Establishment of a long‐term hypertrophic scar model by injection of anhydrous alcohol: A rabbit model

2021 ◽  
Author(s):  
Wenxuan Zu ◽  
Banghong Jiang ◽  
Hongwei Liu
Keyword(s):  
Hypertrophic Scar ◽  
Rabbit Model ◽  
Anhydrous Alcohol

Effect of Artesunate Combined With Fractional CO 2 Laser on the Hypertrophic Scar in a Rabbit Model

2021 ◽  
Author(s):  
Jinxia Zhang ◽  
Zhikuan Xia ◽  
Shuanglin Zhou ◽  
Wanting Luo ◽  
Zhuoying Peng ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Rabbit Model

Long-term total intraperitoneal nutrition in a rabbit model

1986 ◽  
Vol 21 (3) ◽  
pp. 267-270 ◽  
Author(s):  
Marshall M. Stone ◽  
Sean J. Mulvihill ◽  
Klaus J. Lewin ◽  
Eric W. Fonkalsrud
Keyword(s):  
Rabbit Model

scholarly journals High-Dose Daptomycin Is Effective as an Antibiotic Lock Therapy in a Rabbit Model of Staphylococcus epidermidis Catheter-Related Infection

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Jana Basas ◽  
Marta Palau ◽  
Carlos Ratia ◽  
José L. del Pozo ◽  
María Teresa Martín-Gómez ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Rabbit Model ◽  
Bloodstream Infections ◽  
High Dose ◽  
Coagulase Negative Staphylococci ◽  
Poor Patient ◽  
Lock Therapy ◽  
Antibiotic Lock ◽  
High Treatment

ABSTRACT Long-term catheter-related bloodstream infections (CRBSIs) involving coagulase-negative staphylococci are associated with poor patient outcomes, increased hospitalization, and high treatment costs. The use of vancomycin lock therapy has been an important step forward in treatment of these biofilms, although failures occur in 20% of patients. In this study, we report that a high dose of daptomycin lock therapy may offer a therapeutic advantage for these CRBSIs in just 24 h of treatment.

Long-Term Histologic Studies of the Baerveldt Implant in a Rabbit Model

1996 ◽  
Vol 5 (5) ◽  
pp. 334???339 ◽  
Author(s):  
Mary Ann Lloyd ◽  
George Baerveldt ◽  
Quang H. Nguyen ◽  
Don S. Minckler
Keyword(s):  
Rabbit Model ◽  
Baerveldt Implant

Orchestrated biomechanical, structural, and biochemical stimuli for engineering anisotropic meniscus

2019 ◽  
Vol 11 (487) ◽  
pp. eaao0750 ◽  
Author(s):  
Zheng-Zheng Zhang ◽  
You-Rong Chen ◽  
Shao-Jie Wang ◽  
Feng Zhao ◽  
Xiao-Gang Wang ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Proper Function ◽  
Type I ◽  
Specific Expression ◽  
Tissue Organization ◽  
Meniscus Tissue ◽  
Biomimetic Scaffold ◽  
And Function

Reconstruction of the anisotropic structure and proper function of the knee meniscus remains an important challenge to overcome, because the complexity of the zonal tissue organization in the meniscus has important roles in load bearing and shock absorption. Current tissue engineering solutions for meniscus reconstruction have failed to achieve and maintain the proper function in vivo because they have generated homogeneous tissues, leading to long-term joint degeneration. To address this challenge, we applied biomechanical and biochemical stimuli to mesenchymal stem cells seeded into a biomimetic scaffold to induce spatial regulation of fibrochondrocyte differentiation, resulting in physiological anisotropy in the engineered meniscus. Using a customized dynamic tension-compression loading system in conjunction with two growth factors, we induced zonal, layer-specific expression of type I and type II collagens with similar structure and function to those present in the native meniscus tissue. Engineered meniscus demonstrated long-term chondroprotection of the knee joint in a rabbit model. This study simultaneously applied biomechanical, biochemical, and structural cues to achieve anisotropic reconstruction of the meniscus, demonstrating the utility of anisotropic engineered meniscus for long-term knee chondroprotection in vivo.

scholarly journals Long‐term prevention of capsular opacification after lens‐refilling surgery in a rabbit model

2019 ◽  
Vol 97 (6) ◽  
Author(s):  
Theo G. van Kooten ◽  
Steven A. Koopmans ◽  
Thom Terwee ◽  
Sönke Langner ◽  
Oliver Stachs ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Lens Refilling

Long-term neurological effects of neonatal caffeine treatment in a rabbit model of preterm birth

2019 ◽  
Vol 87 (6) ◽  
pp. 1011-1018
Author(s):  
Lennart Van der Veeken ◽  
Susanne Grönlund ◽  
Erik Gerdtsson ◽  
Bo Holmqvist ◽  
Jan Deprest ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Rabbit Model ◽  
Caffeine Treatment ◽  
Neurological Effects

The Plasticity of Myeloma Plasma Cells as Expressed by Dedifferentiation into an Immature, Resilient, Apoptosis-Resistant Phenotype.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 633-633 ◽  
Author(s):  
Shmuel Yaccoby ◽  
Kenichiro Yata ◽  
Yun Ge ◽  
Bart Barlogie ◽  
Guido Tricot ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rabbit Model ◽  
Common Mechanism ◽  
Proliferative Potential ◽  
Apoptotic Cells ◽  
Apoptosis Resistance ◽  
Hematopoietic Stem ◽  
Induced Apoptosis

Abstract Progression of myeloma (MM) is considered to be a multistage and dynamic process of cell differentiation, survival, proliferation and dissemination. We have previously demonstrated the proliferative potential of purified CD45lowCD38high mature MM cells in SCID-hu mice (Yaccoby & Epstein, Blood, 1999), the ability of CD138-selected MM cells to produce myeloma in our novel SCID-rabbit model (Yata et al., ASH, 2003), and the interdependence of MM bone disease and tumor growth whereby MM cells induce osteoclast activity and are dependent on osteoclasts in vivo (Yaccoby et al., BJH, 2002) and ex vivo (Yaccoby et al., Cancer Res., 2004). The aim of this study was to determine the osteoclast-induced phenotypic changes associated with survival of MM cells in long term co-culture. CD138-selected (>95% purity) MM cells from 16 patients were co-cultured with human osteoclasts for up to 20 weeks. The pre-cultured baseline cells were typically CD45low/inermediateCD38high, CD19−CD34−. At the end of long term co-culture (>6 weeks) MM cells had BrdU labeling index (LI) of 2.5±2.0 and their viability was 97%±1%. The phenotype of co-cultured MM cells consistently shifted to a less mature phenotype, with CD45 expression increasing from CD45low to CD45intermmediata/high and reduced expression of CD38 from CD38high to subpopulations with CD38intermediate levels, as determined by flow cytometry and confirmed by qRT-PCR. Further flow analysis revealed that co cultured MM cells also expressed low levels of CD19 and CD34, and identified a small subpopulation of CD138lowCD45high MM cells. Morphologically, the co-cultured MM cells uniformly gained plasmablastic characteristics when compared to pre-cultured cells. Previous reports suggested that IL-6 was important for maintaining subpopulation of CD45-expressing MM cells. However, blocking IL-6 activity in co-cultures with anti-IL6 and anti-IL6R neutralizing antibodies (5 μg/ml, each) did not affect the immature phenotype of MM cells. Intriguingly, long term co-culture of normal CD34+ hematopoietic stem cells (HSCs) with osteoclasts results in loss of CD34 expression, suggesting a common mechanism for osteoclast-induced MM PC and HSC plasticity. To investigate if the observed phonotypic changes are associated with apoptosis resistance, we determined the effects of 3 days exposure to the pro-apoptotic agent dexamethasone (DEX, 10−7 M) on MM cells cultured alone or in co-cultures (n=5), at initiation (baseline) and after 6 weeks of co-cultures. The percent apoptotic cells was determined by trypan blue exclusion and annexin V flow cytometry. When baseline MM cells were cultured alone, DEX significantly increased the percent of apoptotic cells over that spontaneous rate (p<0.01). In contrast, when MM cells recovered from co-cultures after 6 weeks they survived and were resistant to DEX-induced apoptosis (16%±11% and 24%±21% apoptotic cells in the absence and presence of DEX, respectively). As reported, osteoclasts supported survival of MM cells at baseline and after 6 weeks of co-culture (p<0.01), and protected MM PCs from DEX-induced apoptosis. Our data demonstrate the phenotypic plasticity of primary myeloma cells, whereby mature MM cells are reprogrammed and acquire autonomous survival properties after co-culture with osteoclasts. We hypothesize that in vivo these cells are dormant, resistant to spontaneous and drug-induced apoptosis, and could be responsible for relapse.

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