Discrimination of Kyoho grape ( Vitis labruscana ) skin, seed, and flesh antioxidant activities by solvent extraction: Application of advanced chemometrics

Author(s):  
Kandi Sridhar ◽  
Albert Linton Charles
2013 ◽  
Vol 834-836 ◽  
pp. 577-581
Author(s):  
Jian Chao Deng ◽  
Gai Gai Niu ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Yong Chuan Deng ◽  
...  

Flavonoids was isolated from canavalia maritime by accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC). The antioxidant activities of flavonoids were investigated in vitro and evaluated by IC50. The results indicated that flavonoids possessed obvious reducing power and significant inhibitory effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggest that canavalia maritime flavonoids could be a suitable natural antioxidant for humans.


1979 ◽  
Vol 52 (5) ◽  
pp. 949-961 ◽  
Author(s):  
G. Scott ◽  
K. V. Smith

Abstract The antioxidant activities of a number of N-phenyl nitrones containing phenolic functions were compared with N-methyl nitrones and conventional antioxidants in peroxide and TMTD sulphurless vulcanizates. The N-phenyl nitrones were found to be effectively bound during vulcanization; whereas, the N-methyl nitrones were not. Unhindered phenols reacted to a higher degree and were relatively more effective than the typical hindered phenol antioxidant structures. A conventional bisphenol was effective in the sulphurless vulcanizate after solvent extraction, but this is considered to be due to the formation of an insoluble zinc salt. Synergism was observed in the TMTD sulphurless vulcanizate both before and after extraction.


Author(s):  
Towkir Ahmed Ove ◽  
Anwara Akter Khatun ◽  
Said Bin Saifullah ◽  
Maruf Ahmed

Aims: This study was intended to investigate and quantify the phytochemicals and the antioxidant activities of fresh and powdered wheatgrass. Study Design:  The experiment was performed by solvent extraction technique for determining the phytochemicals of wheatgrass samples. Place and Duration of Study: The study was conducted at the Food engineering laboratory in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh between June 2018 and December 2018. Methodology: Fresh and powdered wheatgrass were used for the experiment. Methanol and ethanol solutions were separately utilized, in an attempt to assume the efficient solvent for extraction. UV-Vis spectrophotometric methods were employed in general throughout the study. Total phenolic contents (TPC), total flavonoid contents (TFC), DPPH scavenging activity, ferric reducing antioxidant power (FRAP) and the total chlorophyll contents of both solvent extracts were analyzed. Results: Phytochemicals and antioxidant activity were found lower in both solvent extracts of powdered wheatgrass than fresh leaves. Phenolic content and Chlorophyll were significantly (P < 0.05) higher in fresh samples (extracts). Due to heating, a significant decrease was observed in the DPPH radical scavenging activity of powdered extracts. Ferric Reducing Antioxidant Power (FRAP) was significantly (P < 0.05) higher in methanolic extracts than in ethanolic extracts. No significant (P > 0.05) difference was observed in total flavonoids contents. Conclusion: These findings endorsed that both solvents demonstrate competitive potentials to extract bioactive compounds from wheatgrass and its derivatives and hence can be chosen specifically according to the components.


2018 ◽  
Vol 12 (1) ◽  
pp. 9-20
Author(s):  
Agus Sudibyo ◽  
Tiurlan Farida Hutajulu ◽  
Maman Sukiman

Study on the preparation proces of curcuminoid from turmeric root (Curcuma longa domestica Vahl) and its characteristics as food ingredient was conducted. The results revealed that the type of solvent, the solid-liquid ratio and extraction temperature were affected to the yield of curcuminoid content. The yield of curcuminoid content ranged from 11.96% to 14.01% (for ethanolic 60% solvent extraction), ranged from 12.91% to 15.20% (ethanolic 80% solvent extraction), and ranged from 3.85% to 7.19% (ethanolic : water 1 : 1  extraction). Results showed that the highest value of total phenolic content was recorded in ethanolic extract (S/L ratio 1 : 50 and extraction temperature 60oC) with value 146.25 mg GAE/100 g; while the lowest for a mixture of ethanol and water 1 : 1 extract (S/L ratio 1 : 30) and extraction temperature 30oC with value 101.43 mg GAE/100 g.  Results also showed that the highest  value antioxidant activities was recorded in ethanolic 80% extract and 15.00 g ground dry turmeric used with value 47.65% ; while the lowest  value from a mixture of ethanolic and water 1 : 1  extract and 1.50 g ground dry turmeric used with value 20.04%.                                                                     ABSTRAK Studi pada proses pembuatan bubuk curcuminoid dari akar kunyit (Curcuma longa domestica Vahl dan karakteristiknya sebagai bahan pangan telah dilakukan. Hasil percobaan menunjukkan bahwa jenis pelarut, perbandingan antara padatan - larutan dan suhu ekstrasi yang digunakan berpengaruh terhadap rendemen kandungan curcuminoid. Kandungan curcumnoid berkisar antara 11,96% hingga 14,01% (untuk ekstrasi dengan pelarut ethanol 60%), berkisar antara 12,91% hingga 15,20% (ekstrasi dengan pelarut ethanol 80%), dan berkisar antara 3,85 hingga 7,19% (ekstrasi dengan pelarut ethanol 50 %). Hasil penelitian menunjukkan bahwa nilai ter tinggi dan terbaik kandungan total senyawa fenol tercatat pada ekstrasi dengan ethanol (perbandingan S/L 1 : 50 dan suhu ekstrasi 60o C)  dengan nilai 148,99 mg GAE/100 g sedang nilai terendah tercatat untuk ekstrasi menggunakan pelarut 50% (perbandingan S/L 1 : 30) dan suhu ekstrasi 30 o C dengan nilai 101,43 mg GAE/100 g. Hasil penelitian juga menunjukkan bahwa nilai  tertinggi aktifitas antioksidan tercatat pada ekstraksi menggunakan ethanol 80% dan menggunakan bahan kunyit kerin g sebanyak 15,0 g dengan nilai 47,65% ; sedang nilai terendah sebagai aktifitas antioksidan pada ekstrasi menggunakan ethanol 50% dan menggunakan bahan kunyit kering sebanyak 1,50 g dengan nilai 20,04% . Kata kunci : Rimpang kunyit (Curcuma longa domestica Vahl), kurkuminoid, proses ekstrasi, sifat karakteristik, ingredien pangan . 


2020 ◽  
Vol 01 ◽  
Author(s):  
Mariza Vaso ◽  
Wesam Ali ◽  
Muhammad Irfan Masood ◽  
Muhammad Jawad Nasim ◽  
Rainer Lilischkis ◽  
...  

Background: Nigella sativa seeds, commonly referred to as “black cumin”, are rich in a spectrum of biologically active substances and thus associated with a range of potential health benefits. Unlocking the activity of these phytochemicals traditionally requires solvent extraction. Previously, we have explored nanosizing as alternative to liberate biological activity of natural products, such as Solanum incanum, Pterocarpus erinaceus, Loranthus micranthus and Cynomorium coccineum. Objectives: Nanosizing natural products may increase their activity for a number of reasons ranging from an improved bioavailability to physical nano-toxicity. Because Nigella sativa is amenable to being “milled down”, this spice has been employed to explore the underlying causes of increased activity upon mechanical particle size reduction. Methods: Nigella sativa seeds were pre-milled employing a household flour mill followed by extensive grinding exploiting a planetary ball mill. The particles were characterized employing Laser Diffraction, Photon Correlation Spectroscopy and Scanning Electron Microscopy connected with Energy Dispersive X-ray Diffraction. Finally, the samples were evaluated for sterility profile, nematicidal and antioxidant activities. Results: Suspensions containing fairly uniform particles of Nigella sativa showing diameters in the range of 100-1000 nm have been obtained. These nanosuspensions are characterized by a considerably reduced microbial contamination when compared to the bulk material and reasonable antioxidant and nematicidal activity when employed in higher concentrations. This activity is comparable to the ethanolic extract of the seeds and is significantly higher when compared to aqueous extracts. A combination of “sterilization and extraction by milling”, improved liberation of soluble substances from small particles, surface activity and physical nano-activity of the particles itself is likely responsible for the activities observed. Conclusion: Nanosizing of entire natural products provides an interesting alternative to solvent extraction as it results in the liberation of active ingredients and certain additional activities. The resulting nanosuspensions can be investigated further and optimized for applications in Nutrition, Agriculture, Medicine and Cosmetics.


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