Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleeding peptic ulcers

2016 ◽  
Vol 48 (8) ◽  
pp. 899-903 ◽  
Author(s):  
Woo Chul Chung ◽  
Eun Jung Jeon ◽  
Jung Hwan Oh ◽  
Jae Myung Park ◽  
Tae Ho Kim ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Multiplex Pcr ◽  
Rapid Urease Test ◽  
Peptic Ulcers ◽  
Tissue Samples ◽  
Dual Priming Oligonucleotide ◽  
Test Kit ◽  
Urease Test ◽  
Bleeding Peptic Ulcers

Endoscopic Diagnosis of Helicobacter pylori Infection by Rapid Urease Test in Bleeding Peptic Ulcers

2009 ◽  
Vol 43 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Jui-Hsiang Tang ◽  
Nai-Jen Liu ◽  
Hao-Tsai Cheng ◽  
Ching-Song Lee ◽  
Yin-Yi Chu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Endoscopic Diagnosis ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Peptic Ulcers ◽  
Urease Test ◽  
Bleeding Peptic Ulcers

Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

Helicobacter ◽  
2020 ◽  
Vol 25 (5) ◽  
Author(s):  
Eun Jeong Gong ◽  
Ji Yong Ahn ◽  
Da Kyung Jung ◽  
Sun Mi Lee ◽  
Gyu Young Pih ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Rapid Urease Test ◽  
Test Kit ◽  
Urease Test

scholarly journals Diagnosis, Treatment, and Outcome in Patients with Bleeding Peptic Ulcers andHelicobacter pyloriInfections

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Ting-Chun Huang ◽  
Chia-Long Lee
Keyword(s):  
Urea Breath Test ◽  
Sufficient Evidence ◽  
Rapid Urease Test ◽  
Peptic Ulcers ◽  
Ulcer Disease ◽  
Urease Test ◽  
Bleeding Peptic Ulcers ◽  
Independent Risk Factors ◽  
Infection Eradication ◽  
Upper Gastrointestinal

Upper gastrointestinal (UGI) bleeding is the most frequently encountered complication of peptic ulcer disease.Helicobacter pylori(Hp) infection and nonsteroidal anti-inflammatory drug (NSAID) administration are two independent risk factors for UGI bleeding. Therefore, testing for and diagnosingHpinfection are essential for every patient with UGI hemorrhage. The presence of the infection is usually underestimated in cases of bleeding peptic ulcers. A rapid urease test (RUT), with or without histology, is usually the first test performed during endoscopy. If the initial diagnostic test is negative, a delayed13C-urea breath test (UBT) or serology should be performed. Once an infection is diagnosed, antibiotic treatment is advocated. Sufficient evidence supports the concept thatHpinfection eradication can heal the ulcer and reduce the likelihood of rebleeding. With increased awareness of the effects ofHpinfection, the etiologies of bleeding peptic ulcers have shifted to NSAID use, old age, and disease comorbidity.

DETECTING POINT MUTATIONS A2142G AND A2143G IN THE 23S RRNA GENE CAUSING HELICOBACTER PYLORI RESISTANCE TO CLARITHROMYCIN BY PCR RFLP

2013 ◽  
pp. 56-63
Author(s):  
Thi Minh Thi Ha ◽  
Van Huy Tran
Keyword(s):  
Helicobacter Pylori ◽  
Point Mutations ◽  
23S Rrna ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
Peptic Ulcers ◽  
Urease Test ◽  
Pcr Rflp ◽  
Pcr Products ◽  
23S Rrna Gene

Background: Clarithromycine-resistance of H.pylori is one important cause of decreasing eradication rate of H.pylori. This study is aimed at: (1): evaluating the application of PCR RFLP in detecting point mutations A2142G and A 2143G in the 23SrRNA gene resulting Clarithromycine-resistant H.pylori. (2) determining the rate of these point mutations by PCR RFLP in patients with gastroduodenitis or peptic ulcers. Patients and methods: 38 patients with gastroduodenitis or peptic ulcer were enrolled. H.pylori infection was confirmed by rapid Urease test and PCR. PCR was performed in two phases: amplification of gene 23S rRNA containing A2143 and A2142, followed by digestion of PCR products by enzymes BbsI and BsaI in order to detecting point mutations A2143G and A2142G. Different quantities of enzyme of digestion were evaluated (5U; 7,5U; 10U; 15U and 20U) in order to determine an optimal quantity. Results: The components of digesting reaction were optimized as followings: performed in a solution volume of 20 µl, including 5 µl PCR products (to amplify gene 23S rRNA containing 2142 and 2143), 10 U enzyme (BsaI to detect A2143G, BbsI to detect A2142G), 2 µl buffer G 2X, and water for a sufficient volume. 17 point mutations A2143G were found (44.7%). Conclusion: PCR RFLP could be routinely applied to detect point mutations A2143G and A2142G in the 23S rRNA gene causing clarithromycine-resistant H.pylori. The rate of these point mutations in this group of patients was relatively high. Key words: Clarithromycin resistance, gene 23S rRNA, Helicobacter pylori

scholarly journals Prevalance and association of Helicobater Pylori with gastro-duodenal mucosal lesions in patients with dyspepsia

2020 ◽  
Vol 28 (1) ◽  
pp. 100-104
Author(s):  
Abu Raihan Siddique ◽  
Liakat Hossain ◽  
Mushtaque Ahmad Rana ◽  
Probir Kumar Banerjee ◽  
Anisur Rahman ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Wide Spectrum ◽  
Statistical Significance ◽  
P Value ◽  
Rapid Urease Test ◽  
Medical College ◽  
Test Kit ◽  
Urease Test ◽  
Dyspeptic Symptoms ◽  
Mucosal Lesions

Helicobacter Pylori a very common worldwide infection is responsible for number of upper GIT lesions like gastritis, peptic ulcer disease, gastric carcinoma and mucosa associated lymphoid tissue lymphoma. On the other hand, dyspepsia is a wide spectrum of nonspecific upper GIT symptoms for which patients very frequently visit gastroenterology clinics. The objective of the study was to find prevalence of Helicobacter Pylori among the patients with dyspeptic symptoms who had gastro-duodenal mucosal lesions on upper GIT endoscopy and also if there is any association between these two variables. 164 cases of dyspepsia were done index upper GIT endoscopy as per inclusion and exclusion criteria from April 2018 to December 2018 in Seikh Sayera Khatun Medical College Hospital, Gopalganj. Two gastric biopsies (from antrum and body) were taken and immediately placed in a commercial RUT (rapid urease test) kit. Positive test (rapid urease test) was indicated by change in the color of the kit from yellow to pink or red within 24 hours. Out of 164 patients, 62 (37.8%) patients had gastro-duodenal mucosal lesions and rest(62.2%) showed normal upper GIT at endoscopy. Among the 62 patients highest (38) number of the patients endoscopy showed gastritis and least (3) showed gastric ulcer. Among 62 patients with endoscopic gastro-duodenal mucosal lesions, 36 patients were infected with Helicobacter Pylori. On the contrary 27 patients out of 102 subjects who had normal upper GIT at endoscopy recorded infected with Helicobacter Pylori. Statistically analysis was done using chi-square test with the help of SPSS software windows version 25. The level of statistical significance was set at 0.05. In our study it was observed that statistically there was significant relation of Helicobacter Pylori with gastro-duodenal mucosal lesions as P value was less than 0.05. In this study, we found that Helicobacter Pylori was significantly associated with gastro-duodenal mucosal lesions among the patients with dyspeptic symptoms. So, our suggestion will be to confirm H. pylori infection if anybody has gastro-duodenal mucosal lesions if a subject does upper GIT endoscopy for dyspeptic symptoms. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 100-104

scholarly journals Evaluation of a Novel Stool Antigen Rapid Test Kit for Detection of Helicobacter pylori Infection

2018 ◽  
Vol 57 (3) ◽  
Author(s):  
Toshihiko Kakiuchi ◽  
Masumi Okuda ◽  
Kazutoshi Hashiguchi ◽  
Ichiro Imamura ◽  
Aiko Nakayama ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Isolate ◽  
Rapid Test ◽  
Intestinal Bacteria ◽  
Cross Reactivity ◽  
Rapid Urease Test ◽  
Content Type ◽  
Test Kit ◽  
Urease Test ◽  
H Pylori

ABSTRACT The Quick Chaser H. pylori (QCP), which is a novel antigen detection kit for Helicobacter pylori, was developed recently. The previous examination kits targeted the catalase of H. pylori; however, this new test kit, to the best of our knowledge, is the first kit to target the flagellar protein of H. pylori. The present study aimed to evaluate the efficacy of QCP compared with that of the already commercially available rapid test kit Testmate Rapid Pylori Antigen (TRP). TRP and QCP were utilized in 71 participants. The positive and negative concordance ratios of QCP to TRP were 100% (57/57) and 92.9% (13/14), respectively. Sensitivity based on the rapid urease test and culture test was 92.3%. Results of the dilution sensitivity test showed that QCP was eight times more sensitive to an H. pylori standard strain and the clarithromycin (CAM)-resistant clinical isolate and four times more sensitive to a CAM-susceptible clinical isolate than TRP. For the cross-reactivity test, 27 strains that exist in human feces, such as the Helicobacter genus, Bifidobacterium genus, Lactobacillus genus, and other resident bacteria, were selected. The results obtained using QCP were all negative, and no cross-reaction was observed. In conclusion, compared with TRP, QCP can detect H. pylori using clinical specimens with high sensitivity, regardless of CAM susceptibility and tolerance. No cross-reactivity was observed with other intestinal bacteria in humans. The kit is considered extremely useful in clinical settings.

The impact of the nucleotide-binding oligomerization domain 1 (NOD 1) gene polymorphism on Helicobacter Pylori induced chronic gastritis in hepatic patients.

2021 ◽  
Vol 30 (3) ◽  
pp. 9-19
Author(s):  
Enas M. Ghoneim ◽  
Eman H. Hassan ◽  
Hassan Zaghla ◽  
Doha Taie ◽  
Samah M. Awad
Keyword(s):  
Helicobacter Pylori ◽  
Gene Polymorphism ◽  
Chronic Gastritis ◽  
Nucleotide Binding ◽  
Esophageal Hiatus ◽  
Rapid Urease Test ◽  
Tissue Samples ◽  
Urease Test ◽  
The Impact ◽  
Oligomerization Domain

Background: Chronic infection with Helicobacter pylori (H.pylori ) causes atrophic and even gastric metaplastic changes, and it has a well-known link to peptic ulceration. Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a protein receptor that is presented by the NOD1 gene. It distinguishes H.pylori bacterial molecules and enhances an immune response Objectives: to describe the relation between the NOD1 gene (rs2075820) polymorphism and H.pylori infection in hepatic and non hepatic patients with chronic gastritis, study its impact on the degree of chronic gastritis in H.pylori positive individuals, and to examine the effect of H. pylori on clinical, endoscopic and histopathological findings and child paugh scoring in hepatic patients. Methodology: Gastric tissue samples were taken from selected 200 patients with chronic gastritis, either hepatic or non hepatic. Rapid urease test and pathological findings classified them into H.pylori infected and non infected patients. Genotyping of NOD 1 was studied using polymerase chain reaction /restriction fragment length polymorphism (PCR–RFLP) method. Results: A significant higher frequency of AA genotype, and the A allele of NOD1 gene in H.pylori +ve patients, either hepatic; (58%)-(73%) or non hepatic;(62%)-(78%) as compared to H.pylori –ve patients,(P <0.001). A highly significant relation between NOD1 genotypes and endoscopic findings, where most of H.pylori infected patients with AA genotype had more peptic ulcer, antral erosion, gastric prolapse, esophageal varices and esophageal hiatus hernia compared to patients with GA and GG genotypes, (P<0.001). No significant impact of H.pylori on signs of liver affection and child paugh scoring in hepatic patients. Conclusions: In NOD1 gene polymorphism, AA genotype and A allele have significantly implicated in H.pylori infection susceptibility and progression. While GG genotype and G allele have a protective effect against H.pylori infection.

Bleeding peptic ulcers and presence of Helicobacter pylori by various tests: a case–control study

2002 ◽  
Vol 14 (10) ◽  
pp. 1113-1118 ◽  
Author(s):  
Gonzalo Castillo-Rojas ◽  
M. Arturo Ballesteros ◽  
Sergio Ponce de León ◽  
Rosario Morales-Espinosa ◽  
Alejandro Cravioto ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Case Control Study ◽  
Case Control ◽  
Peptic Ulcers ◽  
Bleeding Peptic Ulcers ◽  
Control Study
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