scholarly journals The excluded volume effect induced by poly(ethylene glycol) modulates the motility of actin filaments interacting with myosin

FEBS Journal ◽  
2013 ◽  
Vol 280 (22) ◽  
pp. 5875-5883 ◽  
Author(s):  
Shinsuke Munakata ◽  
Kuniyuki Hatori
Keyword(s):  
Ethylene Glycol ◽  
Actin Filaments ◽  
Volume Effect ◽  
Excluded Volume ◽  
Poly Ethylene Glycol ◽  
Excluded Volume Effect ◽  
Poly Ethylene

scholarly journals The control of cellular shape and motility. Mg2+ and tropomyosin regulate the formation and the dissociation of microfilament bundles

1992 ◽  
Vol 288 (3) ◽  
pp. 727-732 ◽  
Author(s):  
E Grazi ◽  
P Cuneo ◽  
A Cataldi
Keyword(s):  
Ethylene Glycol ◽  
Actin Filaments ◽  
Adenine Nucleotide ◽  
Energy Charge ◽  
Poly Ethylene Glycol ◽  
Microfilament Bundles ◽  
Filament Bundles ◽  
Poly Ethylene

At pH 7.14 and 37 degrees C, in 7.2% (w/v) poly(ethylene glycol) 6000, tropomyosin-regulated actin filaments are converted into filament bundles by increasing the free Mg2+ concentration to 1.7-2.0 mM. When free Mg2+ concentration is decreased below 1.7 mM, bundles dissociate back into tropomyosin-regulated actin filaments. Pure actin filaments are insensitive to this mechanism of control and are found as filament bundles in all the range of free Mg2+ concentrations tested (1.37-2.2 mM). The mechanism of regulation described above is likely to operate in the cell, where the concentration of free Mg2+ is linked to the energy charge of the adenine nucleotide system.

scholarly journals ‘Macromolecular crowding’ is a primary factor in the organization of the cytoskeleton

1992 ◽  
Vol 281 (2) ◽  
pp. 507-512 ◽  
Author(s):  
P Cuneo ◽  
E Magri ◽  
A Verzola ◽  
E Grazi
Keyword(s):  
Ethylene Glycol ◽  
Actin Filaments ◽  
Macromolecular Crowding ◽  
Cytoskeletal Proteins ◽  
Primary Factor ◽  
Poly Ethylene Glycol ◽  
Experimental Conditions ◽  
Actin Bundles ◽  
Poly Ethylene

We propose that, in the cell, the reversible conversion of actin filaments into actin bundles is controlled by the concentration of the macromolecules [we have employed poly(ethylene glycol) 6000 to mimic the macromolecules of the cell] as well as by the nature of the ancillary cytoskeletal proteins that decorate actin filaments. The proposal is based on the following evidence. (1) Under our experimental conditions the transition from filaments into bundles occurs at increasing concentrations of poly(ethylene glycol), with the following sequence: caldesmon-actin, 3%; filamin-actin, 4-5%; caldesmon-tropomyosin-actin, 5-7%; actin, 6-7%; tropomyosin-actin, 9-10%. (2) Under conditions of low osmoelastic stress [3% poly(ethylene glycol)], preformed caldesmon-actin bundles are dissociated by the addition of either tropomyosin or tropomyosin-decorated actin. The dissociation of the bundles promoted by the addition of tropomyosin-decorated actin is faster than that promoted by the addition of tropomyosin.

scholarly journals The influence of poly(ethylene glycol) 6000 on the properties of skeletal-muscle actin

1983 ◽  
Vol 213 (3) ◽  
pp. 651-659 ◽  
Author(s):  
R L Tellam ◽  
M J Sculley ◽  
L W Nichol ◽  
P R Wills
Keyword(s):  
Skeletal Muscle ◽  
Ethylene Glycol ◽  
Actin Polymerization ◽  
Excluded Volume ◽  
Muscle Actin ◽  
Actin Monomer ◽  
Poly Ethylene Glycol ◽  
Fluorescence Measurements ◽  
Volume Increment ◽  
Poly Ethylene

Poly(ethylene glycol) 6000 affected many of the properties of skeletal-muscle actin. It accelerated the rate and increased the extent of actin polymerization as measured by light-scattering and sedimentation studies respectively. Moreover, intrinsic-fluorescence measurements showed that addition of poly(ethylene glycol) 6000 decreased the rate of EDTA-induced denaturation of actin monomer and increased the temperature at which irreversible conformational changes occur in actin monomer. These effects occurred without any apparent direct binding interaction and are postulated to be a consequence of the effect of excluded volume on the thermodynamic activity of actin. A relationship based on spherical geometry was formulated which described the co-volume increment that occurs upon addition of a monomer to a long linear polymer in the presence of a space-filling macromolecule. The application of this relationship to the poly(ethylene glycol) 6000-actin system was not without assumption, but it permitted quantitative estimation of the co-volume increment which proved to be of the sign and magnitude required to explain the increased extent of actin polymerization found experimentally in the presence of various concentrations of poly(ethylene glycol) 6000. It is suggested that, in vivo, excluded volume may play a role in actin-filament formation and in the maintenance of the native G-actin structure.

Electrostatic and Excluded Volume Effects on the Transport of Electrolytes in Poly(ethylene glycol)−Water “Mixed Solvents”

2003 ◽  
Vol 107 (44) ◽  
pp. 12363-12369 ◽  
Author(s):  
Fabio Capuano ◽  
Alessandro Vergara ◽  
Luigi Paduano ◽  
Onofrio Annunziata ◽  
Roberto Sartorio
Keyword(s):  
Ethylene Glycol ◽  
Mixed Solvents ◽  
Excluded Volume ◽  
Poly Ethylene Glycol ◽  
Excluded Volume Effects ◽  
Poly Ethylene ◽  
Volume Effects

PIEZOELECTRIC PROPERTIES OF POLY(3-HYDROXYBUTYRATE-CO-3-HEDROXYBUTYRATE)-CO-POLY(ETHYLENE GLYCOL) PRODUCED BY CONTROLLED MICROBIOLOGICAL BIOSYNTHESIS

2019 ◽  
Vol 10 (10) ◽  
Author(s):  
Anton Bonartsev ◽  
Vera Voinova ◽  
Elizaveta Akoulina ◽  
Andrey Dudun ◽  
Irina Zharkova ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Piezoelectric Properties ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Thermosensitives hydrogels based on poly (ethylene glycol): I. Synthesis, characterization and release

2007 ◽  
Vol 32 (5) ◽  
pp. 431-446 ◽  
Author(s):  
Tahar Bartil ◽  
Mahmoud Bounekhel ◽  
Cedric Calberg ◽  
Robert Jerome
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Quantifying Heart Valve Interstitial Cell Contractile State Using Highly Tunable Poly(Ethylene Glycol) Hydrogels

2019 ◽  
Author(s):  
Alex Khang ◽  
Andrea Gonzalez Rodriguez ◽  
Megan E. Schroeder ◽  
Jacob Sansom ◽  
Emma Lejeune ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Heart Valve ◽  
Interstitial Cell ◽  
Poly Ethylene Glycol ◽  
Contractile State ◽  
Poly Ethylene
Sign in / Sign up

Export Citation Format

Share Document