Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

2017 ◽  
Vol 73 (2) ◽  
pp. 368-380 ◽  
Author(s):  
J. P. P. Rodrigues ◽  
M. Ramin ◽  
P. Huhtanen ◽  
F. Aru ◽  
E. Detmann ◽  
...  
Keyword(s):  
Methane Production ◽  
Production System ◽  
Gas Production ◽  
Soya Bean ◽  
In Vitro Gas Production ◽  
Forage Type

Using krabok (Irvingia malayana) seed oil and Flemingia macrophylla leaf meal as a rumen enhancer in an in vitro gas production system

2017 ◽  
Vol 57 (2) ◽  
pp. 327 ◽  
Author(s):  
Sungchhang Kang ◽  
Metha Wanapat ◽  
Kampanat Phesatcha ◽  
Thitima Norrapoke ◽  
Suban Foiklang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Methane Production ◽  
Production System ◽  
Seed Oil ◽  
Gas Production ◽  
Flemingia Macrophylla ◽  
Production Kinetics ◽  
In Vitro Gas Production

An in vitro gas production system was conducted to investigate the effect of krabok (Irvingia malayana) seed oil (KSO) and Flemingia (Flemingia macrophylla) leaf powder (FLM) supplementation on gas production kinetics, volatile fatty acid (VFA) and methane production with different ratios of rice straw to cassava chip (RS : CC). The treatments were arranged according to a 4 × 2 × 2 factorial arrangement in a completely randomised design using four different ratios of RS : CC (100 : 0; 60 : 40; 20 : 80; and 0 : 100), two levels of KSO supplement (0% and 2.5% of total dietary substrate) and two levels of FLM supplement (0% and 2.5% of total dietary substrate). The gas production kinetics were affected by RS : CC, KSO and FLM supplementation (P < 0.05). However, there was no interaction between RS : CC*FLM, FLM*KSO, or RS : CC*FLM*KSO; with the exception of RS : CC*KSO (P < 0.05). Cumulative gas at 96 h post incubation was increased with increasing RS : CC especially at 0 : 100. However, KSO supplementation suppressed gas production whereas FLM could enhance gas production from feed fraction (P < 0.05). Increasing RS : CC ratio resulted in increasing total VFA, propionic acid and butyric acid whereas acetic acid concentration was reduced; therefore, ratio of acetic acid : propionic acid was reduced. However, KSO supplementation depressed VFA production whereas the FLM supplement had no effect. The methane production was reduced with increasing level of RS : CC especially with supplementation of KSO. Based on this study, it is concluded that KSO addition could reduce methane production whereas FLM could enhance the gas production and fermentation end products; hence, the combined use is potentially beneficial. However, further research under in vivo conditions should be conducted.

332 Effect of Chemical and Biological Preservatives on in vitro Fermentation and Methane Production of Ensiled and Aerobically Exposed Wet Brewer’s Grains

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 182-182
Author(s):  
Marjorie A Killerby ◽  
Diego Zamudio ◽  
Kaycee Ames ◽  
Darren D Henry ◽  
Thomas Schwartz ◽  
...  
Keyword(s):  
Methane Production ◽  
Production System ◽  
Methane Concentration ◽  
Block Design ◽  
Gas Production ◽  
Sodium Lignosulfonate ◽  
In Vitro Fermentation ◽  
Randomized Complete Block Design ◽  
System Data

Abstract This study evaluated the effects of preservatives on the in vitro fermentation measures of wet brewer’s grain (WBG) silage at different stages of storage. Treatments (TRT) were sodium lignosulfonate at 1% (NaL1) and 2% (NaL2; w/w of fresh WBG), propionic acid (PRP; 0.5% w/w of fresh WBG), a combination inoculant (INO; Lactococcus lactis and Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated WBG (CON). WBG (Fresh) were packed into 8.8 L mini-silos and stored for 60 d at 21°C (Ensiled), then they were opened and aerobically exposed for 10d (AES). Samples from each stage of storage (STG; Fresh, Ensiled and AES) were analyzed for in vitro ruminal digestibility (24 h).Gas kinetics were recorded using the Ankom RF Gas Production System. Data were analyzed as a randomized complete block design (5 blocks) with a 5 (TRT) × 3 (STG) factorial arrangement. Apparent in vitro DM digestibility (DMD) decreased across STG, (51.5, 47.2 and 40.9 for Fresh, Ensiled and AES, respectively) and increased for NaL1, NaL2 and PRP (~47.8) vs. CON (43.0 ± 2.12%). PRP increased apparent in vitro OM digestibility (OMD) when Ensiled (54.5) and NaL2 increased it for AES (47.1) vs CON (46.3 and 39.9 ± 1.73%, respectively). The asymptotic maximal (M) and rate (k) of gas production decreased across STG (214.6, 181.5, 155.1 and 14.6, 12.6, and 9.8, for Fresh, Ensiled and AES, respectively). PRP increased (200.0) and NaL1 decreased (169.3) M vs. CON (183.9± 7.81ml/incubated DM g), while NaL1 and NaL2 (~11.4) decreased k vs. CON (13.4 ± 0.85%/h). Methane concentration and yield were higher in Fresh vs. other STG (0.94 vs. ~0.84 ± 0.07mM and 0.27 vs. ~0.23 ± 0.03mmol/g fermented OM). Spoilage of WBG decreases fermentability and methane production while PRP and NaL improve digestibility with the former also increasing M and k.

scholarly journals Silage of Intercropping Corn, Palisade Grass, and Pigeon Pea Increases Protein Content and Reduces In Vitro Methane Production

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1784
Author(s):  
Beatriz Ligoski ◽  
Lucas Ferreira Gonçalves ◽  
Flavio Lopes Claudio ◽  
Estenio Moreira Alves ◽  
Ana Maria Krüger ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Nutritional Quality ◽  
Production Systems ◽  
Animal Feed ◽  
Lignin Content ◽  
Pigeon Pea ◽  
Gas Production ◽  
In Vitro Gas Production

Legume–grass intercropping systems are a sustainable option to improve nutritional quality of animal feed and decrease livestock greenhouse gas emissions. Thus, the present study evaluated yield, chemical composition and in vitro gas production of silages produced with intercropped palisade grass (Urochloa brizantha.(A.Rich.) R.D.Webster), pigeon pea (Cajanus cajan cv. Super N) and corn (Zea mays. L.). Forage was harvested and placed inside micro-silos, which were opened after 100 days and samples were collected for chemical composition and in vitro gas production analyses. Intercropped silage had higher crude protein, acid detergent fiber, and lignin content than corn silage. Moreover, intercropped silage decreased total gas and methane production. Therefore, intercropped silage showed potential to increase conserved feed nutritional quality and reduce methane emissions in livestock production systems.

Modelling fermentation in an in vitro gas production system: effects of microbial activity

1998 ◽  
Vol 22 ◽  
pp. 81-84 ◽  
Author(s):  
N. S. Jessop ◽  
M. Herrero
Keyword(s):  
Microbial Activity ◽  
Production System ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Food Fermentation ◽  
In Vitro Gas Production ◽  
And Performance ◽  
Physical And Chemical

In order to understand and ultimately predict the voluntary intake and performance of ruminants, it is necessary to know the nutritional value of foods. Most recent systems for predicting nutrient supply are dynamic in nature and characterize foods in terms of the quantities of available nutrients and their potential rates of supply. The in vitro gas production system has been used to characterize the carbohydrate fraction of foods in this manner. For the technique to be able to do this, two assumptions must be satisfied. First, that the rate of fermentation is limited by characteristics of the food and secondly that the pattern of gas production correlates closely with the pattern of food fermentation.Low microbial activity within the system could invalidate both assumptions since it could (i) limit the rate of food fermentation, thus not allowing the potential rate determined by the physical and chemical nature of the food to be measured and (ii) result in partition of food carbohydrate into new microbial matter, thus reducing the amount of volatile fatty acids and hence gas produced per unit of food fermented.The aims of this study were mathematically to simulate food fermentation within an in vitro system and to use this representation to investigate the potential effects of variation in microbial activity on the characterization of foods.

scholarly journals A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment

2011 ◽  
Vol 168 (3-4) ◽  
pp. 196-205 ◽  
Author(s):  
W.F. Pellikaan ◽  
W.H. Hendriks ◽  
G. Uwimana ◽  
L.J.G.M. Bongers ◽  
P.M. Becker ◽  
...  
Keyword(s):  
Methane Production ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Novel Method

Effect of Sulfate-Containing Compounds on Methane Production by Using an In vitro Gas Production Technique

2013 ◽  
Vol 12 (8) ◽  
pp. 723-729 ◽  
Author(s):  
Pattaya Napasirth ◽  
Chalong Wachirapak ◽  
Pathcharee Saenjan ◽  
Chalermpon Yuangklang
Keyword(s):  
Methane Production ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

scholarly journals Condition of Rumen Fermentation as Impacted by Supplementation of Fermented Rice Brand Using In Vitro Gas Production Technique

2020 ◽  
Vol 25 (2) ◽  
pp. 74
Author(s):  
Zuratih Zuratih ◽  
SPS Budhi ◽  
Z Bachruddin
Keyword(s):  
Rice Bran ◽  
Methane Production ◽  
Basal Diet ◽  
Gas Production ◽  
Monascus Purpureus ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Monacolin K ◽  
In Vitro Gas Production

Methane is one of the gases produced by ruminants during feed fermentation in the rumen. This experiment was aimed to investigate the production of monacolin K in rice bran fermented by Monascus purpureus mold and the influence of the supplementation of fermented rice bran using Monascus purpureus mold on elephant grass basal diet on fermentation products and methane production in an in vitro gas production method. The study consisted of two experiments. The first experiment analysis of monacolin K production in fermented rice bran using Monascus purpureus. Fermentation is done by the addition of Monascus purpureus at levels 0, 4, 8, and 12% (v/w) of substrate (rice bran) with 3 replications. Monacolin K in the substrate was analyzed using HPLC. The second experiment was the evaluation of supplementation of fermented rice bran to elephant grass basal diet using in vitro gas production. The treatment diet evaluated were Pennisetum purpureum (control), Pennisetum purpureum:rice bran (1:1 ratio), and Pennisetum purpureum:rice bran fermented. Each treatment was replicated 3 times. Results from the first experiment shows that rice bran with the highest monacolin K content was in rice bran fermented at 12% by Monascus purpureus. Result from the second experiment showed that supplementation of fermented rice bran to Pennisetum purpureum basal diet did not affect rumen ammonia concentration, VFA, protein microbial production, and dry matter and organic matter digestibility. However, methane production (CH<sub>4</sub>) was reduced (P&lt;0.05) by 50%, and the protozoal population was decreased (P&lt;0.05) by 80%. It is concluded that supplementation of fermented rice brands containing monacolin K was able to reduce methane production and the protozoa population without affecting feed fermentation.

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