scholarly journals Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells

2005 ◽  
Vol 33 (2) ◽  
pp. e20-e20 ◽  
Author(s):  
T. Shigeoka
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mrna Decay ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trapping ◽  
Unbiased Gene ◽  
Nonsense Mediated Mrna Decay

scholarly journals MOV10 facilitates messenger RNA decay in an N6-methyladenosine (m6A) dependent manner to maintain the mouse embryonic stem cells state

2021 ◽  
Author(s):  
Majid Mehravar ◽  
Yogesh Kumar ◽  
Moshe Olshansky ◽  
Dhiru Bansal ◽  
Craig Dent ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Biology ◽  
Messenger Rna ◽  
Mrna Decay ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Beta Catenin ◽  
Dependent Manner ◽  
Leukaemia Virus

N6-methyladenosine (m6A) is the most predominant internal mRNA modification in eukaryotes, recognised by its reader proteins (so-called m6A-readers) for regulating subsequent mRNA fates, such as splicing, export, localisation, decay, stability, and translation to control several biological processes. Although a few m6A-readers have been identified, yet the list is incomplete. Here, we identify a new m6A-reader protein, Moloney leukaemia virus 10 homologue (MOV10), in mouse embryonic stem cells (mESCs). MOV10 recognises m6A-containing mRNAs with a conserved GGm6ACU motif. Mechanistic studies uncover that MOV10 facilitates mRNA decay of its bound m6A- containing mRNAs in an m6A-dependent manner within the cytoplasmic processing bodies (P-bodies). Furthermore, MOV10 decays the Gsk-3beta mRNA through m6A that stabilises the BETA-CATENIN expression of a WNT/BETA-CATENIN signalling pathway to regulate downstream NANOG expression for maintaining the mESC state. Thus, our findings reveal how a newly identified m6A-reader, MOV10 mediates mRNA decay via m6A that impact embryonic stem cell biology.

scholarly journals Large-scale gene trapping in C57BL/6N mouse embryonic stem cells

2008 ◽  
Vol 18 (10) ◽  
pp. 1670-1679 ◽  
Author(s):  
G. M. Hansen ◽  
D. C. Markesich ◽  
M. B. Burnett ◽  
Q. Zhu ◽  
K. M. Dionne ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Large Scale ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trapping

scholarly journals A novel view of the transcriptome revealed from gene trapping in mouse embryonic stem cells

2007 ◽  
Vol 17 (7) ◽  
pp. 1051-1060 ◽  
Author(s):  
G. Roma ◽  
G. Cobellis ◽  
P. Claudiani ◽  
F. Maione ◽  
P. Cruz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trapping

scholarly journals Enhanced gene trapping in mouse embryonic stem cells

2008 ◽  
Vol 36 (20) ◽  
pp. e133-e133 ◽  
Author(s):  
Frank Schnütgen ◽  
Jens Hansen ◽  
Silke De-Zolt ◽  
Carsten Horn ◽  
Marcus Lutz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trapping
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