A novel matrix protein family participating in the prismatic layer framework formation of pearl oyster, Pinctada fucata

2006 ◽  
Vol 344 (3) ◽  
pp. 735-740 ◽  
Author(s):  
Cen Zhang ◽  
Liping Xie ◽  
Jing Huang ◽  
Xiaolei Liu ◽  
Rongqing Zhang
Keyword(s):  
Matrix Protein ◽  
Pearl Oyster ◽  
Protein Family ◽  
Pinctada Fucata ◽  
Prismatic Layer

scholarly journals Characterization of Prismalin-14, a novel matrix protein from the prismatic layer of the Japanese pearl oyster (Pinctada fucata)

2004 ◽  
Vol 382 (1) ◽  
pp. 205-213 ◽  
Author(s):  
Michio SUZUKI ◽  
Emi MURAYAMA ◽  
Hirotaka INOUE ◽  
Noriaki OZAKI ◽  
Hidekazu TOHSE ◽  
...  
Keyword(s):  
Amino Acid ◽  
Calcium Carbonate ◽  
Amino Acid Sequence ◽  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Intact Protein ◽  
Prismatic Layer ◽  
Organic Matrices

The mollusc shell is a hard tissue consisting of calcium carbonate and organic matrices. The organic matrices are believed to play important roles in shell formation. In the present study, we extracted and purified a novel matrix protein, named Prismalin-14, from the acid-insoluble fraction of the prismatic layer of the shell of the Japanese pearl oyster (Pinctada fucata), and determined its whole amino acid sequence by a combination of amino acid sequence analysis and MS analysis of the intact protein and its enzymic digests. Prismalin-14 consisted of 105 amino acid residues, including PIYR repeats, a Gly/Tyr-rich region and N- and C-terminal Asp-rich regions. Prismalin-14 showed inhibitory activity on calcium carbonate precipitation and calcium-binding activity in vitro. The scanning electron microscopy images revealed that Prismalin-14 affected the crystallization of calcium carbonate in vitro. A cDNA encoding Prismalin-14 was cloned and its expression was analysed. The amino acid sequence deduced from the nucleotide sequence of Prismalin-14 cDNA was identical with that determined by peptide sequencing. Northern-blot analysis showed that a Prismalin-14 mRNA was expressed only at the mantle edge. In situ hybridization demonstrated that a Prismalin-14 mRNA was expressed strongly in the inner side of the outer fold of the mantle. These results suggest that Prismalin-14 is a framework protein that plays an important role in the regulation of calcification of the prismatic layer of the shell.

A novel matrix protein participating in the nacre framework formation of pearl oyster, Pinctada fucata

2003 ◽  
Vol 135 (3) ◽  
pp. 565-573 ◽  
Author(s):  
Yong Zhang ◽  
Liping Xie ◽  
Qingxiong Meng ◽  
Tiemin Jiang ◽  
Ruolei Pu ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata

scholarly journals The transcription factor Pf-POU3F4 regulates expression of the matrix protein genesAspeinandPrismalin-14in pearl oyster (Pinctada fucata)

FEBS Journal ◽  
2016 ◽  
Vol 283 (10) ◽  
pp. 1962-1978 ◽  
Author(s):  
Jing Gao ◽  
Yan Chen ◽  
Yi Yang ◽  
Jian Liang ◽  
Jun Xie ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
The Matrix

scholarly journals A new matrix protein family related to the nacreous layer formation of Pinctada fucata

FEBS Letters ◽  
1999 ◽  
Vol 462 (1-2) ◽  
pp. 225-229 ◽  
Author(s):  
Tetsuro Samata ◽  
Nakanobu Hayashi ◽  
Makiko Kono ◽  
Kyoko Hasegawa ◽  
Chie Horita ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Protein Family ◽  
Pinctada Fucata ◽  
Nacreous Layer ◽  
Layer Formation

Characteristics of biogenic calcite in the prismatic layer of a pearl oyster, Pinctada fucata

Micron ◽  
2010 ◽  
Vol 41 (7) ◽  
pp. 821-826 ◽  
Author(s):  
Taiga Okumura ◽  
Michio Suzuki ◽  
Hiromichi Nagasawa ◽  
Toshihiro Kogure
Keyword(s):  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Prismatic Layer
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