scholarly journals The transcription factor Pf-POU3F4 regulates expression of the matrix protein genesAspeinandPrismalin-14in pearl oyster (Pinctada fucata)

FEBS Journal ◽  
2016 ◽  
Vol 283 (10) ◽  
pp. 1962-1978 ◽  
Author(s):  
Jing Gao ◽  
Yan Chen ◽  
Yi Yang ◽  
Jian Liang ◽  
Jun Xie ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
The Matrix

scholarly journals Transcriptional regulation of the matrix protein Shematrin-2 during shell formation in pearl oyster

2018 ◽  
Vol 293 (46) ◽  
pp. 17803-17816 ◽  
Author(s):  
Yan Chen ◽  
Jing Gao ◽  
Jun Xie ◽  
Jian Liang ◽  
Guilan Zheng ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Calcium Carbonate ◽  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Functional Assay ◽  
Shell Formation ◽  
Molluscan Shell ◽  
The Matrix

The molluscan shell is a fascinating biomineral consisting of a highly organized calcium carbonate composite. Biomineralization is elaborately controlled and involves several macromolecules, especially matrix proteins, but little is known about the regulatory mechanisms. The matrix protein Shematrin-2, expression of which peaks in the mantle tissues and in the shell components of the pearl oyster Pinctada fucata, has been suggested to be a key participant in biomineralization. Here, we expressed and purified Shematrin-2 from P. fucata and explored its function and transcriptional regulation. An in vitro functional assay revealed that Shematrin-2 binds the calcite, aragonite, and chitin components of the shell, decreases the rate of calcium carbonate deposition, and changes the morphology of the deposited crystal in the calcite crystallization system. Furthermore, we cloned the Shematrin-2 gene promoter, and analysis of its sequence revealed putative binding sites for the transcription factors CCAAT enhancer–binding proteins (Pf-C/EBPs) and nuclear factor-Y (NF-Y). Using transient co-transfection and reporter gene assays, we found that cloned and recombinantly expressed Pf-C/EBP-A and Pf-C/EBP-B greatly and dose-dependently up-regulate the promoter activity of the Shematrin-2 gene. Importantly, Pf-C/EBP-A and Pf-C/EBP-B knockdowns decreased Shematrin-2 gene expression and induced changes in the inner-surface structures in prismatic layers that were similar to those of antibody-based Shematrin-2 inhibition. Altogether, our data reveal that the transcription factors Pf-C/EBP-A and Pf-C/EBP-B up-regulate the expression of the matrix protein Shematrin-2 during shell formation in P. fucata, improving our understanding of the transcriptional regulation of molluscan shell development at the molecular level.

A novel matrix protein participating in the nacre framework formation of pearl oyster, Pinctada fucata

2003 ◽  
Vol 135 (3) ◽  
pp. 565-573 ◽  
Author(s):  
Yong Zhang ◽  
Liping Xie ◽  
Qingxiong Meng ◽  
Tiemin Jiang ◽  
Ruolei Pu ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata

Molecular cloning and characterization of class I NF-κB transcription factor from pearl oyster (Pinctada fucata)

2012 ◽  
Vol 33 (3) ◽  
pp. 659-666 ◽  
Author(s):  
Xian-De Huang ◽  
Wen-Guang Liu ◽  
Yun-Yan Guan ◽  
Yu Shi ◽  
Qi Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Cloning ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Class I

scholarly journals Identification and characterization of a matrix protein (PPP-10) in the periostracum of the pearl oyster, Pinctada fucata

FEBS Open Bio ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 421-427 ◽  
Author(s):  
Seiji Nakayama ◽  
Michio Suzuki ◽  
Hirotoshi Endo ◽  
Kurin Iimura ◽  
Shigeharu Kinoshita ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Identification And Characterization
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