Quantification of Basal Insulin Peglispro and Human Insulin in Adipose Tissue Interstitial Fluid by Open-Flow Microperfusion

2017 ◽  
Vol 19 (5) ◽  
pp. 305-314 ◽  
Author(s):  
Katrin Tiffner ◽  
Beate Boulgaropoulos ◽  
Christian Höfferer ◽  
Thomas Birngruber ◽  
Niels Porksen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Insulin ◽  
Basal Insulin ◽  
Interstitial Fluid ◽  
Open Flow ◽  
Tissue Interstitial Fluid

Direct access to interstitial fluid in adipose tissue in humans by use of open-flow microperfusion

1999 ◽  
Vol 276 (2) ◽  
pp. E401-E408 ◽  
Author(s):  
L. Schaupp ◽  
M. Ellmerer ◽  
G. A. Brunner ◽  
A. Wutte ◽  
G. Sendlhofer ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glucose Concentration ◽  
Interstitial Fluid ◽  
Internal Standard ◽  
Ion Concentration ◽  
Direct Access ◽  
Open Flow ◽  
Hyperglycemic Clamp ◽  
Calibration Methods ◽  
Lumen Catheter

To gain direct access to the interstitial fluid (ISF), a new technique called open-flow microperfusion has been evaluated. This method is based on a double-lumen catheter with macroscopic (0.3–0.5 mm diameter) perforations that is inserted into the subcutaneous adipose tissue and constantly perfused. Thus partial equilibration between the ISF and the perfusion fluid occurs. The glucose concentration of the ISF was determined by established (zero flow rate, no net flux, and recirculation procedures) and new (ionic reference and suction technique) calibration methods by use of open-flow microperfusion. The data show that 1) the glucose concentration in the ISF is significantly lower than the corresponding arterialized venous plasma values during basal steady-state conditions (adipose tissue 3.2 ± 0.10 mM, plasma 5.27 ± 0.12 mM) as well as during hyperglycemic clamp experiments (adipose tissue 7.3 ± 0.13 mM, plasma 9.91 ± 0.16 mM), and 2) it is possible to determine the recovery continuously by using the ion concentration of the ISF as an internal standard (ionic reference).

Bioavailability of insulin detemir and human insulin at the level of peripheral interstitial fluid in humans, assessed by open-flow microperfusion

2015 ◽  
Vol 17 (12) ◽  
pp. 1166-1172 ◽  
Author(s):  
M. Bodenlenz ◽  
M. Ellmerer ◽  
L. Schaupp ◽  
L. V. Jacobsen ◽  
J. Plank ◽  
...  
Keyword(s):  
Human Insulin ◽  
Insulin Detemir ◽  
Interstitial Fluid ◽  
Open Flow

Measurement of interstitial albumin in human skeletal muscle and adipose tissue by open-flow microperfusion

2000 ◽  
Vol 278 (2) ◽  
pp. E352-E356 ◽  
Author(s):  
Martin Ellmerer ◽  
Lukas Schaupp ◽  
Gernot A. Brunner ◽  
Gerald Sendlhofer ◽  
Andrea Wutte ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Interstitial Fluid ◽  
Linear Regression Analysis ◽  
Albumin Concentration ◽  
Calibration Technique ◽  
Open Flow ◽  
Healthy Humans ◽  
Net Flux ◽  
Flux Calibration

The absolute concentration of albumin was measured in the interstitial fluid of subcutaneous adipose tissue and skeletal muscle in six healthy volunteers by combining the method of open-flow microperfusion and the no-net-flux calibration technique. By use of open-flow microperfusion, four macroscopically perforated double lumen catheters were inserted into the tissue regions of interest and constantly perfused. Across the macroscopic perforations of the catheters interstitial fluid was partially recovered in the perfusion fluid. Catheters were perfused with five solutions, each containing different concentrations of albumin. Absolute interstitial albumin concentrations were calculated by applying linear regression analysis to perfusate vs. sampled albumin concentration (no-net-flux calibration technique). Interstitial albumin concentrations were significantly lower ( P < 0.0001) in adipose tissue (7.36 g/l; r = 0.99, P < 0.0003; range: 4.3–10.7 g/l) and in skeletal muscle (13.25 g/l; r = 0.99, P < 0.0012; range: 9.7 to 15.7 g/l) compared with the serum concentration (48.9 ± 0.7 g/l, mean ± SE, n = 6; range: 46.4–50.4 g/l). Furthermore, interstitial albumin concentrations were significantly higher in skeletal muscle compared with adipose tissue ( P < 0.01). The study indicates that open-flow microperfusion allows stable sampling of macromolecules from the interstitial space of peripheral tissue compartments. Moreover, the present data report for the first time in healthy humans in vivo the true albumin concentrations of interstitial fluid of adipose tissue and skeletal muscle.

Open-Flow Microperfusion of Subcutaneous Adipose Tissue for On-Line Continuous Ex Vivo Measurement of Glucose Concentration

Diabetes Care ◽  
1997 ◽  
Vol 20 (7) ◽  
pp. 1114-1121 ◽  
Author(s):  
Z. Trajanoski ◽  
G. A. Brunner ◽  
L. Schaupp ◽  
M. Ellmerer ◽  
P. Wach ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glucose Concentration ◽  
Subcutaneous Adipose Tissue ◽  
Ex Vivo ◽  
Open Flow ◽  
On Line ◽  
Subcutaneous Adipose

Insulin and metabolic efficiency in rats. I. Effects of sucrose feeding and BAT axotomy

1986 ◽  
Vol 251 (6) ◽  
pp. R1109-R1117
Author(s):  
T. J. Bartness ◽  
C. J. Billington ◽  
A. S. Levine ◽  
J. E. Morley ◽  
D. M. Brown ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Basal Insulin ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Metabolic Efficiency ◽  
Insulin Deficiency ◽  
Lipoprotein Lipase Activity ◽  
Brown Adipose ◽  
Sucrose Feeding

The role of insulin and brown adipose tissue (BAT) thermogenesis in metabolic efficiency (ME, the efficiency of body wt gain) was examined in rats with varied basal insulin status. Long-lasting insulin was administered using a protocol that did not alter food intake, yet increased ME in both groups. Half the rats were fed sucrose to stimulate BAT growth and thermogenesis. Insulin overrode the exaggerated decrease in ME in sucrose-fed diabetics, with only partial attenuation in controls. Interscapular BAT (IBAT) lipoprotein lipase activity was decreased in diabetic rats, restored by insulin treatment, and not affected in controls. Sucrose-fed diabetics and controls had their IBAT sham or bilaterally surgically denervated. Insulin decreased the thermogenic potential of BAT [cytochrome oxidase activity (COA)] in intact controls and diabetics; in the latter, insulin restored COA independent of BAT innervation. We conclude that insulin can increase ME without an associated increase in energy intake, regardless of basal insulin status, both insulin deficiency and excess decrease BAT thermogenic potential (COA), and hyperinsulinemia-induced increases in ME may result from decreased BAT mitochondrial proliferation.

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