Draxin inhibits chick trunk neural crest delamination and migration by increasing cell adhesion

2021 ◽  
Author(s):  
Juan Du ◽  
Sanbing Zhang ◽  
Jiqian Zhao ◽  
Sha Li ◽  
Wenyong Chen ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Neural Crest ◽  
Trunk Neural Crest ◽  
And Migration

scholarly journals Neural crest cell migration: requirements for exogenous fibronectin and high cell density.

1983 ◽  
Vol 96 (2) ◽  
pp. 462-473 ◽  
Author(s):  
R A Rovasio ◽  
A Delouvee ◽  
K M Yamada ◽  
R Timpl ◽  
J P Thiery
Keyword(s):  
Cell Adhesion ◽  
Neural Crest ◽  
Type I Collagen ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Type I ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Crest Cell

Cells of the neural crest participate in a major class of cell migratory events during embryonic development. From indirect evidence, it has been suggested that fibronectin (FN) might be involved in these events. We have directly tested the role of FN in neural crest cell adhesion and migration using several in vitro model systems. Avian trunk neural crest cells adhered readily to purified plasma FN substrates and to extracellular matrices containing cellular FN. Their adhesion was inhibited by antibodies to a cell-binding fragment of FN. In contrast, these cells did not adhere to glass, type I collagen, or to bovine serum albumin in the absence of FN. Neural crest cell adhesion to laminin (LN) was significantly less than to FN; however, culturing of crest cells under conditions producing an epithelioid phenotype resulted in cells that could bind equally as well to LN as to FN. The migration of neural crest cells appeared to depend on both the substrate and the extent of cell interactions. Cells migrated substantially more rapidly on FN than on LN or type I collagen substrates; if provided a choice between stripes of FN and glass or LN, cells migrated preferentially on the FN. Migration was inhibited by antibodies against the cell-binding region of FN, and the inhibition could be reversed by a subsequent addition of exogenous FN. However, the migration on FN was random and displayed little persistence of direction unless cells were at high densities that permitted frequent contacts. The in vitro rate of migration of cells on FN-containing matrices was 50 microns/h, similar to their migration rates along the narrow regions of FN-containing extracellular matrix in migratory pathways in vivo. These results indicate that FN is important for neural crest cell adhesion and migration and that the high cell densities of neural crest cells in the transient, narrow migratory pathways found in the embryo are necessary for effective directional migration.

Role of collagen and fibronectin in neural crest cell adhesion and migration

1981 ◽  
Vol 87 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Judith H. Greenberg ◽  
Silja Seppä ◽  
Heikki Seppä ◽  
A.Tyl Hewitt
Keyword(s):  
Cell Adhesion ◽  
Neural Crest ◽  
Neural Crest Cell ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Crest Cell

Adhesion and migration of avian neural crest cells on fibronectin require the cooperating activities of multiple integrins of the (beta)1 and (beta)3 families

1999 ◽  
Vol 112 (24) ◽  
pp. 4715-4728 ◽  
Author(s):  
S. Testaz ◽  
M. Delannet ◽  
J. Duband
Keyword(s):  
Cell Adhesion ◽  
Neural Crest ◽  
Cell Population ◽  
Neural Crest Cells ◽  
Cellular Level ◽  
Extracellular Matrix Molecule ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Alpha V Beta 3

Based on genetic, functional and histological studies, the extracellular matrix molecule fibronectin has been proposed to play a key role in the migration of neural crest cells in the vertebrate embryo. In the present study, we have analyzed in vitro the repertoire and function of integrin receptors involved in the adhesive and locomotory responses of avian truncal neural crest cells to fibronectin. Immunoprecipitation experiments showed that neural crest cells express multiple integrins, namely (alpha)3(beta)1, (alpha)4(beta)1, (alpha)5(beta)1, (alpha)8(beta)1, (alpha)v(beta)1, (alpha)v(beta)3 and a (beta)8 integrin, as potential fibronectin receptors, and flow cytometry analyses revealed no major heterogeneity among the cell population for expression of integrin subunits. In addition, the integrin repertoire expressed by neural crest cells was found not to change dramatically during migration. At the cellular level, only (alpha)v(beta)1 and (alpha)v(beta)3 were concentrated in focal adhesion sites in connection with the actin microfilaments, whereas the other integrins were predominantly diffuse over the cell surface. In inhibition assays with function-perturbing antibodies, it appeared that complete abolition of cell spreading and migration could be achieved only by blocking multiple integrins of the (beta)1 and (beta)3 families, suggesting possible functional compensations between different integrins. In addition, these studies provided evidence for functional partitioning of integrins in cell adhesion and migration. While spreading was essentially mediated by (alpha)v(beta)1 and (alpha)8(beta)1, migration involved primarily (alpha)4(beta)1, (alpha)v(beta)3 and (alpha)8(beta)1 and, more indirectly, (alpha)3(beta)1. (alpha)5(beta)1 and the (beta)8 integrin were not found to play any major role in either adhesion or migration. Finally, consistent with the results of inhibition experiments, recruitment of (alpha)4(beta)1 and (alpha)v(beta)3, individually or in combination using antibodies or recombinant VCAM-1 and PECAM-1 molecules as a substratum, was required for migration but was not sufficient to produce migration of the cell population as efficiently as with fibronectin. In conclusion, our study indicates that neural crest cells express a multiplicity of fibronectin-binding integrins and suggests that dispersion of the cell population requires cooperation between distinct integrins regulating different events of cell adhesion, locomotion and, possibly, proliferation and survival.

scholarly journals Asymmetric localization of Dlc1 defines trunk neural crest polarity for directional delamination and migration

2017 ◽  
Vol 145 ◽  
pp. S65
Author(s):  
Martin Cheung ◽  
Jessica Aijia Liu ◽  
Yanxia Rao ◽  
May Pui Lai Cheung ◽  
Man-Ning Hui ◽  
...  
Keyword(s):  
Neural Crest ◽  
Trunk Neural Crest ◽  
And Migration

scholarly journals Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jessica Aijia Liu ◽  
Yanxia Rao ◽  
May Pui Lai Cheung ◽  
Man-Ning Hui ◽  
Ming-Hoi Wu ◽  
...  
Keyword(s):  
Neural Crest ◽  
Trunk Neural Crest ◽  
And Migration

Specific roles of the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins in avian neural crest cell adhesion and migration on vitronectin

Development ◽  
1994 ◽  
Vol 120 (9) ◽  
pp. 2687-2702 ◽  
Author(s):  
M. Delannet ◽  
F. Martin ◽  
B. Bossy ◽  
D.A. Cheresh ◽  
L.F. Reichardt ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Adhesion ◽  
Neural Crest ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Alpha V Beta 3 ◽  
Crest Cell

To identify potentially important extracellular matrix adhesive molecules in neural crest cell migration, the possible role of vitronectin and its corresponding integrin receptors was examined in the adhesion and migration of avian neural crest cells in vitro. Adhesion and migration on vitronectin were comparable to those found on fibronectin and could be almost entirely abolished by antibodies against vitronectin and by RGD peptides. Immunoprecipitation and immunocytochemistry analyses revealed that neural crest cells expressed primarily the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins as possible vitronectin receptors. Inhibition assays of cellular adhesion and migration with function-perturbing antibodies demonstrated that adhesion of neural crest cells to vitronectin was mediated essentially by one or more of the different alpha V integrins, with a possible preeminence of alpha V beta 1, whereas cell migration involved mostly the alpha V beta 3 and alpha V beta 5 integrins. Immunofluorescence labeling of cultured motile neural crest cells revealed that the alpha V integrins are differentially distributed on the cell surface. The beta 1 and alpha V subunits were both diffuse on the surface of cells and in focal adhesion sites in association with vinculin, talin and alpha-actinin, whereas the alpha V beta 3 and alpha V beta 5 integrins were essentially diffuse on the cell surface. Finally, vitronectin could be detected by immunoblotting and immunohistochemistry in the early embryo during the ontogeny of the neural crest. It was in particular closely associated with the surface of migrating neural crest cells. In conclusion, our study indicates that neural crest cells can adhere to and migrate on vitronectin in vitro by an RGD-dependent mechanism involving at least the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins and that these integrins may have specific roles in the control of cell adhesion and migration.

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