Establishment of a method for the simultaneous detection of four foodborne pathogens using high‐throughput suspension arrayxTAGtechnology

2019 ◽  
Vol 54 (8) ◽  
pp. 2578-2585
Author(s):  
Yunxue Shi ◽  
Yan Wang ◽  
Yufei Tian ◽  
Wensen Liu ◽  
Wenhe Zhu ◽  
...  
Keyword(s):  
High Throughput ◽  
Foodborne Pathogens ◽  
Simultaneous Detection

scholarly journals High Throughput Sequencing for Detection of Foodborne Pathogens

2017 ◽  
Vol 8 ◽  
Author(s):  
Camilla Sekse ◽  
Arne Holst-Jensen ◽  
Ulrich Dobrindt ◽  
Gro S. Johannessen ◽  
Weihua Li ◽  
...  
Keyword(s):  
High Throughput ◽  
Foodborne Pathogens ◽  
High Throughput Sequencing

scholarly journals A new high-throughput method for simultaneous detection of drug resistance associated mutations in Plasmodium vivax dhfr, dhps and mdr1 genes

2011 ◽  
Vol 10 (1) ◽  
pp. 282 ◽  
Author(s):  
Céline Barnadas ◽  
David Kent ◽  
Lincoln Timinao ◽  
Jonah Iga ◽  
Laurie R Gray ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Vivax ◽  
High Throughput ◽  
Simultaneous Detection ◽  
High Throughput Method

scholarly journals SERS-based droplet microfluidics for high-throughput gradient analysis

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 674-681 ◽  
Author(s):  
Jinhyeok Jeon ◽  
Namhyun Choi ◽  
Hao Chen ◽  
Joung-Il Moon ◽  
Lingxin Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Gradient Analysis ◽  
Simultaneous Detection ◽  
Droplet Microfluidics ◽  
Fully Integrated

We report a fully integrated SERS-based microdroplet platform for simultaneous detection of various concentrations of a reagent.

Developing a multiplex real-time PCR with a new pre-enrichment to simultaneously detect four foodborne bacteria in milk

2019 ◽  
Vol 14 (10) ◽  
pp. 885-898 ◽  
Author(s):  
Moezi Parichehr ◽  
Kargar Mohammad ◽  
Doosti Abbas ◽  
Khoshneviszadeh Mehdi
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reliable Technique ◽  
E Coli ◽  
Foodborne Bacteria ◽  
Multiple Pathogens ◽  
Enrichment Step

Aim: The aim of this study is to formulate a new single nonselective pre-enrichment medium (ELSS) that can support the concurrent growth of four major foodborne pathogens containing E. coli O157: H7, L. monocytogenes, S. aureus and S. enterica serovar Entertidis to develop a multiplex TaqMan Real-time PCR (mRT-PCR). Methods: The mRT-PCR with a new pre-enrichment was carried out for simultaneous detection and quantification of these foodborne bacteria. Results: By using mRT-PCR after 16 h pre-enrichment in ELSS, the detection limit of each pathogen was 1 CFU/25 ml contaminated milk, as well as inclusivity and exclusivity reached 100%. Conclusion: The mRT-PCR assay with pre-enrichment step is a fast and reliable technique for detecting single or multiple pathogens in food products.

Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1160-1165 ◽  
Author(s):  
Pu Su ◽  
Nan Liu ◽  
Maoxiang Zhu ◽  
Baoan Ning ◽  
Ming Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Simultaneous Detection ◽  
Suspension Array ◽  
Array Technology

scholarly journals Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Sonja U. Weishaupt ◽  
Steffen Rupp ◽  
Karin Lemuth
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Human Cancer ◽  
Simultaneous Detection ◽  
Human Tumor ◽  
Mirna Sequence ◽  
Single Experiment ◽  
Detection Range ◽  
Precursor Mirnas ◽  
Zip Code

MicroRNAs (miRNAs) are important negative regulators of gene expression. Their implication in tumorigenesis is based on their dysregulation in many human cancer diseases. Interestingly, in tumor cells, an altered ratio of precursor and mature miRNA levels has been described. Consequently, differences in miRNA type levels have a high potential as biomarkers and comparative high-throughput-based detection might permit a more accurate characterization of subtypes, especially in the case of very heterogeneous tumor entities. Several molecular methods exist for the detection of mature and precursor miRNAs. DNA microarrays are predestinated as a high-throughput method for comprehensive miRNA detection in tumors. However, the simultaneous array-based detection of both these miRNA types is limited because the mature miRNA sequence is identically present in both forms. Here we present a ZIP-code DNA microarray-based system in combination with a novel labeling approach, which enables the simultaneous detection of precursor and mature miRNAs in one single experiment. Using synthetic miRNA templates, we demonstrate the specificity of the method for the different miRNA types, as well as the detection range up to four orders of magnitude. Moreover, mature and precursor miRNAs were detected and validated in human tumor cells.

Simultaneous detection of multiple viruses based on chemiluminescence and magnetic separation

2017 ◽  
Vol 5 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Zeeshan Ali ◽  
Jiuhai Wang ◽  
Yongjun Tang ◽  
Bin Liu ◽  
Nongyue He ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Throughput ◽  
Dna Hybridization ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Reliable Detection ◽  
Automated Platform ◽  
Modified Magnetic Nanoparticles ◽  
Surface Modified ◽  
Multiple Pathogens

In this report, a DNA hybridization based chemiluminescent detection method has been proposed for reliable detection of multiple pathogens. The use of surface modified magnetic nanoparticles can help to integrate this system into an automated platform for high throughput applications.

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