The alteration of the C-terminal region of human frataxin distorts its structural dynamics and function

FEBS Journal ◽  
2014 ◽  
Vol 281 (15) ◽  
pp. 3397-3419 ◽  
Author(s):  
Santiago E. Faraj ◽  
Ernesto A. Roman ◽  
Martin Aran ◽  
Mariana Gallo ◽  
Javier Santos
Keyword(s):  
Structural Dynamics ◽  
Terminal Region ◽  
Dynamics And Function ◽  
And Function

scholarly journals Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments

2007 ◽  
Vol 313 (10) ◽  
pp. 2098-2109 ◽  
Author(s):  
Ram K. Sihag ◽  
Masaki Inagaki ◽  
Tomoya Yamaguchi ◽  
Thomas B. Shea ◽  
Harish C. Pant
Keyword(s):  
Structural Dynamics ◽  
Intermediate Filaments ◽  
Dynamics And Function ◽  
And Function

scholarly journals NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mengjie Sun ◽  
Mingkang Jia ◽  
He Ren ◽  
Biying Yang ◽  
Wangfei Chi ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Structural Dynamics ◽  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Spindle Poles ◽  
Mitotic Spindle Assembly ◽  
Dynamics And Function ◽  
And Function ◽  
Liquid Liquid Phase Separation

AbstractA functional mitotic spindle is essential for accurate chromosome congression and segregation during cell proliferation; however, the underlying mechanisms of its assembly remain unclear. Here we show that NuMA regulates this assembly process via phase separation regulated by Aurora A. NuMA undergoes liquid-liquid phase separation during mitotic entry and KifC1 facilitates NuMA condensates concentrating on spindle poles. Phase separation of NuMA is mediated by its C-terminus, whereas its dynein-dynactin binding motif also facilitates this process. Phase-separated NuMA droplets concentrate tubulins, bind microtubules, and enrich crucial regulators, including Kif2A, at the spindle poles, which then depolymerizes spindle microtubules and promotes poleward spindle microtubule flux for spindle assembly and structural dynamics. In this work, we show that NuMA orchestrates mitotic spindle assembly, structural dynamics and function via liquid-liquid phase separation regulated by Aurora A phosphorylation.

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