scholarly journals Re‐innervation of human skin by rat dorsal root ganglia permits to study interactions between sensory nerve fibres and native human dermal mast cells ex vivo

2020 ◽  
Author(s):  
Jérémy Chéret ◽  
Leslie Ponce ◽  
Christelle Le Gall‐Ianotto ◽  
Marta Bertolini ◽  
RALF PAUS
Keyword(s):  
Mast Cells ◽  
Human Skin ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Ex Vivo ◽  
Sensory Nerve ◽  
Nerve Fibres

scholarly journals Aged human skin accumulates mast cells with altered functionality that localize to macrophages and vasoactive intestinal peptide‐positive nerve fibres

2018 ◽  
Vol 180 (4) ◽  
pp. 849-858 ◽  
Author(s):  
S.M. Pilkington ◽  
M.J. Barron ◽  
R.E.B. Watson ◽  
C.E.M. Griffiths ◽  
S. Bulfone‐Paus
Keyword(s):  
Mast Cells ◽  
Vasoactive Intestinal Peptide ◽  
Human Skin ◽  
Nerve Fibres

Selective Reinnervation of Regenerating Mixed Nerve Fibres Across a Silicone Tube Gap

1996 ◽  
Vol 21 (5) ◽  
pp. 660-663 ◽  
Author(s):  
Z-J. LUO ◽  
S-B. LU
Keyword(s):  
Dorsal Root ◽  
Tibial Nerve ◽  
Motor Nerve ◽  
Sensory Nerve ◽  
Nerve Fibres ◽  
Silicone Tube ◽  
Spinal Dorsal ◽  
Sensory Axons ◽  
Target Organs ◽  
Mixed Nerve

This study investigated specific regeneration of a mixed motor and sensory nerve by the method of spinal dorsal root ganglions resection. A 10 mm segment of tibial nerve was resected and the nerve ends inserted in a silicone tube. Fourteen weeks later, dorsal root ganglia from L6 to S1 were resected on the experiment side. Twenty weeks later, the regenerating motor nerve fibres of mixed nerves selectively grew into motor branches. The rate of misdirected growth in mixed nerves was less than 6%. These results suggest that regenerating motor and sensory axons of mixed nerves are able to select their distal target organs accurately. Better results may be obtained using the entubulation repair method.

The origin and possible significance of substance P immunoreactive networks in the prevertebral ganglia and related structures in the guinea-pig

1984 ◽  
Vol 306 (1128) ◽  
pp. 247-276 ◽  
Keyword(s):  
Substance P ◽  
Sensory Neurons ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nerve Fibres ◽  
Inferior Mesenteric Ganglion ◽  
Immunoreactive Material ◽  
Splanchnic Nerves ◽  
Prevertebral Ganglia ◽  
Mesenteric Ganglion

The distribution and origin of substance P immunoreactive nerve elements have been studied in the guinea-pig prevertebral ganglia by the indirect immunohistochemical technique, using a monoclonal antibody to substance P. Non-varicose substance P immunoreactive nerve fibres enter or leave the ganglia in all nerves associated with them, traversing the ganglia in larger or smaller bundles. Networks, mainly singlestranded, of varicose substance P immunoreactive nerve fibres also permeate the ganglia, forming a loose meshwork among the neurons. Similar networks are present in the lumbar paravertebral ganglia. In all these ganglia, neuronal somata do not in general show substance P immunoreactivity. The various nerves connected with the inferior mesenteric ganglion have been cut, in single categories and in various combinations, and the ganglion examined, after intervals of up to six days. Cutting the colonic or hypogastric nerves, which connect the ganglion with the hindgut and pelvic organs, leads to accumulation of substance P immunoreactive material in their ganglionic stumps, extending retrogradely to intraganglionic non-varicose fibres traceable through into the intermesenteric and lumbar splanchnic nerves. There is some local depletion of intraganglionic varicose networks. Cutting the intermesenteric nerve, which connects the coeliac-superior mesenteric ganglion complex with the ganglion, leads to accumulation of substance P immunoreactive material in its cranial stump and depletion of its distal stump; a minimal depletion is detectable in the inferior mesenteric ganglion itself. Cutting the lumbar splanchnic nerves, which connect the ganglion with the upper lumbar spinal cord and dorsal root ganglia, leads to accumulation of substance P immunoreactive material in their proximal stumps and total depletion of their distal, ganglionic stumps; in the ganglion there is subtotal loss of non-varicose substance P immunoreactive fibres and of varicose nerve networks, and the few surviving non-varicose fibres are traceable across the ganglion from the intermesenteric nerve to the colonic and hypogastric nerves. Cutting the intermesenteric and lumbar splanchnic nerves virtually abolishes substance P immunoreactive elements from the ganglion within three days postoperatively. It is concluded that these arise centrally to the ganglion. Capsaicin treatm ent of guinea-pigs, which depletes substance P immunoreactivity of sensory neurons, was found to leave no more than minute occasional traces of substance P immunoreactivity in the prevertebral ganglia and in dorsal root ganglion cells and spinal laminae I and II; in the ileum, substance P immunoreactivity was abolished from the paravascular nerves and perivascular nerve networks, and from large nerve varicosities in the submucous plexus. The substance P immunoreactivity of the myenteric and submucous plexuses and of the nerve networks in the muscle and mucosal layers was however otherwise unaffected. Removal of the spinal cord caudally to the seventh thoracic segment without injury to the dorsal root ganglia is without detectable effect either on the substance P immunoreactive elements of the inferior mesenteric ganglion and associated nerves, or on the peri- and paravascular nerves of the hindgut mesenteric vessels. It is concluded that the intraganglionic substance P immunoreactive elements of the prevertebral ganglia are attributable to sensory neurons of the dorsal root ganglia, and that intraspinal and enteric neurons do not contribute significantly to them. It is further postulated with the support of indirect evidence that the intraganglionic networks of varicose substance P immunoreactive fibres, which have been shown to form synapses upon the postganglionic neurons, arise as collateral branches from the substance P immunoreactive sensory fibres which traverse the ganglia, and that these can subserve a short-loop reflex control over the excitability of the ganglionic neurons in advance of, and, or, in support of, or even independently of, the recruitment of central nervous circuits.

scholarly journals 425 Mast cells survival and maturation in human skin are regulated and maintained by sensory nerve fibers

2016 ◽  
Vol 136 (5) ◽  
pp. S75 ◽  
Author(s):  
J. Chéret ◽  
L. Ponce ◽  
C. Le Gall-Ianotto ◽  
L. Misery ◽  
M. Bertolini ◽  
...  
Keyword(s):  
Mast Cells ◽  
Human Skin ◽  
Sensory Nerve ◽  
Nerve Fibers
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