scholarly journals Nuclear localization of ubiquitin-activating enzyme Uba1 is characterized in its mammalian temperature-sensitive mutant

2015 ◽  
Vol 20 (8) ◽  
pp. 659-666 ◽  
Author(s):  
Kimihiko Sugaya ◽  
Yoshie Ishihara ◽  
Sonoe Inoue
Keyword(s):  
Nuclear Localization ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

Induction of growth arrest by a temperature-sensitive p53 mutant is correlated with increased nuclear localization and decreased stability of the protein

1991 ◽  
Vol 11 (1) ◽  
pp. 582-585
Author(s):  
D Ginsberg ◽  
D Michael-Michalovitz ◽  
D Ginsberg ◽  
M Oren
Keyword(s):  
Cell Proliferation ◽  
Nuclear Localization ◽  
Growth Arrest ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Primary Fibroblasts ◽  
In Cells

A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.

scholarly journals Induction of growth arrest by a temperature-sensitive p53 mutant is correlated with increased nuclear localization and decreased stability of the protein.

1991 ◽  
Vol 11 (1) ◽  
pp. 582-585 ◽  
Author(s):  
D Ginsberg ◽  
D Michael-Michalovitz ◽  
D Ginsberg ◽  
M Oren
Keyword(s):  
Cell Proliferation ◽  
Nuclear Localization ◽  
Growth Arrest ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Primary Fibroblasts ◽  
In Cells

A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.

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