scholarly journals Specific knock-down of tissue non-specific alkaline phosphatase mRNA levels inhibits intracellular lipid accumulation in 3T3-L1 and HepG2 cells

2017 ◽  
Vol 98 (5) ◽  
pp. 260-268 ◽  
Author(s):  
George Chirambo ◽  
Chantal van Niekerk ◽  
Nigel J. Crowther
Keyword(s):  
Alkaline Phosphatase ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Mrna Levels ◽  
Intracellular Lipid ◽  
Specific Alkaline Phosphatase ◽  
Knock Down ◽  
Intracellular Lipid Accumulation

scholarly journals Zanthoxylum ailanthoides Suppresses Oleic Acid-Induced Lipid Accumulation through an Activation of LKB1/AMPK Pathway in HepG2 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Eun-Bin Kwon ◽  
Myung-Ji Kang ◽  
Soo-Yeon Kim ◽  
Yong-Moon Lee ◽  
Mi-Kyeong Lee ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Methanol Extract ◽  
De Novo ◽  
Oxygen Species ◽  
Gene Expressions ◽  
Intracellular Lipid ◽  
Ampk Signaling ◽  
Intracellular Lipid Accumulation

Zanthoxylum ailanthoides (ZA) has been used as folk medicines in East Asian and recently reported to have several bioactivity; however, the studies of ZA on the regulation of triacylglycerol (TG) biosynthesis have not been elucidated yet. In this study, we examined whether the methanol extract of ZA (ZA-M) could reduce oleic acid- (OA-) induced intracellular lipid accumulation and confirmed its mode of action in HepG2 cells. ZA-M was shown to promote the phosphorylation of AMPK and its upstream LKB1, followed by reduction of lipogenic gene expressions. As a result, treatment of ZA-M blocked de novo TG biosynthesis and subsequently mitigated intracellular neutral lipid accumulation in HepG2 cells. ZA-M also inhibited OA-induced production of reactive oxygen species (ROS) and TNF-α, suggesting that ZA-M possess the anti-inflammatory feature in fatty acid over accumulated condition. Taken together, these results suggest that ZA-M attenuates OA-induced lipid accumulation and inflammation through the activation of LKB1/AMPK signaling pathway in HepG2 cells.

Ethnic differences in pre-adipocyte intracellular lipid accumulation and alkaline phosphatase activity

2015 ◽  
Vol 438 ◽  
pp. 382-387 ◽  
Author(s):  
Aus T. Ali ◽  
George Chirambo ◽  
Clement Penny ◽  
Janice E. Paiker ◽  
Faisel Ikram ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Ethnic Differences ◽  
Alkaline Phosphatase Activity ◽  
Lipid Accumulation ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

scholarly journals Inhibitory Effects of Ethyl Acetate-Soluble Fraction from Morus alba on Lipid Accumulation in 3T3-L1 Cells

2013 ◽  
Vol 8 (11) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Hee-Sook Park ◽  
Soon-Mi Shim ◽  
Gun-Hee Kim
Keyword(s):  
Ethyl Acetate ◽  
Lipid Accumulation ◽  
Soluble Fraction ◽  
Morus Alba ◽  
Response To Treatment ◽  
Mrna Levels ◽  
Asian Countries ◽  
Inhibitory Effects ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

Fruits of mulberry ( Morus alba) have been widely used for therapeutic purposes in Asian countries for centuries. Treatment of 3T3-L1 cells with ethanolic extracts of M. alba decreased adipocyte differentiation at 100 μg/mL by 18.6%. Treatment suppressed mRNA levels of PPARγ and C/EBPα expression in 3T3-L1 cells. However, the extract did not change free glycerol release from mature adipocytes. Thus, M. alba inhibited lipid accumulation by regulating transcription factors in 3T3-L1 adipocytes without a lipolytic effect. Among the soluble- fractions, the ethyl acetate-soluble fraction had the highest anti-adipogenic effects on 3T3-L1 cells. This fraction decreasing intracellular lipid accumulation by 38.5% in response to treatment with 100 μg/mL. In addition, HPLC analysis of the ethyl acetate-soluble fraction of M. alba contained 167.7 μM of protocatechulic acid in 1 mg/mL of fraction, which inhibited lipid accumulation by 44.8% in response to treatment with 100 μM. From these results, M. alba is a possible candidate for regulating lipid accumulation in obesity.

scholarly journals Inhibitory Activity of Intracellular Lipid Accumulation by Various Marine Extracts in HepG2 Cells

2012 ◽  
Vol 44 (3) ◽  
pp. 362-366 ◽  
Author(s):  
Byoung-Mok Kim ◽  
Ji-Hee Jung ◽  
Dong-Soo Kim ◽  
Young-Myoung Kim ◽  
In-Hak Jeong
Keyword(s):  
Inhibitory Activity ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

scholarly journals Posidonia oceanica (L.) Delile Extract Reduces Lipid Accumulation through Autophagy Activation in HepG2 Cells

2021 ◽  
Vol 14 (10) ◽  
pp. 969
Author(s):  
Marzia Vasarri ◽  
Emanuela Barletta ◽  
Donatella Degl’Innocenti
Keyword(s):  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Posidonia Oceanica ◽  
Intracellular Lipid ◽  
Alcoholic Fatty Liver ◽  
Ribosomal Protein S6 ◽  
Intracellular Lipid Accumulation ◽  
Human Hepatoma Hepg2 Cells ◽  
Lipid Reduction ◽  
The Relationship

Posidonia oceanica (L.) Delile is a marine plant traditionally used as an herbal medicine for various health disorders. P. oceanica leaf extract (POE) has been shown to be a phytocomplex with cell-safe bioactivities, including the ability to trigger autophagy. Autophagy is a key pathway to counteract non-alcoholic fatty liver disease (NAFLD) by controlling the breakdown of lipid droplets in the liver. The aim of this study was to explore the ability of POE to trigger autophagy and reduce lipid accumulation in human hepatoma (HepG2) cells and then verify the possible link between the effect of POE on lipid reduction and autophagy activation. Expression levels of autophagy markers were monitored by the Western blot technique in POE-treated HepG2 cells, whereas the extent of lipid accumulation in HepG2 cells was assessed by Oil red O staining. Chloroquine (CQ), an autophagy inhibitor, was used to study the relationship between POE-induced autophagy and intracellular lipid accumulation. POE was found to stimulate an autophagy flux over time in HepG2 cells by lowering the phosphorylation state of ribosomal protein S6, increasing Beclin-1 and LC3-II levels, and decreasing p62 levels. By blocking autophagy with CQ, the effect of POE on intracellular lipid accumulation was clearly reversed, suggesting that the POE phytocomplex may reduce lipid accumulation in HepG2 cells by activating the autophagic process. This work indicates that P. oceanica may be considered as a promising molecule supplier to discover new natural approaches for the management of NAFLD.

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