scholarly journals Stepwise accumulation of mutations in CesA3 in Phytophthora sojae results in increasing resistance to CAA fungicides

2020 ◽  
Author(s):  
Meng Cai ◽  
Can Zhang ◽  
Weizhen Wang ◽  
Qin Peng ◽  
Xi Song ◽  
...  
Keyword(s):  
Phytophthora Sojae ◽  
Caa Fungicides

scholarly journals An FYVE-Domain-Containing Protein, PsFP1, Is Involved in Vegetative Growth, Oxidative Stress Response and Virulence of Phytophthora sojae

2021 ◽  
Vol 22 (12) ◽  
pp. 6601
Author(s):  
Jinhui Zhang ◽  
Xiaoran Du ◽  
Xin Zhou ◽  
Duo Jin ◽  
Jianqiang Miao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Membrane Trafficking ◽  
Vegetative Growth ◽  
Genome Mining ◽  
Gene Replacement ◽  
Wide Distribution ◽  
Stress Sensitivity ◽  
Phytophthora Sojae ◽  
Knock Out ◽  
Fyve Domain

Proteins that contain the FYVE zinc-finger domain are recruited to PtdIns3P-containing membranes, participating in numerous biological processes such as membrane trafficking, cytoskeletal regulation, and receptor signaling. However, the genome-wide distribution, evolution, and biological functions of FYVE-containing proteins are rarely reported for oomycetes. By genome mining of Phytophthora sojae, two proteins (PsFP1 and PsFP2) with a combination of the FYVE domain and the PX domain (a major phosphoinositide binding module) were found. To clarify the functions of PsFP1 and PsFP2, the CRISPR/Cas9-mediated gene replacement system was used to knock out the two genes respectively. Only heterozygous deletion mutants of PsFP1 were recovered, and the expression level of PsFP1 in the heterozygous knockout transformants was significantly down-regulated. These PsFP1 mutants showed a decrease in mycelial growth and pathogenicity and were more sensitive to hydrogen peroxide. These phenotypes were recovered to the level of wild-type by overexpression PsFP1 gene in the PsFP1 heterozygous knockout transformant. In contrast, deletion of PsFP2 had no significant effect on vegetative growth, asexual and sexual reproduction, pathogenicity, or oxidative stress sensitivity. PsFP1 was primarily localized in vesicle-like structures and both the FYVE and PX domains are important for its localization. Overall, our results indicate that PsFP1 plays an important role in the vegetative growth and virulence of P. sojae.

scholarly journals A CRISPR/Cas9‐mediated in situ complementation method for Phytophthora sojae mutants

2021 ◽  
Vol 22 (3) ◽  
pp. 373-381
Author(s):  
Min Qiu ◽  
Yaning Li ◽  
Wenwu Ye ◽  
Xiaobo Zheng ◽  
Yuanchao Wang
Keyword(s):  
Phytophthora Sojae

Identification of three closely linked loci conferring broad-spectrum Phytophthora sojae resistance in soybean variety Tosan-231

2021 ◽  
Author(s):  
Jun-ichi Matsuoka ◽  
Mami Takahashi ◽  
Tetsuya Yamada ◽  
Yuhi Kono ◽  
Naohiro Yamada ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Phytophthora Sojae ◽  
Soybean Variety

Analysis of pathogenicity and genetic variation among Phytophthora sojae isolates using RAPD

1999 ◽  
Vol 103 (2) ◽  
pp. 173-178 ◽  
Author(s):  
X.Q. Meng ◽  
R.C. Shoemaker ◽  
X.B. Yang
Keyword(s):  
Genetic Variation ◽  
Phytophthora Sojae

Borrelidin, a Potent Antifungal Agent: Insight into the Antifungal Mechanism against Phytophthora sojae

2012 ◽  
Vol 60 (39) ◽  
pp. 9874-9881 ◽  
Author(s):  
Ya-Mei Gao ◽  
Xiang-Jing Wang ◽  
Ji Zhang ◽  
Ming Li ◽  
Chong-Xi Liu ◽  
...  
Keyword(s):  
Antifungal Agent ◽  
Phytophthora Sojae ◽  
Antifungal Mechanism ◽  
Insight Into

scholarly journals Genetic Mapping and Molecular Characterization of a Broad-spectrum Phytophthora sojae Resistance Gene in Chinese Soybean

2019 ◽  
Vol 20 (8) ◽  
pp. 1809 ◽  
Author(s):  
Chao Zhong ◽  
Yinping Li ◽  
Suli Sun ◽  
Canxing Duan ◽  
Zhendong Zhu
Keyword(s):  
Genetic Mapping ◽  
Candidate Gene ◽  
Broad Spectrum ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Genetic Population ◽  
Phytophthora Root Rot ◽  
Different Origins ◽  
Lrr Domain

Phytophthora root rot (PRR) causes serious annual soybean yield losses worldwide. The most effective method to prevent PRR involves growing cultivars that possess genes conferring resistance to Phytophthora sojae (Rps). In this study, QTL-sequencing combined with genetic mapping was used to identify RpsX in soybean cultivar Xiu94-11 resistance to all P. sojae isolates tested, exhibiting broad-spectrum PRR resistance. Subsequent analysis revealed RpsX was located in the 242-kb genomic region spanning the RpsQ locus. However, a phylogenetic investigation indicated Xiu94-11 carrying RpsX is distantly related to the cultivars containing RpsQ, implying RpsX and RpsQ have different origins. An examination of candidate genes revealed RpsX and RpsQ share common nonsynonymous SNP and a 144-bp insertion in the Glyma.03g027200 sequence encoding a leucine-rich repeat (LRR) region. Glyma.03g027200 was considered to be the likely candidate gene of RpsQ and RpsX. Sequence analyses confirmed that the 144-bp insertion caused by an unequal exchange resulted in two additional LRR-encoding fragments in the candidate gene. A marker developed based on the 144-bp insertion was used to analyze the genetic population and germplasm, and proved to be useful for identifying the RpsX and RpsQ alleles. This study implies that the number of LRR units in the LRR domain may be important for PRR resistance in soybean.

scholarly journals Patterns of Gene Expression Upon Infection of Soybean Plants by Phytophthora sojae

2004 ◽  
Vol 17 (10) ◽  
pp. 1051-1062 ◽  
Author(s):  
Pat Moy ◽  
Dinah Qutob ◽  
B. Patrick Chapman ◽  
Ian Atkinson ◽  
Mark Gijzen
Keyword(s):  
Gene Expression ◽  
Time Course ◽  
Phytophthora Sojae ◽  
Gene Microarray ◽  
Pathogenesis Related ◽  
Soybean Plants ◽  
Related Proteins ◽  
Phytoalexin Biosynthesis ◽  
Host Tissues ◽  
Mixed Samples

To investigate patterns of gene expression in soybean (Glycine max) and Phytophthora sojae during an infection time course, we constructed a 4,896-gene microarray of host and pathogen cDNA transcripts. Analysis of rRNA from soybean and P. sojae was used to estimate the ratio of host and pathogen RNA present in mixed samples. Large changes in this ratio occurred between 12 and 24 h after infection, reflecting the rapid growth and proliferation of the pathogen within host tissues. From the microarray analysis, soybean genes that were identified as strongly upregulated during infection included those encoding enzymes of phytoalexin biosynthesis and defense and pathogenesis-related proteins. Expression of these genes generally peaked at 24 h after infection. Selected lipoxygenases and peroxidases were among the most strongly downregulated soybean genes during the course of infection. The number of pathogen genes expressed during infection reached a maximum at 24 h. The results show that it is possible to use a single microarray to simultaneously probe gene expression in two interacting organisms. The patterns of gene expression we observed in soybean and P. sojae support the hypothesis that the pathogen transits from biotrophy to necrotrophy between 12 and 24 h after infection.

scholarly journals Response of Soybean Pathogens to Glyceollin

2010 ◽  
Vol 100 (9) ◽  
pp. 897-903 ◽  
Author(s):  
Anatoly V. Lygin ◽  
Curtis B. Hill ◽  
Olga V. Zernova ◽  
Laura Crull ◽  
Jack M. Widholm ◽  
...  
Keyword(s):  
Hairy Root ◽  
Degradation Products ◽  
Macrophomina Phaseolina ◽  
Phytophthora Sojae ◽  
Plant Diseases ◽  
Phialophora Gregata ◽  
Diaporthe Phaseolorum ◽  
Significant Difference ◽  
Transformed Hairy Root ◽  
Soybean Genotypes

Plants recognize invading pathogens and respond biochemically to prevent invasion or inhibit colonization in plant cells. Enhancing this response in crop plants could improve sustainable methods to manage plant diseases. To enhance disease resistance in soybean, the soybean phytoalexin glyceollin was assessed in soybean hairy roots of two soybean genotypes, Spencer and PI 567374, transformed with either soybean isoflavone synthase (IFS2) or chalcone synthase (CHS6) genes that were inoculated with the soybean pathogens Diaporthe phaseolorum var. meridionales, Macrophomina phaseolina, Sclerotinia sclerotiorum, and Phytophthora sojae. The hairy-root-transformed lines had several-fold decreased levels of isoflavone daidzein, the precursor of glyceollin, and considerably lower concentrations of glyceollin induced by pathogens measured 5 days after fungal inoculation compared with the nontransformed controls without phenolic transgenes. M. phaseolina, P. sojae, and S. sclerotiorum grew much more on IFS2- and CHS6-transformed roots than on control roots, although there was no significant difference in growth of D. phaseolorum var. meridionales on the transformed hairy-root lines. In addition, glyceollin concentration was lower in D. phaseolorum var. meridionales-inoculated transformed and control roots than roots inoculated with the other pathogens. Glyceollin inhibited the growth of D. phaseolorum var. meridionales, M. phaseolina, P. sojae, S. sclerotiorum, and three additional soybean pathogens: Cercospora sojina, Phialophora gregata, and Rhizoctonia solani. The most common product of glyceollin conversion or degradation by the pathogens, with the exception of P. sojae, which had no glyceollin degradation products found in the culture medium, was 7-hydroxyglyceollin.

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