scholarly journals Genetic Mapping and Molecular Characterization of a Broad-spectrum Phytophthora sojae Resistance Gene in Chinese Soybean

2019 ◽  
Vol 20 (8) ◽  
pp. 1809 ◽  
Author(s):  
Chao Zhong ◽  
Yinping Li ◽  
Suli Sun ◽  
Canxing Duan ◽  
Zhendong Zhu
Keyword(s):  
Genetic Mapping ◽  
Candidate Gene ◽  
Broad Spectrum ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Genetic Population ◽  
Phytophthora Root Rot ◽  
Different Origins ◽  
Lrr Domain

Phytophthora root rot (PRR) causes serious annual soybean yield losses worldwide. The most effective method to prevent PRR involves growing cultivars that possess genes conferring resistance to Phytophthora sojae (Rps). In this study, QTL-sequencing combined with genetic mapping was used to identify RpsX in soybean cultivar Xiu94-11 resistance to all P. sojae isolates tested, exhibiting broad-spectrum PRR resistance. Subsequent analysis revealed RpsX was located in the 242-kb genomic region spanning the RpsQ locus. However, a phylogenetic investigation indicated Xiu94-11 carrying RpsX is distantly related to the cultivars containing RpsQ, implying RpsX and RpsQ have different origins. An examination of candidate genes revealed RpsX and RpsQ share common nonsynonymous SNP and a 144-bp insertion in the Glyma.03g027200 sequence encoding a leucine-rich repeat (LRR) region. Glyma.03g027200 was considered to be the likely candidate gene of RpsQ and RpsX. Sequence analyses confirmed that the 144-bp insertion caused by an unequal exchange resulted in two additional LRR-encoding fragments in the candidate gene. A marker developed based on the 144-bp insertion was used to analyze the genetic population and germplasm, and proved to be useful for identifying the RpsX and RpsQ alleles. This study implies that the number of LRR units in the LRR domain may be important for PRR resistance in soybean.

scholarly journals A giant chimeric NLR gene confers extreme broad-spectrum resistance to a plant pathogen

2021 ◽  
Author(s):  
Jianxin Ma ◽  
Weidong Wang ◽  
Liyang Chen ◽  
Kevin Fengler ◽  
Joy Bolar ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Gene Copy Number ◽  
Gene Clusters ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Gene Copy ◽  
Gene Copy Number Variation ◽  
Promoter Fusion ◽  
Broad Spectrum Resistance ◽  
Number Variation

Abstract Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae is the most destructive soybean soil-borne disease worldwide. Discovery of genes conferring broad-spectrum resistance to the pathogen is an urgent need to prevent the outbreak of the disease. Here we show that soybean Rps11 is a 27.7-kb nucleotide-binding site-leucine-rich repeat (NBS-LRR or NLR) gene conferring extreme broad-spectrum resistance to the pathogen. Rps11 is located in a genomic region harboring a cluster of unusually large NLR genes belonging to a single evolutionary lineage that is distinct from all other lineages in the soybean genome, and was derived from rounds of intergenic and intragenic unequal recombination. Such recombination events have resulted in promoter fusion and expansion of the LRR domain that presumably explains such broadness of the resistance spectrum. The NLR gene cluster exhibits drastic structural diversification among phylogenetically representative varieties, including gene copy number variation ranging from five to 23 copies, and absence of allelic copies of Rps11 (i.e., rps11) in any of the non-Rps11-donor varieties examined. Our study thus exemplifies innovative evolution of NLR genes and NLR gene clusters and will accelerate the deployment of Rps11 for soybean protection.

scholarly journals Fine mapping of a Phytophthora -resistance locus RpsGZ in soybean using genotyping-by-sequencing

2020 ◽  
Author(s):  
Bingzhi Jiang ◽  
Yanbo Cheng ◽  
Zhandong Cai ◽  
Mu Li ◽  
Ze Jiang ◽  
...  
Keyword(s):  
Fine Mapping ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Genotyping By Sequencing ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Resistance Locus ◽  
Phytophthora Root Rot ◽  
Phytophthora Resistance

Abstract Background: Phytophthora root rot (PRR) caused by Phytophthora sojae ( P. sojae ), is one of the most serious limitation to soybean-production in the world. Identification of resistant gene(s) and incorporating them into elite varieties are an effective way for breeding to prevent soybean from being harmed by this disease. A valuable mapping population of 228 F 8:11 recombinant inbred lines (RILs) derived from a cross of resistant cultivar Guizao1 and susceptible cultivar BRSMG68 and a high-density genetic linkage map with an average distance of 0.81 centimorgan (cM) between adjacent bin markers in this population were used to map and explore the candidate gene(s).Results: In this study, the PRR resistance in Guizao1 was controlled by a single Mendelian locus, and was fine mapped to a 367.371-kb genomic region on chromosome 3 that harbours 19 genes, including 7 disease resistance (R)-like genes in the reference Willliams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed that Glyma.03g05300 was likely involved in PRR resistance.Conclusions: These findings of fine mapping of a novel Rps locus will serve as a basis for cloning, transferring of resistant genes and breeding of P. sojae resistant soybean cultivars through marker-assisted selection.

scholarly journals A giant NLR gene confers broad-spectrum resistance to Phytophthora sojae in soybean

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weidong Wang ◽  
Liyang Chen ◽  
Kevin Fengler ◽  
Joy Bolar ◽  
Victor Llaca ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Gene Copy Number ◽  
Gene Clusters ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Gene Copy ◽  
Gene Copy Number Variation ◽  
Promoter Fusion ◽  
Broad Spectrum Resistance ◽  
Number Variation

AbstractPhytophthora root and stem rot caused by P. sojae is a destructive soybean soil-borne disease found worldwide. Discovery of genes conferring broad-spectrum resistance to the pathogen is a need to prevent the outbreak of the disease. Here, we show that soybean Rps11 is a 27.7-kb nucleotide-binding site-leucine-rich repeat (NBS-LRR or NLR) gene conferring broad-spectrum resistance to the pathogen. Rps11 is located in a genomic region harboring a cluster of large NLR genes of a single origin in soybean, and is derived from rounds of unequal recombination. Such events result in promoter fusion and LRR expansion that may contribute to the broad resistance spectrum. The NLR gene cluster exhibits drastic structural diversification among phylogenetically representative varieties, including gene copy number variation ranging from five to 23 copies, and absence of allelic copies of Rps11 in any of the non-Rps11-donor varieties examined, exemplifying innovative evolution of NLR genes and NLR gene clusters.

Evaluation of Molecular Markers Specific for 1BL∙1RS Translocation and Characterization of 1RS Chromosome in Wheat Varieties from Different Origins

2009 ◽  
Vol 35 (11) ◽  
pp. 2107-2115 ◽  
Author(s):  
Huai-Jun TANG ◽  
Gui-Hong YIN ◽  
Xian-Chun XIA ◽  
Jian-Jun FENG ◽  
Yan-Ying QU ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Wheat Varieties ◽  
Different Origins

Facile syringe filter-enabled bacteria separation, enrichment, and buffer exchange for clinical isolation-free digital detection and characterization of bacterial pathogens in urine

The Analyst ◽  
2021 ◽  
Author(s):  
Pengfei Zhang ◽  
Aniruddha Kaushik ◽  
Kathleen E Mach ◽  
Kuangwen Hsieh ◽  
Joseph C. Liao ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Antibiotic Therapy ◽  
Broad Spectrum ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Evidence Based ◽  
Pathogen Identification ◽  
Accelerated Methods ◽  
Syringe Filter

The development of accelerated methods for pathogen identification (ID) and antimicrobial susceptibility testing (AST) for infectious diseases is necessary to facilitate evidence-based antibiotic therapy and reduce clinical overreliance on broad-spectrum...

Identification of three closely linked loci conferring broad-spectrum Phytophthora sojae resistance in soybean variety Tosan-231

2021 ◽  
Author(s):  
Jun-ichi Matsuoka ◽  
Mami Takahashi ◽  
Tetsuya Yamada ◽  
Yuhi Kono ◽  
Naohiro Yamada ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Phytophthora Sojae ◽  
Soybean Variety

scholarly journals Streptogramin B biosynthesis in Streptomyces pristinaespiralis and Streptomyces virginiae: molecular characterization of the last structural peptide synthetase gene.

1997 ◽  
Vol 41 (9) ◽  
pp. 1904-1909 ◽  
Author(s):  
V de Crécy-Lagard ◽  
W Saurin ◽  
D Thibaut ◽  
P Gil ◽  
L Naudin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Activation ◽  
Activation Domains ◽  
Amino Acid Activation ◽  
Streptomyces Virginiae ◽  
Streptomyces Pristinaespiralis ◽  
Degenerate Oligonucleotide ◽  
Peptide Synthetase ◽  
Different Origins

Streptomyces pristinaespiralis and S. virginiae both produce closely related hexadepsipeptide antibiotics of the streptogramin B family. Pristinamycins I and virginiamycins S differ only in the fifth incorporated precursor, di(mono)methylated amine and phenylalanine, respectively. By using degenerate oligonucleotide probes derived from internal sequences of the purified S. pristinaespiralis SnbD and SnbE proteins, the genes from two streptogramin B producers, S. pristinaespiralis and S. virginiae, encoding the peptide synthetase involved in the activation and incorporation of the last four precursors (proline, 4-dimethylparaaminophenylalanine [for pristinamycin I(A)] or phenylalanine [for virginiamycin S], pipecolic acid, and phenylglycine) were cloned. Analysis of the sequence revealed that SnbD and SnbE are encoded by a unique snbDE gene. SnbDE (4,849 amino acids [aa]) contains four amino acid activation domains, four condensation domains, an N-methylation domain, and a C-terminal thioesterase domain. Comparison of the sequences of 55 amino acid-activating modules from different origins confirmed that these sequences contain enough information for the performance of legitimate predictions of their substrate specificity. Partial sequencing (1,993 aa) of the SnbDE protein of S. virginiae allowed comparison of the proline and aromatic acid activation domains of the two species and the identification of coupled frameshift mutations.

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