scholarly journals Comparison of Rapid Antibody Test and Thorax Computed Tomography Results in Patients who Underwent RT‐PCR with the Pre‐Diagnosis of COVID‐19

2021 ◽  
Author(s):  
Ilker Kızıloglu ◽  
Aslı Sener ◽  
Neslihan Siliv
Keyword(s):  
Computed Tomography ◽  
Antibody Test ◽  
Rt Pcr ◽  
Rapid Antibody

scholarly journals Comparison of Rapid Antibody Test and Thorax Computed Tomography Results in Patients who Underwent RT-PCR with the Pre-Diagnosis of COVID-19

2021 ◽  
Author(s):  
İlker Kızıloglu ◽  
Aslı Şener ◽  
Neslihan Siliv
Keyword(s):  
Computed Tomography ◽  
Emergency Department ◽  
Predictive Value ◽  
Antibody Test ◽  
Nasopharyngeal Swab ◽  
Test Results ◽  
Rt Pcr ◽  
Hospital Data ◽  
Thorax Ct ◽  
Rapid Antibody

Introduction: In this study, it is planned to compare the RT-PCR test, which is the gold standard in the diagnosis of COVID-19, with Thorax computed tomography (CT) and rapid antibody test results. Methods: Patients who were admitted to the emergency service of İzmir Çiğli Training and Research Hospital between 01.04.2020 and 31.05.2020 and who were suspected of having COVID-19 infection were included in the study. The medical records of the patients were retrospectively analyzed through the hospital data processing database. Age, gender, hospitalization, status of home quarantine, real-time reverse transcription-polymerase chain reaction (RT-PCR), thorax CT and rapid antibody test results of the patients were examined. The relationship between RT-PCR, thorax CT and rapid antibody test results were compared statistically. Results: A total of 181 patients, 115 (63.5%) male and 66 (36.5%) female, with an average age of 56.4 ± 18.06 years were included in the study. The nasopharyngeal swab PCR result obtained at the first admission of the patients to the emergency department was positive in 71 (39.2%) patients. Thorax CT was performed in 173 (95.6%) patients who applied to the emergency department, and 112 (64.7%) of them had findings that could be compatible with COVID-19. According to the thorax CT findings in patients, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for detecting COVID-19 infection were respectively; 76.1%, 43.1%, 48.2% and 72.1% (ĸ: 0.176, p <0.001). In our study, the mortality rate for COVID-19 was found to be 2.8%. Conclusion: Rapid antibody test and thorax CT examinations were found to have low diagnostic value in patients who admitted to the emergency department of our hospital and whose first RT-PCR SARS-CoV-2 test was positive. Studies involving larger patient groups are needed for their use alone in diagnosis and screening.

scholarly journals Evaluation of rapid antibody test and chest computed tomography results of COVID-19 patients: A retrospective study

2021 ◽  
Author(s):  
Ali Ozturk ◽  
Taylan Bozok ◽  
Tugce Simsek Bozok
Keyword(s):  
Computed Tomography ◽  
Ct Scan ◽  
Antibody Test ◽  
Chest Ct ◽  
Rt Pcr ◽  
Serum Samples ◽  
Chest Computed Tomography ◽  
Ct Data ◽  
Chest Ct Scan ◽  
Rapid Antibody

AbstractBackgroundThe coronavirus disease 2019 (COVID-19) continues to spread around the world. Therefore, rapid, simpler, and more accurate diagnostic tests are urgently needed to diagnose the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. The purpose of this study was to evaluate the SARS-CoV-2 IgM/ IgG rapid antibody test results in symptomatic patients with COVID-19 and their chest computed tomography (CT) data.MethodsA total of 320 patients admitted to our hospital for different durations due to COVID-19 were included in the study. Serum samples were obtained within 0 to 7 days from COVID-19 patients confirmed by RT-PCR and chest CT scan. According to the SARS-CoV-2 RT-PCR results, the patients included in the study were divided into two groups: PCR positive group (n=46), and PCR negative group (n=274).ResultsOf the 320 COVID-19 serum samples, IgM, IgG, and IgM/IgG were detected in 9.4%, 3.1%, and 17.8% within one week respectively. IgG/IgM antibodies were not detected in 69.7% of the patients. In the study, it was determined that 249 (77.8%) of 320 patients had positive chest CT scans. Four (5.6%) of 100 patients with negative chest CT scan had IgM positive and 2 (2.8%) had both IgM/ IgG positive. IgM was detected in 23 (9.2%), IgG in 1 (0.4%) and IgM/IgG in 35 (14%) of chest CT scan positive patients. The rate of CT findings in patients with antibody positivity (n=97) was found to be significantly higher than those with antibody negativity.ConclusionsThe results of the present study show the accurate and equivalent performance of serological antibody assays and chest CT in detecting SARS-CoV-2 0 to 7 days from the onset of COVID19 symptoms. When the RT-PCR is not available, we believe that the combination of immunochromatographic test and chest CT scan can increase diagnostic sensitivity for COVID-19.

scholarly journals Validation of rapid antibody (IgG-IgM) test kit for SARS COV-2 infection in Qatar

2021 ◽  
Author(s):  
Jesha Mundodan ◽  
Samina Hasnain ◽  
Hayat Khogali ◽  
Soha Shawqi Al Bayat ◽  
Dina Ali ◽  
...  
Keyword(s):  
Predictive Value ◽  
Local Population ◽  
Rapid Test ◽  
Antibody Test ◽  
Molecular Testing ◽  
Rt Pcr ◽  
Test Kit ◽  
Asymptomatic Individuals ◽  
Sensitivity Specificity ◽  
Rapid Antibody

Background: In response to the growing coronavirus disease 2019 (COVID-19) pandemic and the shortage of laboratory based molecular testing capacity and reagents, multiple diagnostic test manufacturers have developed rapid and easy to use devices to facilitate testing outside laboratory settings. These kits are either based on detection of proteins from SARS-CoV-2 virus or detection of antigen or human antibodies generated in response to the infection. However, it is important to understand their performance characteristics and they must be validated in the local population setting.Design and Methods: The objective is to assess the validity of the rapid test for IgG and IgM immunoglobulins compared to the current gold standard reverse transcription polymerase chain reaction (RT-PCR) test. A total of 16951 asymptomatic individuals were tested by the Ministry of Public Health track-and-trace team using both rapid immunodiagnostic test and RT-PCR as part of screening across various random settings with potential risk of community interaction prior to gradual lifting of restrictions in Qatar.  Rapid test was considered to be posiive if both IgG and IgM are positive, while only IgG/IgM positive was considered as rapid test negative. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated.Results: The sensitivity of rapid test kit was found to be 0.9%, whereas the specificity was found to be 97.8%. the PPV was found to be 0.3% whereas the NPV was found to be 99.4%.Conclusion: Based on the outcome and results of the study, it appears that the sensitivity and PPV of the rapid antibody test are low. As such, this test is not recommended for use to assist in taking clinic-based decisions or decisions related to quarantine/isolation.

scholarly journals Potential applications of the rapid COVID-19 antibody test kit screening in comparison to the RT-PCR in patients and personnel at the Department of Obstetrics and Gynecology.

2021 ◽  
Author(s):  
Amarin Narkwichean ◽  
Wittaya Jomoui ◽  
Wipada Laosooksathit ◽  
Tanawin Nopsopon ◽  
Krit Pongpirul
Keyword(s):  
False Positive Rate ◽  
Antibody Test ◽  
Medical Personnel ◽  
Obstetrics And Gynecology ◽  
Rt Pcr ◽  
Risk Area ◽  
Asymptomatic Patients ◽  
Test Kit ◽  
Potential Applications ◽  
Rapid Antibody

Objective To explore potential applications of the rapid antibody test for COVID-19 screening, in comparison to RT-PCR, for emergency obstetric and gynecological procedures, and medical personnel in the Department of Obstetrics and Gynecology. Methods A cross-sectional study was conducted in expected 290 participants: 230 patients and 60 medical staff, during the four-month national COVID-19 outbreak period (Aug-Sep 2020, and Dec 2020-Jan 2021). All participants underwent both rapid antibody tests and RT-PCR (at admission for patients). Results A total of 270 participants completed the study. Fever and URI symptoms were present in 6/210 patients (2.8%) while one patient (0.5%) had a history of traveling to a high-risk area. However, only two (1%) asymptomatic patients had positive IgM results. Concerning the medical personnel, 10% fell into the patient under investigation (PUI) category. 4/60 (6.7%) IgM positive was observed in the staff cohort in which 3/4 came from non-PUI participants. Neither participant had RT-PCR positive demonstrating a 1.9% total false positive rate. Conclusion Rapid point-of-care antibody test can be used to screen either a pregnant coming for delivery, a patient who requires urgent/emergency operative procedures, or medical personnel, at least in the defined lower-prevalence COVID-19 situation.

scholarly journals Cohort Study: The Accuracy of Screening Methods of COVID-19 in Pregnancy: Practical Approach in Low Resources Health Services

2021 ◽  
Author(s):  
Muhammad Ilham Aldika Akbar ◽  
Khanizyah Erza Gumilar ◽  
Eccita Rahestyningtyas ◽  
Manggala Pasca Wardhana ◽  
Pungky Mulawardhana ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Pregnant Women ◽  
Antibody Test ◽  
Screening Methods ◽  
Rt Pcr ◽  
Predictive Values ◽  
X Ray ◽  
Low Middle Income Countries ◽  
Chest X Ray ◽  
Rapid Antibody

Background All pregnant women in labor should be universally screened for Coronavirus Disease 2019 (COVID-19) during pandemic periods using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) test. In many low-middle income countries, screening method was developed as an initial examination because of limited availability of RT-PCR tests. Objectives This study aims to evaluate the screening methods of COVID-19 accuracy in pregnant women. Material and Methods We recruited all pregnant women with suspicion of COVID-19 from April - August 2020 at Universitas Airlangga hospital, Surabaya, Indonesia. The participant was divided into two groups based on RT-PCR results: COVID-19 and non-COVID-19 group. The proportion of positive signs & symptoms, rapid antibody test, abnormal findings in chest x-ray, and neutrophil to lymphocyte ratio (NLR) value were then compared between both groups. The sensitivity, specificity, positive predictive value (PPV), negative predictive values (NPV), and diagnostic accuracy (DOR) were calculated. Results A total 141 pregnant women with suspected COVID-19 cases were recruited for this study. This consist of 62 COVID-19 cases (43.9%) and 79 non COVID-19 pregnant women (56.1%). The sensitivity, spesificity, PPV, NPV, and diagnostic accuracy of each parameter are as follow: clinical sign & symptoms (24.19%, 75.95%, 3.92%, 96.11%, 65.87%), rapid antibody test (72.73%, 35.06%, 4.35%, 96.94%, 36.53%), chest x-ray (40.68%, 59.45%, 3.92%, 96.11%, 58.76%), and NLR > 5.8 (41.38%, 72%, 5.66%, 96.80%, 70.81%). Conclusions The use of combined screening methods can classify pregnant women with high-risk COVID-19 before definitively diagnosed with RT-PCR. This practice will help to reduce RT-PCR need in a limited resources country.

scholarly journals Alternative or Complementary Role of Serological Rapid Antibody Test in the Management of Possible COVID-19 Cases

2020 ◽  
Author(s):  
Fatma Yildirim ◽  
Pinar Yildiz Gulhan ◽  
Ozlem Ercen Diken ◽  
Aylin Capraz ◽  
Meltem Simsek ◽  
...  
Keyword(s):  
Antibody Test ◽  
Test Method ◽  
Nasopharyngeal Swab ◽  
Common Symptom ◽  
Rt Pcr ◽  
Igg Antibody ◽  
Serological Tests ◽  
Female Ratio ◽  
The Mean ◽  
Rapid Antibody

Background: Although the gold diagnostic method for COVID-19 is accepted as the detection of viral particles by reverse transcription polymerase chain reaction (RT-PCR), serology testing for SARS-CoV-2 is at increased demand. A primary aim for utilization of serological tests are to better quantify the number of COVID-19 cases including those RT-PCR samples were negative but showing clinical and radiological signs of COVID-19. In this study, we aimed to report the features of the patients that were diagnosed and treated as possible COVID-19 cases whose multiple nasopharyngeal swab samples were negative by RT-PCR but serological IgM/IgG antibody against SARS-CoV-2 were detected by rapid antibody test. Method: We retrospectively analyzed eighty suspected COVID-19 cases that have at least two negative consecutive COVID-19 PCR test and were subjected to serological rapid antibody test. Result: The specific antibodies against SARS-CoV-2 were detected as positive in twenty-two patients. The mean age of patient group was 63.2+-13.1 years old with male /female ratio 11/11. Cough was the most common symptom with 90.9%. Most common presenting chest CT findings were bilateral ground glass opacities (77.2%) and alveolar consolidations (50.09%). The mean duration from symptom initiation to hospital admission, to hospitalization, to treatment initiation and to detection of antibody positivity were 8.6 +- 7.2, 11.2 +- 5.4, 7.9 +- 3.2 and 24 +- 17 days, respectively. Conclusion: Our study demonstrated the feasibility of COVID-19 diagnosis based on rapid antibody test in the cases of patients whose RT-PCR samples were negative. We suggest that the detection of antibodies against SARS-CoV-2 with rapid antibody test should be included in the diagnostic algorithm in suspected COVID-19 patients.

scholarly journals Evaluation of Performance of Detection of IgG and IgM Antibody Against Spike Protein of SARS-CoV-2 by a Rapid Kit in a Real-Life Hospital Setting

2021 ◽  
Author(s):  
Monica Irungbam ◽  
Anubhuti Chitkara ◽  
Vijay Kumar Singh ◽  
Subash Chandra Sonkar ◽  
Abhisekh Dubey ◽  
...  
Keyword(s):  
Real Life ◽  
Hospital Setting ◽  
Antibody Test ◽  
Protein S ◽  
Spike Protein ◽  
Diagnostic Efficiency ◽  
Rt Pcr ◽  
Antibody Testing ◽  
Diagnostic Efficacy ◽  
Rapid Antibody

Abstract Background Antibody testing are often used for serosurveillance of COVID-19. ELISA and Chemiluminesence based antibody test are quiet sensitive and specific for such serological testing. Rapid antibody tests are developed and effectively used for this purpose. But their diagnostic efficiency needs to be evaluated. So, the present study was conducted in a dedicated COVID-19 hospital in Delhi, India to evaluate the diagnostic efficacy of a Rapid antibody kit for COVID-19. Material and Method : Sixty COVID-19 confirmed cases by RT-PCR were recruited and categorized as early, intermediate and late cases based on the number of days of their first RT-PCR + ve tests, 20 subjects in each category. Twenty samples from pre-covid era were taken as controls. IgM and IgG antibodies against RBD of spike protein (S) of SARS-CoV2 virus were detected by Rapid antibody test and compared with total antibody against the nucleocapsid (N) antigen of SARS-CoV-2 by Electrochemiluminescence based Immunoassay (ECLIA). Results The detection IgM against Receptor binding domain (RBD) of spike protein by rapid kit was 0-37.5% sensitive and 0-100% specific for diagnosis of SARS-CoV-2 infection. However, efficacy of detection of IgG by rapid kit was 87–89% sensitive and 75–100% specific when compared with total antibody against N antigen measured by ECLIA based immunoassay. Conclusion It can be concluded that detection of IgM against RBD of S protein by rapid kit is not effective but IgG detection can be used as an effective diagnostic tool for SARS-COV-2 infection.

scholarly journals Perbandingan Metode RT-PCR dan Tes Rapid Antibodi untuk Deteksi COVID-19

2020 ◽  
Vol 6 (Khusus) ◽  
pp. 47
Author(s):  
Anita Suswanti Agustina ◽  
Rizana Fajrunni'mah
Keyword(s):  
Rapid Test ◽  
Antibody Test ◽  
Rt Pcr ◽  
Literature Study ◽  
Antibody Testing ◽  
Advantages And Disadvantages ◽  
Pcr Method ◽  
Risk Of Exposure ◽  
Antibody Tests ◽  
Rapid Antibody

COVID-19 is caused by SARS-CoV-2, which can spread rapidly from human to human. There are several laboratory tests to detect COVID-19, including the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method and a rapid test antibody test to detect antibody reactions to SARS-CoV-2. Both methods have advantages and disadvantages of each, while the literature study comparing the two methods is currently limited. The type of research used is library research. Research materials have been collected from various journals, books, and guidelines, in line with the research topic, to obtain 24 library sources. The results of the literature study indicate that the target genes that can be used to detect COVID-19 RT-PCR methods include the N, E, RdRp, and ORF1a/b genes. The sensitivity of rapid antibody tests is known to range from 68−89%, while the specificity of rapid antibody tests ranges from 91−100%. RT-PCR has the advantage of being able to detect low-concentration antigens, but RT-PCR has weaknesses such as requiring expensive equipment and inspection fees, specially trained laboratory personnel, long working time, and high risk of exposure. Rapid antibody testing has advantages, including ease of sampling, lower testing costs, reduced risk of exposure to officers, does not require special equipment and space, but has the potential for cross-reactivity with other coronaviruses. Both the RT-PCR method and the rapid antibody test have their advantages and disadvantages, but rapid antibody testing with RT-PCR can improve the diagnosis of COVID-19. The results of this literature study are expected to be continued as a basis for further research on RT-PCR examination and antibody rapid test for COVID-19 detection in Indonesia, accompanied by information on onset time and time-testing with a large sample of research.

scholarly journals Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection

2020 ◽  
Vol 9 (1) ◽  
pp. 78-86
Author(s):  
Ömer ACER ◽  
Osman ÖZÜDOĞRU
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Antibody Test ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Igg Antibody ◽  
Positive Ratio ◽  
Severe Group ◽  
Polymerase Chain ◽  
Pcr Test

In the present study, we compared the real-time reverse-transcription polymerase chain reaction (RT-PCR) and total IgM-IgG antibody tests for diagnosis SARS-CoV-2 patients. SARS-CoV-2 patients were divided into four additional subgroups according to clinical examination, and Computed Tomography (CT) for SARS-CoV-2. Groups were included 60 mild cases, 111 moderate cases, 53 severe cases, and 105 normal cases. In a mild group, 52.5% of 60 cases were found to be male, 45.9% female, and the average age was found as 38.4 ±2.011. The positive ratio was found as 80.3% in the RT-PCR test, while 39.3% in total IgM/IgG. In a moderate group, 49.1% of 111 cases were found to be male, 50% female, and the average age was 45.05 ±1.519. The positive ratio was found as 85.7% in the RT-PCR test while 54.5% in total IgM/IgG. In a severe group, 53.7%of 53 cases were found to be male, 44.4% female, and the average age was 55.5±2.122. The positive ratio was found as 75.9% both in RT-PCR and total IgM/IgG tests. In a normal group with no involvement according to Computed Tomography (CT), 49.1%of 105 cases were found to be male, 50% female, and the average age was found as 34.8±1.391. The positive ratio was found as 95.3% in the RT-PCR test while 5.7% in total IgM/IgG. Chronic diseases were detected more in severe cases, suggesting that persons who have chronic diseases or decreased immunity, such as diabetes mellitus, cardiovascular diseases, hypertension, and lung disease, are at a higher risk for developing severe COVID-19 if they are infected with SARS-CoV-2. According to our results, we can suggest a higher detection sensibility in RT-PCR than in total IgM/IgG antibody test for mild, moderate and normal group, while the detection sensibility of IgM/IgG antibody increases in a severe group with bilateral diffuse involvement according to CT.

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