los mayores valores discriminantes fueron el color de la flor azul y semillas grandes y de forma redondeada, pero el agrupamiento respectivo no diferenció a las accesiones, incluyendo las especies silvestres; sin embargo, el análisis UPGMA logró una mejor separación. Los marcadores RAPD aun cuando generaron perfiles de ADN, no fueron informativos, mientras que los ISSR diferenciaron a las 57 accesiones de C. arietinum utilizadas y a la especie silvestre C. reticulatum, lo que los hace buenos candidatos para caracterizar este cultivo. Este estudio sirvió como base para desarrollar otro sistema de marcadores moleculares más eficiente en esta especie.

scholarly journals Discovery of novel community-relevant small proteins in a simplified human intestinal microbiome

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Hannes Petruschke ◽  
Christian Schori ◽  
Sebastian Canzler ◽  
Sarah Riesbeck ◽  
Anja Poehlein ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Intestinal Microbiota ◽  
De Novo ◽  
Bacterial Species ◽  
Intestinal Microbiome ◽  
Single Strain ◽  
Small Proteins ◽  
Human Intestinal Microbiota ◽  
Wide Range

Abstract Background The intestinal microbiota plays a crucial role in protecting the host from pathogenic microbes, modulating immunity and regulating metabolic processes. We studied the simplified human intestinal microbiota (SIHUMIx) consisting of eight bacterial species with a particular focus on the discovery of novel small proteins with less than 100 amino acids (= sProteins), some of which may contribute to shape the simplified human intestinal microbiota. Although sProteins carry out a wide range of important functions, they are still often missed in genome annotations, and little is known about their structure and function in individual microbes and especially in microbial communities. Results We created a multi-species integrated proteogenomics search database (iPtgxDB) to enable a comprehensive identification of novel sProteins. Six of the eight SIHUMIx species, for which no complete genomes were available, were sequenced and de novo assembled. Several proteomics approaches including two earlier optimized sProtein enrichment strategies were applied to specifically increase the chances for novel sProtein discovery. The search of tandem mass spectrometry (MS/MS) data against the multi-species iPtgxDB enabled the identification of 31 novel sProteins, of which the expression of 30 was supported by metatranscriptomics data. Using synthetic peptides, we were able to validate the expression of 25 novel sProteins. The comparison of sProtein expression in each single strain versus a multi-species community cultivation showed that six of these sProteins were only identified in the SIHUMIx community indicating a potentially important role of sProteins in the organization of microbial communities. Two of these novel sProteins have a potential antimicrobial function. Metabolic modelling revealed that a third sProtein is located in a genomic region encoding several enzymes relevant for the community metabolism within SIHUMIx. Conclusions We outline an integrated experimental and bioinformatics workflow for the discovery of novel sProteins in a simplified intestinal model system that can be generically applied to other microbial communities. The further analysis of novel sProteins uniquely expressed in the SIHUMIx multi-species community is expected to enable new insights into the role of sProteins on the functionality of bacterial communities such as those of the human intestinal tract.

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