The relationship between alkaline phosphatase activity and intracellular lipid accumulation in murine 3T3-L1 cells and human preadipocytes

2006 ◽  
Vol 354 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Aus T. Ali ◽  
Clem B. Penny ◽  
Janice E. Paiker ◽  
George Psaras ◽  
Faisel Ikram ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lipid Accumulation ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation ◽  
Human Preadipocytes ◽  
The Relationship

Ethnic differences in pre-adipocyte intracellular lipid accumulation and alkaline phosphatase activity

2015 ◽  
Vol 438 ◽  
pp. 382-387 ◽  
Author(s):  
Aus T. Ali ◽  
George Chirambo ◽  
Clement Penny ◽  
Janice E. Paiker ◽  
Faisel Ikram ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Ethnic Differences ◽  
Alkaline Phosphatase Activity ◽  
Lipid Accumulation ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

scholarly journals VP19.11: Evaluation of the relationship between salivary alkaline phosphatase activity and stress in pregnant women

2021 ◽  
Vol 58 (S1) ◽  
pp. 178-178
Author(s):  
M. Barati ◽  
S. Aberoumand ◽  
M. Aberoumand ◽  
M. Norbehahani ◽  
F. Baesi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
The Relationship

scholarly journals Posidonia oceanica (L.) Delile Extract Reduces Lipid Accumulation through Autophagy Activation in HepG2 Cells

2021 ◽  
Vol 14 (10) ◽  
pp. 969
Author(s):  
Marzia Vasarri ◽  
Emanuela Barletta ◽  
Donatella Degl’Innocenti
Keyword(s):  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Posidonia Oceanica ◽  
Intracellular Lipid ◽  
Alcoholic Fatty Liver ◽  
Ribosomal Protein S6 ◽  
Intracellular Lipid Accumulation ◽  
Human Hepatoma Hepg2 Cells ◽  
Lipid Reduction ◽  
The Relationship

Posidonia oceanica (L.) Delile is a marine plant traditionally used as an herbal medicine for various health disorders. P. oceanica leaf extract (POE) has been shown to be a phytocomplex with cell-safe bioactivities, including the ability to trigger autophagy. Autophagy is a key pathway to counteract non-alcoholic fatty liver disease (NAFLD) by controlling the breakdown of lipid droplets in the liver. The aim of this study was to explore the ability of POE to trigger autophagy and reduce lipid accumulation in human hepatoma (HepG2) cells and then verify the possible link between the effect of POE on lipid reduction and autophagy activation. Expression levels of autophagy markers were monitored by the Western blot technique in POE-treated HepG2 cells, whereas the extent of lipid accumulation in HepG2 cells was assessed by Oil red O staining. Chloroquine (CQ), an autophagy inhibitor, was used to study the relationship between POE-induced autophagy and intracellular lipid accumulation. POE was found to stimulate an autophagy flux over time in HepG2 cells by lowering the phosphorylation state of ribosomal protein S6, increasing Beclin-1 and LC3-II levels, and decreasing p62 levels. By blocking autophagy with CQ, the effect of POE on intracellular lipid accumulation was clearly reversed, suggesting that the POE phytocomplex may reduce lipid accumulation in HepG2 cells by activating the autophagic process. This work indicates that P. oceanica may be considered as a promising molecule supplier to discover new natural approaches for the management of NAFLD.

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