Gene trapping reveals a new transcriptionally active genome element: The chromosome‐specific clustered trap region

2021 ◽  
Author(s):  
Iyo Takeda ◽  
Masatake Araki ◽  
Kei‐ichiro Ishiguro ◽  
Toshinori Ohga ◽  
Kouki Takada ◽  
...  
Keyword(s):  
Gene Trapping

scholarly journals Proteinuria and Perinatal Lethality in Mice Lacking NEPH1, a Novel Protein with Homology to NEPHRIN

2001 ◽  
Vol 21 (14) ◽  
pp. 4829-4836 ◽  
Author(s):  
Dorit B. Donoviel ◽  
Deon D. Freed ◽  
Hannes Vogel ◽  
David G. Potter ◽  
Edith Hawkins ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transmembrane Protein ◽  
Embryonic Stem ◽  
Congenital Nephrotic Syndrome ◽  
Northern Analysis ◽  
Gene Trapping ◽  
Filtration Barrier ◽  
Perinatal Lethality ◽  
Human Ortholog

ABSTRACT A high-throughput, retrovirus-mediated mutagenesis method based on gene trapping in embryonic stem cells was used to identify a novel mouse gene. The human ortholog encodes a transmembrane protein containing five extracellular immunoglobulin-like domains that is structurally related to human NEPHRIN, a protein associated with congenital nephrotic syndrome. Northern analysis revealed wide expression in humans and mice, with highest expression in kidney. Based on similarity to NEPHRIN and abundant expression in kidney, this protein was designated NEPH1 and embryonic stem cells containing the retroviral insertion in the Neph1 locus were used to generate mutant mice. Analysis of kidney RNA fromNeph1 −/− mice showed that the retroviral insertion disrupted expression of Neph1 transcripts.Neph1 −/− pups were represented at the expected normal Mendelian ratios at 1 to 3 days of age but at only 10% of the expected frequency at 10 to 12 days after birth, suggesting an early postnatal lethality. The Neph1 −/−animals that survived beyond the first week of life were sickly and small but without edema, and all died between 3 and 8 weeks of age. Proteinuria ranging from 300 to 2,000 mg/dl was present in allNeph1 −/− mice. Electron microscopy demonstrated NEPH1 expression in glomerular podocytes and revealed effacement of podocyte foot processes inNeph1 −/− mice. These findings suggest that NEPH1, like NEPHRIN, may play an important role in maintaining the structure of the filtration barrier that prevents proteins from freely entering the glomerular urinary space.

High throughput gene trapping and postinsertional modifications of gene trap alleles

Methods ◽  
2011 ◽  
Vol 53 (4) ◽  
pp. 347-355 ◽  
Author(s):  
Frank Schnütgen ◽  
Franziska Ehrmann ◽  
Patricia Ruiz-Noppinger ◽  
Harald von Melchner
Keyword(s):  
High Throughput ◽  
Gene Trap ◽  
Gene Trapping

scholarly journals Loss of Cdc20 Causes a Securin-Dependent Metaphase Arrest in Two-Cell Mouse Embryos

2007 ◽  
Vol 27 (9) ◽  
pp. 3481-3488 ◽  
Author(s):  
Min Li ◽  
J. Philippe York ◽  
Pumin Zhang
Keyword(s):  
Ubiquitin Ligase ◽  
Cyclin B1 ◽  
Mitotic Exit ◽  
Adapter Proteins ◽  
Anaphase Promoting Complex ◽  
Metaphase Arrest ◽  
Cell Stage ◽  
Gene Trapping ◽  
Protein Substrates

ABSTRACT The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase mediating targeted proteolysis through ubiquitination of protein substrates to control the progression of mitosis. The APC/C recognizes its substrates through two adapter proteins, Cdc20 and Cdh1, which contain similar C-terminal domains composed of seven WD-40 repeats believed to be involved in interacting with their substrates. During the transition from metaphase to anaphase, APC/C-Cdc20 mediates the ubiquitination of securin and cyclin B1, allowing the activation of separase and the onset of anaphase and mitotic exit. APC/C-Cdc20 and APC/C-Cdh1 have overlapping substrates. It is unclear whether they are redundant for mitosis. Using a gene-trapping approach, we have obtained mice which lack Cdc20 function. These mice show failed embryogenesis. The embryos were arrested in metaphase at the two-cell stage with high levels of cyclin B1, indicating an essential role of Cdc20 in mitosis that is not redundant with that of Cdh1. Interestingly, Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit.

scholarly journals Gene trapping identifies chloride channel 4 as a novel inducer of colon cancer cell migration, invasion and metastases

2010 ◽  
Vol 102 (4) ◽  
pp. 774-782 ◽  
Author(s):  
T Ishiguro ◽  
H Avila ◽  
S-Y Lin ◽  
T Nakamura ◽  
M Yamamoto ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Chloride Channel ◽  
Colon Cancer Cell ◽  
Cancer Cell Migration ◽  
Gene Trapping

Transposon-mediated gene trapping in zebrafish

Methods ◽  
2006 ◽  
Vol 39 (3) ◽  
pp. 199-206 ◽  
Author(s):  
Tomoya Kotani ◽  
Saori Nagayoshi ◽  
Akihiro Urasaki ◽  
Koichi Kawakami
Keyword(s):  
Gene Trapping

Gene Trapping in Embryonic Stem Cells

2006 ◽  
pp. 136-162 ◽  
Author(s):  
William L. Stanford ◽  
Trevor Epp ◽  
Tammy Reid ◽  
Janet Rossant
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Gene Trapping
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