Effect of commercial enzymatic preparation with pectolytic activities on conventional extraction and ultrasound-assisted extraction of oil from grape seed (Vitis viniferaL.)

Author(s):  
Carla Da Porto ◽  
Deborha Decorti ◽  
Andrea Natolino
2021 ◽  
Vol 43 ◽  
pp. e55564
Author(s):  
Suelen Siqueira dos Santos ◽  
Carolina Moser Paraíso ◽  
Letícia Misturini Rodrigues ◽  
Grasiele Scaramal Madrona

Blueberry and raspberry pomace are a rich source of bioactive compounds that have not been commercially utilized yet, and ultrasound-assisted technology can efficiently extract these compounds. Also, the use of water as a solvent added to the ultrasound-assisted technology improves this eco-friendly process. Therefore, an aqueous eco-friendly extraction, including extraction time and ultrasound presence or absence (conventional extraction) was performed in order to extract bioactive compounds from blueberry and raspberry pomace. Response parameters included levels of anthocyanins, phenolic compounds, and flavonoids, and antioxidant activity determined by DPPH, ABTS, and FRAP methods. Analysis of variance results indicated that ultrasound-assisted extraction for 45 min. was feasible to extract the bioactive compounds. The antioxidant content of the extract obtained by the ultrasound-assisted process was 1.4 times higher on average and the total phenolic concentration was 1.6 times higher (for blueberry 5.02 and for raspberry 2.53 mg gallic acid equivalent/g) compared with those obtained by the conventional process. Thus, the ultrasound-assisted extraction method can be a profitable alternative to extract bioactive compounds from blueberry and raspberry pomace, as it is energy efficient, requires fewer chemicals, and produces less effluent. This eco-friendly technology is therefore viable for food, nutraceutical, and cosmetic industries, and also for reducing food waste.


Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1718 ◽  
Author(s):  
Cristina Alcántara ◽  
Tihana Žugčić ◽  
Radhia Abdelkebir ◽  
Jose V. García-Pérez ◽  
Anet Režek Jambrak ◽  
...  

Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products.


2018 ◽  
Vol 98 (13) ◽  
pp. 5019-5026 ◽  
Author(s):  
Bruna R Böger ◽  
Aroldo Salviato ◽  
Daniel F Valezi ◽  
Eduardo Di Mauro ◽  
Sandra R Georgetti ◽  
...  

2020 ◽  
Vol 14 (4) ◽  
Author(s):  
Vasfiye-Hazal Ozyurt ◽  
Semih Otles

Dietary fibre and polyphenols have been widely used to increase the functionality of some foods because of their potential  effects on human health. In this study, extraction of dietary fibre and polyphenols from pomace obtained as a by-product of processing carob molasses has been studied. The dietary fibre and polyphenol extracts were prepared separately. The amount of dietary fibre in the carob molasses pomace was evaluated with two assays: the Association of Official  Analytical Chemists’ enzymatic-gravimetric method and the enzymatic-chemical method. The methods were compared, each having been preceded by conventional extraction and ultrasound-assisted extraction of carob  molasses pomace. It has been found that when the enzymatic-gravimetric method and the ultrasound-assisted extraction method were used, the total dietary fibre contents were significantly higher than after using the enzymatic-chemical method and the conventional extraction method. Conventional extraction of polyphenols from carob molasses pomace has shown a relatively higher polyphenol content and antioxidant activity than ultrasound-assisted extraction. Ultrasound-assisted extraction  took  less time than conventional extraction did. The polyphenol profile was characterised by means of a high-performance liquid-chromatography diode array detector using 10 phenolic standards. Six compounds, i.e. caffeic, syringic, -epicatechin, trans-cinnamic  acid,  myricetin,  and naringin were determined with the high-performance liquid chromatography-diode array detector using both conventional extraction and the ultrasound-assisted extraction. Our results suggest that carob molasses pomace can be used to prepare low-calorie, high-fibre, and antioxidant-rich foods, nutraceuticals, and pharmaceuticals.


Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 904 ◽  
Author(s):  
Gabriela Aguilar-Hernández ◽  
María García-Magaña ◽  
María Vivar-Vera ◽  
Sonia Sáyago-Ayerdi ◽  
Jorge Sánchez-Burgos ◽  
...  

Ultrasound-assisted extraction (UAE) is widely used; however, the efficiency of extraction depends on the raw materials. Therefore, optimization of UAE must be investigated for each type of plant material. By-products from soursop fruit have not been studied as a source of bioactive compounds. In this work, the optimization of UAE conditions (extraction time (5, 10, and 15 min), pulse cycle (0.4, 0.7, and 1 s), and sonication amplitude (40%, 70%, and 100%)) for the extraction of phenolic compounds (soluble, hydrolyzable, condensed tannins, and total polyphenols) from soursop by-products (seed, peel, and columella) and pulp was evaluated using response surface methodology. The optimal conditions for UAE to obtain the highest total polyphenol content from by-products and pulp was dependent on the raw material. Peel resulted in the highest content of total polyphenols (187.32 mg/g dry matter [DM]) followed by columella (164.14 mg/g DM), seed (36.15 mg/g DM), and pulp (33.24 mg/g DM). The yield of polyphenolic content from peel and columella obtained with UAE was higher (32–37%) than conventional extraction for 2 h under stirring (14–16%). The contents of gallic acid (0.36–15.86 µg/g DM), coumaric acid (0.07–1.37 µg/g DM), and chlorogenic acid (9.18–32.67 µg/g DM) in the different parts of the fruit were higher in the extracts obtained by UAE compared with a conventional extraction method (0.08–0.61, 0.05–0.08, 3.15–13.08 µg/g DM, respectively), although it was dependent on the raw materials. Soursop by-products can be functionally important if they are used to extract bioactive compounds by UAE; a technology with high potential for commercial extraction on a large scale.


2019 ◽  
Vol 891 ◽  
pp. 83-89
Author(s):  
Attapon Nitiwattananon ◽  
Saipin Thanachasai

In this study, ultrasound-assisted extraction (UAE) was compared with conventional extraction methods, including conventional solvent extraction without agitation (CSE), conventional solvent extraction with agitation at 50 rpm (CSE50) and 150 rpm (CSE150), for the extraction of phenolic compounds from coconut (Cocos nucifera L.) husk. The extraction yield, total phenolic content (TPC) and total flavonoid content (TFC) were examined. The antioxidant capacity of C. nucifera extracts was determined by using 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Experimental results showed that UAE gave the highest extraction yield, TPC, TFC and antioxidant capacities (ABTS and DPPH), followed by CSE150, CSE50 and CSE, respectively. UAE was found to be more effective than conventional extraction methods. Conventional solvent extraction with higher agitation speed exhibited higher extraction efficiency than those with lower agitation speed and without agitation.


Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1864
Author(s):  
Jelena Milanović ◽  
Marko Malićanin ◽  
Vesna Rakić ◽  
Nenad Jevremović ◽  
Ivana Karabegović ◽  
...  

Valorization of winery waste is a promising solution for waste management with additional benefit of bioactive compounds isolation. The aim of this study was to analyze the nutritional and bioactive potential of oil derived from Serbian autochthonous grape seed variety Prokupac and to compare it with known international grape varieties. Additionally, two extraction methods (ultrasound assisted extraction and cold pressing) were used in order to determine their influence on physico-chemical and nutritive characteristics of grape seed oil. Different methods, including determination of physicochemical properties, content of bioactive compounds, oxidative stability and antioxidative capacity were used for the characterization of grape seed oils. Results indicated that the higher yield was obtained by ultrasound assisted extraction compared to cold pressing. The application of ultrasound assisted extraction resulted in increased α-tocopherol content and better antioxidant capacity and oxidative stability of oil. Significant differences in bioactive composition were observed for Prokupac seed oil compared to the other grape varieties. Oil extracted from Prokupac grape seed had the lowest content of polyunsaturated and the highest content of saturated fatty acids, the highest content of α-tocopherol and consequently, the best antioxidative capacity. Prokupac grape seed has a great potential for isolation of nutritive and bioactive valuable oil.


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