Open flow microperfusion: pharmacokinetics of human insulin and insulin detemir in the interstitial fluid of subcutaneous adipose tissue

2014 ◽  
Vol 17 (2) ◽  
pp. 121-127 ◽  
Author(s):  
C. Höfferer ◽  
D. Tutkur ◽  
C. Fledelius ◽  
C. L. Brand ◽  
T. J. Alsted ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Insulin ◽  
Subcutaneous Adipose Tissue ◽  
Insulin Detemir ◽  
Interstitial Fluid ◽  
Open Flow ◽  
Subcutaneous Adipose

Open-Flow Microperfusion of Subcutaneous Adipose Tissue for On-Line Continuous Ex Vivo Measurement of Glucose Concentration

Diabetes Care ◽  
1997 ◽  
Vol 20 (7) ◽  
pp. 1114-1121 ◽  
Author(s):  
Z. Trajanoski ◽  
G. A. Brunner ◽  
L. Schaupp ◽  
M. Ellmerer ◽  
P. Wach ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glucose Concentration ◽  
Subcutaneous Adipose Tissue ◽  
Ex Vivo ◽  
Open Flow ◽  
On Line ◽  
Subcutaneous Adipose

Quantification of Basal Insulin Peglispro and Human Insulin in Adipose Tissue Interstitial Fluid by Open-Flow Microperfusion

2017 ◽  
Vol 19 (5) ◽  
pp. 305-314 ◽  
Author(s):  
Katrin Tiffner ◽  
Beate Boulgaropoulos ◽  
Christian Höfferer ◽  
Thomas Birngruber ◽  
Niels Porksen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Insulin ◽  
Basal Insulin ◽  
Interstitial Fluid ◽  
Open Flow ◽  
Tissue Interstitial Fluid

Bioavailability of insulin detemir and human insulin at the level of peripheral interstitial fluid in humans, assessed by open-flow microperfusion

2015 ◽  
Vol 17 (12) ◽  
pp. 1166-1172 ◽  
Author(s):  
M. Bodenlenz ◽  
M. Ellmerer ◽  
L. Schaupp ◽  
L. V. Jacobsen ◽  
J. Plank ◽  
...  
Keyword(s):  
Human Insulin ◽  
Insulin Detemir ◽  
Interstitial Fluid ◽  
Open Flow

Subcutaneous adipose tissue exerts proinflammatory cytokines after minimal trauma in humans

2007 ◽  
Vol 293 (3) ◽  
pp. E690-E696 ◽  
Author(s):  
Christoph Pachler ◽  
Dimas Ikeoka ◽  
Johannes Plank ◽  
Heinz Weinhandl ◽  
Maria Suppan ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Fold Increase ◽  
Local Effect ◽  
Cytokine Response ◽  
Open Flow ◽  
Tnf Α ◽  
Minimal Trauma ◽  
Inflammatory Cytokine Response ◽  
Subcutaneous Adipose

Inflammatory cytokines released from adipose tissue play an important role in different pathological processes. In the present study, we investigated the inflammatory cytokine response of human subcutaneous adipose tissue (SAT) by applying the open-flow microperfusion technique. Four standard 18-gauge microperfusion catheters were inserted into periumbilical SAT of eight healthy male volunteers [29 ± 3 yr, BMI 24.3 ± 1.9 (mean ± SD)]. SAT probe effluents were collected at 60-min intervals for 8 h after catheter insertion. Different perfusion fluids were used to measure the local effect of insulin and/or glucose on the cytokine response. SAT probe effluents were analyzed for IL-1β, IL-6, CXCL8 (IL-8), and TNF-α. SAT concentrations of IL-1β increased 100-fold from 1.0 ± 0.2 pg/ml (mean ± SE) to 101.5 ± 23.2 pg/ml ( P < 0.001) after 8 h. A 130-fold increase was observed for CXCL8, from 49 ± 29 to 6,554 ± 1,713 pg/ml ( P < 0.001). Furthermore, a 20-fold increase of IL-6 was observed within the first 5 h (from 159 ± 123 to 3,554 ± 394 pg/ml; P < 0.001), and a significant decline to 2,154 ± 216 pg/ml ( P < 0.01) was seen thereafter. Finally, TNF-α increased from 1.4 ± 0.6 to 2.5 ± 0.5 pg/ml ( P < 0.05) in hour 2 and remained stable thereafter. Local administration of insulin exerted a stimulatory effect on the inflammatory response of IL-6. In conclusion, SAT exerts a highly reproducible and consistent proinflammatory cytokine response after minimally invasive trauma caused by the insertion of a catheter in humans.

Interstitial Glycerol Concentration in Human Skeletal Muscle and Adipose Tissue is Close to the Concentration in Blood

1996 ◽  
Vol 90 (6) ◽  
pp. 453-456 ◽  
Author(s):  
Jaswinder S. Samra ◽  
Cate L. Ravell ◽  
Sarah L. Giles ◽  
Peter Arner ◽  
Keith N. Frayn
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Interstitial Fluid ◽  
Normal Subjects ◽  
Glycerol Concentration ◽  
Free Glycerol ◽  
Arterial Concentration ◽  
Subcutaneous Adipose ◽  
Arteriovenous Difference

1. The suggestion that the interstitial glycerol concentration in both adipose tissue and skeletal muscle is around 3 mmol/l (Maggs DG, Jacob R, Rife F, et al. J Clin Invest 1995; 96: 370–7), rather than close to the blood concentration as previously supposed, was tested by independent methods. 2. Free glycerol was infused, as part of a triacylglycerol emulsion, into six normal subjects and the arteriovenous difference for glycerol across the forearm was measured. In addition the relative interstitial glycerol concentration in subcutaneous adipose tissue was assessed simultaneously in four of the subjects by microdialysis. 3. During glycerol infusion the arterialized glycerol concentration rose from 52 ± 5 μmol/l to 250–300 μmol/l (P < 0.001) in a square wave fashion. The net arteriovenous difference for glycerol across the forearm changed from negative (output) to positive (uptake) (P < 0.01). In subcutaneous adipose tissue the interstitial glycerol concentration rose during glycerol infusion (P < 0.001). 4. These observations are most easily explained by the movement of glycerol from plasma to interstitial fluid down a concentration gradient. We conclude that the interstitial glycerol concentration in skeletal muscle and adipose tissue is closer to the arterial concentration than to 3 mmol/l.

scholarly journals Lactate Metabolism of Subcutaneous Adipose Tissue Studied by Open Flow Microperfusion

1998 ◽  
Vol 83 (12) ◽  
pp. 4394-4401 ◽  
Author(s):  
M. Ellmerer ◽  
L. Schaupp ◽  
G. Sendlhofer ◽  
A. Wutte ◽  
G. A. Brunner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Lactate Metabolism ◽  
Open Flow ◽  
Subcutaneous Adipose

Absolute Lactate Concentration in Subcutaneous Adipose Tissue Measured by Open Flow Microperfusion

1998 ◽  
Vol 43 (s2) ◽  
pp. 155-157
Author(s):  
M. Ellmerer ◽  
L. Schaupp ◽  
G. Sendlhofer ◽  
A. Wutte ◽  
G.A. Brunner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Lactate Concentration ◽  
Open Flow ◽  
Subcutaneous Adipose
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