Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

Helicobacter ◽  
2020 ◽  
Vol 25 (5) ◽  
Author(s):  
Eun Jeong Gong ◽  
Ji Yong Ahn ◽  
Da Kyung Jung ◽  
Sun Mi Lee ◽  
Gyu Young Pih ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Rapid Urease Test ◽  
Test Kit ◽  
Urease Test

scholarly journals Prevalance and association of Helicobater Pylori with gastro-duodenal mucosal lesions in patients with dyspepsia

2020 ◽  
Vol 28 (1) ◽  
pp. 100-104
Author(s):  
Abu Raihan Siddique ◽  
Liakat Hossain ◽  
Mushtaque Ahmad Rana ◽  
Probir Kumar Banerjee ◽  
Anisur Rahman ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Wide Spectrum ◽  
Statistical Significance ◽  
P Value ◽  
Rapid Urease Test ◽  
Medical College ◽  
Test Kit ◽  
Urease Test ◽  
Dyspeptic Symptoms ◽  
Mucosal Lesions

Helicobacter Pylori a very common worldwide infection is responsible for number of upper GIT lesions like gastritis, peptic ulcer disease, gastric carcinoma and mucosa associated lymphoid tissue lymphoma. On the other hand, dyspepsia is a wide spectrum of nonspecific upper GIT symptoms for which patients very frequently visit gastroenterology clinics. The objective of the study was to find prevalence of Helicobacter Pylori among the patients with dyspeptic symptoms who had gastro-duodenal mucosal lesions on upper GIT endoscopy and also if there is any association between these two variables. 164 cases of dyspepsia were done index upper GIT endoscopy as per inclusion and exclusion criteria from April 2018 to December 2018 in Seikh Sayera Khatun Medical College Hospital, Gopalganj. Two gastric biopsies (from antrum and body) were taken and immediately placed in a commercial RUT (rapid urease test) kit. Positive test (rapid urease test) was indicated by change in the color of the kit from yellow to pink or red within 24 hours. Out of 164 patients, 62 (37.8%) patients had gastro-duodenal mucosal lesions and rest(62.2%) showed normal upper GIT at endoscopy. Among the 62 patients highest (38) number of the patients endoscopy showed gastritis and least (3) showed gastric ulcer. Among 62 patients with endoscopic gastro-duodenal mucosal lesions, 36 patients were infected with Helicobacter Pylori. On the contrary 27 patients out of 102 subjects who had normal upper GIT at endoscopy recorded infected with Helicobacter Pylori. Statistically analysis was done using chi-square test with the help of SPSS software windows version 25. The level of statistical significance was set at 0.05. In our study it was observed that statistically there was significant relation of Helicobacter Pylori with gastro-duodenal mucosal lesions as P value was less than 0.05. In this study, we found that Helicobacter Pylori was significantly associated with gastro-duodenal mucosal lesions among the patients with dyspeptic symptoms. So, our suggestion will be to confirm H. pylori infection if anybody has gastro-duodenal mucosal lesions if a subject does upper GIT endoscopy for dyspeptic symptoms. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 100-104

scholarly journals Evaluation of a Novel Stool Antigen Rapid Test Kit for Detection of Helicobacter pylori Infection

2018 ◽  
Vol 57 (3) ◽  
Author(s):  
Toshihiko Kakiuchi ◽  
Masumi Okuda ◽  
Kazutoshi Hashiguchi ◽  
Ichiro Imamura ◽  
Aiko Nakayama ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Isolate ◽  
Rapid Test ◽  
Intestinal Bacteria ◽  
Cross Reactivity ◽  
Rapid Urease Test ◽  
Content Type ◽  
Test Kit ◽  
Urease Test ◽  
H Pylori

ABSTRACT The Quick Chaser H. pylori (QCP), which is a novel antigen detection kit for Helicobacter pylori, was developed recently. The previous examination kits targeted the catalase of H. pylori; however, this new test kit, to the best of our knowledge, is the first kit to target the flagellar protein of H. pylori. The present study aimed to evaluate the efficacy of QCP compared with that of the already commercially available rapid test kit Testmate Rapid Pylori Antigen (TRP). TRP and QCP were utilized in 71 participants. The positive and negative concordance ratios of QCP to TRP were 100% (57/57) and 92.9% (13/14), respectively. Sensitivity based on the rapid urease test and culture test was 92.3%. Results of the dilution sensitivity test showed that QCP was eight times more sensitive to an H. pylori standard strain and the clarithromycin (CAM)-resistant clinical isolate and four times more sensitive to a CAM-susceptible clinical isolate than TRP. For the cross-reactivity test, 27 strains that exist in human feces, such as the Helicobacter genus, Bifidobacterium genus, Lactobacillus genus, and other resident bacteria, were selected. The results obtained using QCP were all negative, and no cross-reaction was observed. In conclusion, compared with TRP, QCP can detect H. pylori using clinical specimens with high sensitivity, regardless of CAM susceptibility and tolerance. No cross-reactivity was observed with other intestinal bacteria in humans. The kit is considered extremely useful in clinical settings.

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

scholarly journals Incidence of Gastric Metaplasia and Helicobacter pylori Infection in Duodenal Bulb – With Specific Reference to Patients With Duodenal Ulcers

1999 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Minoru Kawaguchi ◽  
Toshihiko Saito
Keyword(s):  
Helicobacter Pylori ◽  
Duodenal Bulb ◽  
Mucosal Injury ◽  
Transitional Zone ◽  
Specific Reference ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Gastric Metaplasia ◽  
Urease Test ◽  
H Pylori

We determined the incidence of gastric metaplasia in the duodenal bulb of duodenal ulcer patients and the Helicobacter pylori (H. pylori) infection rate at sites with gastric metaplasia. Biopsy of the duodenal bulb showed the presence of gastric metaplasia in 61 of 86 patients (71%) overall and in 18 of 47 patients (38.3%) who had gastrectomy at an early gastric cancer. The histological diagnosis of H. pylori infection showed good agreement (83.3%) with the result of the rapid urease test, indicating that H. pylori occurs in regions with gastric metaplasia. This finding suggests that H. pylori infects gastric metaplasia in the duodenal bulb, causing mucosal injury, which is then transformed into duodenal ulcers. The exact mechanism by which gastric metaplasia is caused is unknown, but it is believed to occur in the transitional zone in the duodenal mucosa.

scholarly journals Helicobacter pylori as a risk factor for gastric cancer: the evidence and primary prevention strategy

2019 ◽  
Vol 47 (6) ◽  
pp. 535-547 ◽  
Author(s):  
I. N. Voynovan ◽  
Yu. V. Embutnieks ◽  
D. V. Mareeva ◽  
S. V. Kolbasnikov ◽  
D. S. Bordin
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Risk Factor ◽  
Primary Prevention ◽  
Russian Federation ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
First Line ◽  
Urease Test ◽  
The Russian Federation

Russia is a country with a high prevalence of Helicobacter pylori (HP) infection, a high incidence of gastric cancer, and its late diagnosis. HР infection has been recognized as the leading manageable risk factor for gastric cancer. Accurate diagnostic tests must be used to identify and control the effectiveness of HP eradication, and effective schemes must be implemented for HP eradication. The aim of this article was to analyze the latest consensus documents, systematic reviews and meta-analyzes that reflected the role of HP as a risk factor for the development of gastric cancer, as well as measures for the risk reduction. We describe in detail the diagnostic methods for HP infection, provide data on their use in the Russian Federation, and analyze the efficacy of eradication regimens. In all HPinfected individuals, HP leads to chronic inflammation in the gastric mucosa and launches a precancerous cascade (Correa's cascade). The risk of gastric cancer increases with severe atrophy, intestinal metaplasia and dysplasia. Primary prevention of gastric cancer is most effective if the eradication is performed before atrophic gastritis develops. The available consensus documents underline the importance of HP infection identification by accurate diagnostics at this stage of chronic gastritis. In Russia, the primary HP diagnosis is based on histology (37.7%), rapid urease test (29.2%), and serology (29.7%). HP stool antigen test (31.3%), 13C-urea breath test (23.4%) and the histological method (23.3%) are most often used to control eradication. Currently, the first line of eradication therapy is recommended as triple therapy with clarithromycin prescribed for 14 days. It is recommended to use double dose of proton pump inhibitors and bismuth to increase the effectiveness of this scheme. A 14-days triple regimen enhanced by bismuth has been recommended as the first-line therapy in the Russian Federation.

scholarly journals Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi

2021 ◽  
Author(s):  
Nansi López-Valverde ◽  
◽  
Antonio López-Valverde ◽  
Ana Suarez ◽  
Bruno Macedo de Sousa ◽  
...  
Keyword(s):  
Systematic Review ◽  
Helicobacter Pylori ◽  
Clinical Studies ◽  
Periodontal Diseases ◽  
Meta Analysis ◽  
Helicobacter Pylori Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rapid Urease Test ◽  
Common Denominator ◽  
Urease Test

Review question / Objective: Is gastric helicobacter pylori infection related to periodontal diseases? Condition being studied: Therefore, the aim of this systematic review and meta-analysis was to identify and analyze clinical studies to determine the direct correlation between Helicobacter Pylori gastric infection andPeriodontal Disease. Study designs to be included: Clinical studies that provided data on Helicobacter Pylori infection in both the stomach and oral cavity, confirmed by polymerase chain reaction (PCR), rapid urease test (RUT) or enzyme-linked immunosorbent assay (ELISA). Clinical studies that associated PD with Helicobacter Pylori. The diagnosis of PD was confirmed ac-cording to the diagnostic criteria in periodontology.

scholarly journals Can probiotics improve efficiency and safety profile of triple Helicobacter pylori eradication therapy? A prospective randomized study

2016 ◽  
Vol 73 (11) ◽  
pp. 1044-1049 ◽  
Author(s):  
Sasa Grgov ◽  
Tomislav Tasic ◽  
Biljana Radovanovic-Dinic ◽  
Daniela Benedeto-Stojanov
Keyword(s):  
Helicobacter Pylori ◽  
Randomized Study ◽  
Eradication Therapy ◽  
Saccharomyces Boulardii ◽  
Rapid Urease Test ◽  
Group I ◽  
Urease Test ◽  
Probiotic Strains ◽  
The Difference ◽  
H Pylori

Background/Aim. Some studies suggest the benefit of applying different probiotic strains in combination with antibiotics in the eradication of Helicobacter pylori (H. pylori) infection. The aim of this study was to evaluate the effect of co-administration of multiple probiotic strains with triple H. pylori eradication therapy. Methods. This prospective study included 167 patients with dyspeptic symptoms and chronic gastritis who were diagnosed with H. pylori infection and randomized into two groups. The group I of 77 patients underwent triple eradication therapy, for 7 days, with lansoprazole, 2 ? 30 mg half an hour before the meal, amoxicillin 2 ? 1.000 mg per 12 hours and clarithromycin 2 ? 500 mg per 12 hours. After the 7th day of the therapy, lansoprazole continued at a dose of 30 mg for half an hour before breakfast for 4 weeks. The group II of 90 patients received the same treatment as the patients of the group I, with the addition of the probiotic cultures in the form of a capsule comprising Lactobacillus Rosell-52, Lactobacillus Rosell-11, Bifidobacterium Rosell-1755 and Saccharomyces boulardii, since the beginning of eradication for 4 weeks. Eradication of H. pylori infection control was performed 8 weeks after the therapy by rapid urease test and histopathologic evaluation of endoscopic biopsies or by stool antigen test for H. pylori. Results. Eradication of H. pylori infection was achieved in 93.3% of the patients who received probiotics with eradication therapy and in 81.8% of patients who were only on eradication therapy without probiotics. The difference in eradication success was statistically significant, (p < 0.05). The incidence of adverse effects of eradication therapy was higher in the group of patients who were not on probiotic (28.6%) than in the group that received probiotic (17.7%), but the difference was not statistically significant. Conclusion. Multiple probiotic strains addition to triple eradication therapy of H. pylori achieves a significantly better eradication success, with fewer side effects of antibiotics.

scholarly journals Antibiotic resistance of Helicobacter pylori in the European part of the Russian Federation: first results

2020 ◽  
Vol 92 (8) ◽  
pp. 24-28
Author(s):  
I. V. Maev ◽  
D. N. Andreev ◽  
V. M. Govorun ◽  
E. N. Ilina ◽  
Yu. A. Kucheryavyy ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Russian Federation ◽  
European Part ◽  
Diffusion Method ◽  
Rapid Urease Test ◽  
Urease Test ◽  
First Results ◽  
The Russian Federation ◽  
H Pylori

Aim. Determine the primary antibiotic resistance of Helicobacter pylori (H. pylori) strains isolated from patients living in the European part of the Russian Federation. Materials and methods. As part of a clinical laboratory study, from 2015 to 2018, 27 gastrobiopsy samples obtained from H. pylori-infected patients were analyzed. H. pylori infection was verified using a rapid urease test or a 13C-urea breath test. The values of the minimum inhibitory concentration (MIC) of antibiotics were determined by the diffusion method using E-test strips (BioMerieux, France) according to the recommendations of the manufacturer. The sensitivity of the isolates was determined for 6 antibacterial drugs (amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline, rifampicin). Results. According to the data obtained, resistance to amoxicillin was 0%, clarithromycin 11.1%, metronidazole 59.3%, levofloxacin 3.7%, tetracycline 0%, and rifampicin 14.8%. Dual resistance to clarithromycin and metronidazole was recorded in two isolates (7.4%). Conclusion. Thus, the first results of the evaluation of H. pylori antibiotic resistance in the European part of the Russian Federation indicate a low resistance of the microorganism to clarithromycin and quite high to metronidazole.

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