Digestion of pectic polysaccharide from Brassica rapa L. in vitro and its effect on the intestinal microbiota in cyclophosphamide‐treated mice

2021 ◽  
Author(s):  
Wei Wang ◽  
Lijun Fan ◽  
Xuewen Li ◽  
Zirong Wang ◽  
Jianzhong Zhou ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Intestinal Microbiota ◽  
Pectic Polysaccharide

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

scholarly journals Inside Front Cover: Species Differences in in vitro and Estimated in vivo Kinetics for Intestinal Microbiota Mediated Metabolism of Acetyl‐deoxynivalenols

2021 ◽  
Vol 65 (9) ◽  
pp. 2170020
Author(s):  
Jing Jin ◽  
Albertus Spenkelink ◽  
Karsten Beekmann ◽  
Marta Baccaro ◽  
Fuguo Xing ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Species Differences ◽  
Front Cover ◽  
In Vivo Kinetics

Establishment of an efficient shoot regeneration system in vitro in Brassica rapa

2021 ◽  
Author(s):  
Ying Zhao ◽  
Shengnan Huang ◽  
Yun Zhang ◽  
Fengyan Shi ◽  
Xuyao Liu ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Shoot Regeneration ◽  
Regeneration System

A pectic polysaccharide from peach palm fruits (Bactris gasipaes) and its fermentation profile by the human gut microbiota in vitro

2017 ◽  
Vol 9 ◽  
pp. 1-6 ◽  
Author(s):  
Thaisa Moro Cantu-Jungles ◽  
Thales R. Cipriani ◽  
Marcello Iacomini ◽  
Bruce R. Hamaker ◽  
Lucimara M.C. Cordeiro
Keyword(s):  
Gut Microbiota ◽  
Bactris Gasipaes ◽  
Human Gut ◽  
Pectic Polysaccharide ◽  
Peach Palm ◽  
Human Gut Microbiota ◽  
Fermentation Profile ◽  
Palm Fruits

scholarly journals In vitro fermentation of arabinoxylan from oat (Avena sativa L.) by Pekin duck intestinal microbiota

3 Biotech ◽  
2019 ◽  
Vol 9 (2) ◽  
Author(s):  
Dandan Tian ◽  
Xiaoqing Xu ◽  
Qing Peng ◽  
Zhiguo Wen ◽  
Yuwei Zhang ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Avena Sativa ◽  
Pekin Duck ◽  
In Vitro Fermentation

scholarly journals Phytoestrogens: Dietary Intake, Bioavailability, and Protective Mechanisms against Colorectal Neoproliferative Lesions

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1709 ◽  
Author(s):  
Maria Teresa Viggiani ◽  
Lorenzo Polimeno ◽  
Alfredo Di Leo ◽  
Michele Barone
Keyword(s):  
Dietary Intake ◽  
Intestinal Microbiota ◽  
Estrogen Receptor Beta ◽  
Epidemiological Data ◽  
In Vivo Studies ◽  
Beneficial Effects ◽  
Natural Substances

Phytoestrogens are natural substances that have been extensively studied for their beneficial effect on human health. Herein, we analyzed the data of the literature on the role of phytoestrogens in the prevention of colorectal neoproliferative lesions (CNL). Both in vitro and in vivo studies suggest that the beneficial effects of phytoestrogens on CNL mainly depend on their ability to bind estrogen receptor beta (ERβ) in the intestinal mucosa and counter ER-alpha (ERα) activity. Epidemiological data demonstrate a correlation between the low prevalence of CNL in Eastern populations and the consumption of soy products (phytoestrogen-enriched diet). However, both observational and interventional studies have produced inconclusive results. In our opinion, these discrepancies depend on an inadequate evaluation of phytoestrogen intake (dietary questionnaires were not aimed at establishing phytoestrogen intake) and absorption (depending mainly on the intestinal microbiota of the analyzed subjects). For this reason, in the present review, we performed an overview of phytoestrogen dietary intake and metabolism to offer the reader the opportunity for a better interpretation of the literature. Future prospective trials focusing on the protective effect of phytoestrogens against CNL should take into account both their dietary intake and absorption, considering the effective role of the intestinal microbiota.

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