scholarly journals Detection of Equid herpesvirus‐1 in serum samples collected from infected horses

2021 ◽  
Vol 53 (S56) ◽  
pp. 55-55
Revista CERES ◽  
2014 ◽  
Vol 61 (2) ◽  
pp. 280-283 ◽  
Author(s):  
Marcus Rebouças Santos ◽  
Hanna Carolina Campos Ferreira ◽  
Marcos Antônio dos Santos ◽  
Giuliana Loreto Saraiva ◽  
Natália Filardi Tafuri ◽  
...  

Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In the past decades, the number of cases in Brazil has been gradually increasing. Therefore, it is important to assess the distribution of infection in different regions of the country. In the state of Espírito Santo (ES) the BoHV 1 infection rate in dairy cattle herds is unknown. Thus, the aim of this study was to detect neutralizing antibodies against BoHV-1 in serum samples from 1,161 non-vaccinated cows from 59 dairy cattle herds in 23 municipalities of the Metropolitan, North, Northwest and South macro-regions. The identification of seropositive cows was evaluated by the virus neutralization test. The results showed that of all serum samples evaluated 775 (66.75%) had neutralizing antibodies against BoHV-1. Moreover, all herds were found positive; however, the percentage of positive cows varied among regions; 49.06%, 62.15%, 67.21% and 80.04% for the Metropolitan, South, North and Northwest macro-regions, respectively. In this study, the results clearly indicate the dissemination of the viral agent in dairy cattle in the ES state, requiring the monitoring and control of diseases related to BoHV-1 infection.


1991 ◽  
Vol 53 (1) ◽  
pp. 133-135
Author(s):  
Nobuo KOIZUMI ◽  
Eiichi HONDA ◽  
Tetsuo KUMAGAI ◽  
Katsunori OKAZAKI

1993 ◽  
Vol 55 (1) ◽  
pp. 119-121 ◽  
Author(s):  
Mamiko INAZU ◽  
Osamu TSUHA ◽  
Rikio KIRISAWA ◽  
Yoshimi KAWAKAMI ◽  
Hiroshi IWAI

1996 ◽  
Vol 115 (4) ◽  
pp. 385-397 ◽  
Author(s):  
J.P. Tearle ◽  
K.C. Smith ◽  
M.S. Boyle ◽  
M.M. Binns ◽  
G.J. Livesay ◽  
...  

1989 ◽  
Vol 1 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Fernando Osorio ◽  
Subramaniam Srikumaran ◽  
Marvin Rhodes ◽  
David Christensen ◽  
Pushpa Srikumaran

The detection of virus-specific immunoglobulin M (IgM) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. We have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine IgM to separate the whole IgM fraction of a bovine serum sample. The IgM specific for bovine herpesvirus-1 (BHV-1) is then detected by the addition of viral antigen, which in turn is detected by BHV-1-specific monoclonal antibodies conjugated to horseradish peroxidase. A BHV-1 IgM antibody response was detected during the early postinfection period (7–40 days PI). Bovine herpesvirus-1 IgM antibody was not detected in sera taken from 3 animals following dexamethasone-induced viral reactivation. This method compares favorably with viral isolation, antigen detection in the clinical samples, and paired serology in the diagnosis of BHV-1 infection at a herd level.


1984 ◽  
Vol 114 (15) ◽  
pp. 369-374 ◽  
Author(s):  
R. Burrows ◽  
D. Goodridge ◽  
M. Denyer

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