Protein regulator of cytokinesis 1 regulates chromosome dynamics and cytoplasmic division during mouse oocyte meiotic maturation and early embryonic development

FEBS Journal ◽  
2020 ◽  
Vol 287 (23) ◽  
pp. 5130-5147
Author(s):  
Cheng‐Jie Zhou ◽  
Dong‐Hui Wang ◽  
Xiang‐Wei Kong ◽  
Zhe Han ◽  
Xin Hao ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Mouse Oocyte ◽  
Early Embryonic Development ◽  
Meiotic Maturation ◽  
Chromosome Dynamics ◽  
Oocyte Meiotic Maturation

200 SYNCHRONIZATION OF OOCYTE MEIOTIC MATURATION IN SUPEROVULATED MICE IMPROVES IN VITRO FERTILIZATION RATE

2012 ◽  
Vol 24 (1) ◽  
pp. 212
Author(s):  
A. M. Taiyeb Ridha ◽  
D. C. Kraemer
Keyword(s):  
Embryonic Development ◽  
Fertilization Rate ◽  
Early Embryonic Development ◽  
Meiotic Maturation ◽  
Control Group ◽  
Vitro Fertilization ◽  
Development Rates ◽  
Oocyte Meiotic Maturation

In vitro synchronization of oocyte nuclear and cytoplasmic maturation has been found to improve the IVF rate of ovarian oocytes in several species, including humans, in comparison with nonsynchronized in vitro-matured oocytes. Here, we tested the hypothesis that synchronization of oocyte meiotic maturation by an in vivo system in superovulated mice would increase the oocyte fertilization rate when compared to that of conventional superovulated oocytes. Recently, we observed that cilostazol (CZL), a PDE3-I, was able to inhibit mouse oocyte meiotic maturation in both in vitro and in vivo systems. Administering CZL at 7.5 mg, 4 or 7 h pre-hCG allowed retrieval of ovulated oocytes of which >95% were at MI stage, scored by Nikon stereo microscope (SMZ 1500). A conventional superovulation program was adapted in all treated and their control groups, in which mice were injected with eCG and after 48 h with hCG (7.5 IU for each hormone). On the second morning, 13 to 14 h post-hCG, mice were killed and oocytes were collected from oviducts and in vitro fertilized (control). For the treated groups, CZL was administered in a single 7.5 mg oral dose (gavage) 4 or 7 h before the hCG injection. On the second morning, CZL-treated animals were killed at the same timing as control animals and oocytes were retrieved from the oviduct and in vitro matured for 6 h (for those gavaged with CZL, 4 h pre-hCG) or 3 h (for those gavaged with CZL, 7 h pre-hCG) to MII oocytes before IVF. These groups were designated as in vivo-in vitro synchronized/matured oocytes. In other groups treated with CZL, 4 or 7 h pre-hCG, the ovulated oocytes were allowed to mature in the oviduct (full in vivo synchronization and maturation) and oocytes were retrieved and fertilized with the same fertilization timings as the in vivo-in vitro synchronized/matured oocytes. Oocytes were cultured for 1 day after IVF and examined for cleavage. Statistical differences were analyzed by cross-tabulated chi-square test. The full in vivo synchronization and maturation (for both CZL dose timings of 4 and 7 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [89% (n = 219) and 92.2% (n = 374) vs 81.8% (n = 198); P = 0.034 and P < 0.0001, respectively]. The in vivo-in vitro synchronized/matured oocytes (CZL dose timing at 7 h, but not 4 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [88.5% (n = 339) vs 83.4% (n = 458), respectively; P = 0.043]. However, the increase of the IVF rate of the oocytes from mice treated with CZL, 4 h pre-hCG, in the in vivo-in vitro synchronized/matured group was not significantly different from the control group [88.5% (n = 399) vs 83.4% (n = 458), respectively; P = 0.43]. It is concluded from the present study that synchronization of oocyte meiotic maturation by the in vivo and in vivo-in-vitro protocols can increase the IVF rate of oocytes in superovulated mice.

scholarly journals Loss of function of KIF1B impairs oocyte meiotic maturation and early embryonic development in mice

2016 ◽  
Vol 83 (11) ◽  
pp. 1027-1040 ◽  
Author(s):  
Xiang-Wei Kong ◽  
Dong-Hui Wang ◽  
Cheng-Jie Zhou ◽  
Hong-Xia Zhou ◽  
Cheng-Guang Liang
Keyword(s):  
Embryonic Development ◽  
Early Embryonic Development ◽  
Meiotic Maturation ◽  
Loss Of Function ◽  
Oocyte Meiotic Maturation

scholarly journals Maternal mRNAs with distinct 3′ UTRs define the temporal pattern ofCcnb1synthesis during mouse oocyte meiotic maturation

2017 ◽  
Vol 31 (13) ◽  
pp. 1302-1307 ◽  
Author(s):  
Ye Yang ◽  
Cai-Rong Yang ◽  
Seung Jin Han ◽  
Enrico Maria Daldello ◽  
Ara Cho ◽  
...  
Keyword(s):  
Temporal Pattern ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maternal Mrnas ◽  
Oocyte Meiotic Maturation

Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126182 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
Muhammad Safdar ◽  
Fan Chen ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

2011 ◽  
Vol 17 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Xin Huang ◽  
Jing-Shan Tong ◽  
Zhen-Bo Wang ◽  
Cai-Rong Yang ◽  
Shu-Tao Qi ◽  
...  
Keyword(s):  
Polar Body ◽  
Specific Inhibitor ◽  
Germinal Vesicle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Cytoplasmic Microtubule ◽  
Spindle Poles ◽  
First Polar Body ◽  
Oocyte Meiotic Maturation

AbstractIt is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.

Global Analysis of Maternal mRNA Translation during Mouse Oocyte Meiotic Maturation.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 327-327
Author(s):  
Jing Chen ◽  
Collin Melton ◽  
NaYoung Suh ◽  
Robert Blelloch ◽  
Marco Conti
Keyword(s):  
Global Analysis ◽  
Mrna Translation ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maternal Mrna ◽  
Oocyte Meiotic Maturation

scholarly journals Mitofusin-2 is required for mouse oocyte meiotic maturation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jing-Hua Zhang ◽  
Teng Zhang ◽  
Si-Hua Gao ◽  
Ke Wang ◽  
Xiu-Yan Yang ◽  
...  
Keyword(s):  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Mitofusin 2 ◽  
Oocyte Meiotic Maturation
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