Granulocyte-macrophage colony-stimulating factor improves mouse peripheral nerve regeneration following sciatic nerve crush

2018 ◽  
Vol 48 (5) ◽  
pp. 2152-2164 ◽  
Author(s):  
André Luis Bombeiro ◽  
Bruna Toledo Nunes Pereira ◽  
Alexandre Leite Rodrigues de Oliveira
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Crush ◽  
Colony Stimulating Factor ◽  
Nerve Crush ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

scholarly journals Photobiomodulation in Sciatic Nerve Crush Injuries in Rodents: A Systematic Review of the Literature and Perspectives for Clinical Treatment

2020 ◽  
Vol 11 (3) ◽  
pp. 332-344
Author(s):  
Letícia Lemes Sasso ◽  
Luana Gabriel de Souza ◽  
Carlos Eduardo Girasol ◽  
Alexandre Márcio Marcolino ◽  
Rinaldo Roberto de Jesus Guirro ◽  
...  
Keyword(s):  
Energy Density ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Experimental Studies ◽  
Therapeutic Window ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Positive Effects

Objective: The aim of the study was to perform a literature review to analyze the effect of photobiomodulation in experimental studies on peripheral nerve regeneration after sciatic nerve crush injury in rodents. Methods: A bibliographic search was performed in the electronic databases, including MEDLINE (PubMed), SCOPUS, and SciELO, from 2008 to 2018. Results: A total of 1912 articles were identified in the search, and only 19 fulfilled all the inclusion criteria. Along with the parameters most found in the manuscripts, the most used wavelengths were 660 nm and 830 nm, power of 30 and 40 mW, and energy density of 4 and 10 J/cm2 . For total energy throughout the intervention period, the lowest energy found with positive effects was 0.70 J, and the highest 1.141 J. Seventeen studies reported positive effects on nerve regeneration. The variables selected to analyze the effect were: Sciatic Functional Index (SFI), Static Sciatic Index (SSI), morphometric, morphological, histological, zymographic, electrophysiological, resistance mechanics and range of motion (ROM). The variety of parameters used in the studies demonstrated that there is yet no pre-determined protocol for treating peripheral nerve regeneration. Only two studies by different authors used the same power, energy density, beam area, and power density. Conclusion: It was concluded that the therapeutic window of the photobiomodulation presents a high variability of parameters with the wavelength (632.8 to 940 nm), power (5 to 170 mW) and energy density (3 to 280 J /cm2 ), promoting nerve regeneration through the expression of cytokines and growth factors that aid in modulating the inflammatory process, improving morphological aspects, restoring the functionality to the animals in a brief period.

scholarly journals An Index of Topographic Normality in Rat Somatosensory Cortex: Application to a Sciatic Nerve Crush Model

2002 ◽  
Vol 88 (3) ◽  
pp. 1339-1351 ◽  
Author(s):  
Scott Barbay ◽  
Eric K. Peden ◽  
Gerald Falchook ◽  
Randolph J. Nudo
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Somatosensory Cortex ◽  
Functional Recovery ◽  
Peripheral Nerve Regeneration ◽  
Control Group ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Somatotopic Organization ◽  
Cortical Topography

Previous studies have demonstrated that peripheral denervation of the skin is reflected in the CNS as a reorganization of somatotopic representations. In cases in which peripheral nerve regeneration occurs there is a gradual reactivation of cortex by novel receptive fields that is reversed as regenerated nerves reestablish connections with the original skin surface. Functional recovery appears to depend on the pattern in which somatotopic organization in the cortex is reestablished. The relationship between functional recovery and cortical topography is not precise, however, since the descriptions of postinjury representations in the cortex have been largely descriptive and not quantitative. The purpose of this study was to derive an index to quantify deviations from normal somatotopic organization in the somatosensory cortex. Multiunit recordings of cutaneous representations in the somatosensory cortex (S1) of the rat were defined using Semmes-Weinstein monofilaments to stimulate the skin over the distal hindlimb of the rat 2 and 4 months after a sciatic nerve crush. To derive a sensitive index of topography, the sciatic nerve crush was selected as the injury model since nerve regeneration following crush injuries has been reported to reinstate preinjury cortical topography. Group comparisons were made with an intact control group. The results show that there were subtle, but significant differences in topography between rats with a regenerated sciatic nerve and normal rats. In addition, average thresholds for evoking cortical responses were higher than normal (but within normal range) 2 and 4 months after the crush. These results demonstrate that the index of topography derived for this study can reveal deviations that may not be distinguishable from normal topography when based on qualitative descriptions.

Cinnamaldehyde Enhanced Functional Recovery after Sciatic Nerve Crush Injury in Rats

2020 ◽  
Vol 209 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Zohreh Jahromi ◽  
Fahimeh Mohammadghasemi ◽  
Farshad Moharrami Kasmaie ◽  
Arash Zaminy
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Crush Injury ◽  
Vehicle Group ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Clinical Issue ◽  
Study Results

Peripheral nerve injury is a common clinical issue induced by trauma, tumor, and damage caused by treatment. Such factors create chemical and inflammatory alterations at the injury site, which increase nerve deterioration. Thus, minimizing these modifications can lead to nerve protection after injury. The present study sought to evaluate the possible improvement in nerve regeneration and enhancement of functional outcomes by cinnamaldehyde (Cin) administration following sciatic nerve crush in a rat model. Rats (n = 48) were distributed into 6 groups, including sham, injury, DMSO (vehicle group), and Cin groups (10, 30, and 90 mg/kg/day). Using small hemostatic forceps, crush injury was induced in the left sciatic nerve. Thereafter, Cin was administered for 28 successive days. Weekly records were taken for sciatic functional index (SFI) measurements. Further assessments including electrophysiological and histomorphometric evaluations, gastrocnemius muscle wet weight measurements, and estimation of the serum total oxidant status were performed. According to the results, Cin could accelerate sciatic nerve recovery after crush injury, and the dose of 30 mg/kg/day of Cin had better impacts on SFI recovery, muscle mass ratio, and myelin content. The current research demonstrated that Cin positively affects peripheral nerve restoration. Therefore, Cin therapy could be considered as a potential treatment method for peripheral nerve regeneration and its functional recovery. However, more investigations are required to further validate the study results and evaluate the optimal dose of Cin.

scholarly journals STING regulates peripheral nerve regeneration and colony stimulating factor 1 receptor (CSF1R) processing in microglia

iScience ◽  
2021 ◽  
pp. 103434
Author(s):  
Giulio Morozzi ◽  
Julian Rothen ◽  
Gauthier Toussaint ◽  
Katrina De Lange ◽  
Katrin Westritschnig ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

scholarly journals Transcriptional Profiling at High Temporal Resolution Reveals Robust Immune/Inflammatory Responses during Rat Sciatic Nerve Recovery

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Lingyan Xing ◽  
Qiong Cheng ◽  
Guangbin Zha ◽  
Sheng Yi
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Temporal Resolution ◽  
Inflammatory Responses ◽  
Acute Injury ◽  
Nerve Degeneration ◽  
High Temporal Resolution ◽  
Sciatic Nerve Crush ◽  
Nerve Crush

After peripheral nerve injury, immune/inflammatory responses are triggered, which are critical for nerve regeneration. Despite their importance, the underlying molecular changes in immune/inflammatory responses remain largely unknown. In this study, we systematically analyzed differentially expressed genes in immune/inflammatory-related pathways at high temporal resolution and experimentally validated gene expression changes with RT-PCR following sciatic nerve crush in rats. We found that immune/inflammatory reactions not only occur in the acute injury but also remained activated over two weeks after injury. Detailed bioinformatic studies suggested that multiple immune/inflammatory pathways, including agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis, IL-6 signaling, and IL-10 signaling, were sustained activated during nerve degeneration and regeneration. Our current study expands our understanding of the molecular basis of altered immune/inflammatory-related pathways following injury and thus might offer the possibility of targeting related molecules as therapeutic intervention for peripheral nerve regeneration.

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