scholarly journals MicroRNA-29b regulates DNA methylation by targeting Dnmt3a/3b and Tet1/2/3 in porcine early embryo development

2018 ◽  
Vol 60 (4) ◽  
pp. 197-204 ◽  
Author(s):  
Zhiren Zhang ◽  
Yunqing Cao ◽  
Yanhui Zhai ◽  
Xiaoling Ma ◽  
Xinglan An ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Embryo Development ◽  
Early Embryo ◽  
Early Embryo Development

Absence of mitochondrial DNA methylation in mouse oocyte maturation, aging and early embryo development

2019 ◽  
Vol 513 (4) ◽  
pp. 912-918 ◽  
Author(s):  
Li-Hua Fan ◽  
Zhen-Bo Wang ◽  
Qian-Nan Li ◽  
Tie-Gang Meng ◽  
Ming-Zhe Dong ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Mouse Oocyte ◽  
Early Embryo Development

scholarly journals DNA methylation and functional characterization of the XIST gene during in vitro early embryo development in cattle

Epigenetics ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 568-588 ◽  
Author(s):  
Anelise Dos Santos Mendonça ◽  
Márcia Marques Silveira ◽  
Álvaro Fabrício Lopes Rios ◽  
Paula Magnelli Mangiavacchi ◽  
Alexandre Rodrigues Caetano ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Embryo Development ◽  
Functional Characterization ◽  
Early Embryo ◽  
Xist Gene ◽  
Early Embryo Development

scholarly journals Methylation dynamics during folliculogenesis and early embryo development in sheep

Reproduction ◽  
2017 ◽  
Vol 153 (5) ◽  
pp. 605-619 ◽  
Author(s):  
Laura Masala ◽  
Giovanni Pietro Burrai ◽  
Emanuela Bellu ◽  
Federica Ariu ◽  
Luisa Bogliolo ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Embryo Development ◽  
Environmental Changes ◽  
Temporal Analysis ◽  
Dna Methyltransferases ◽  
Early Embryo ◽  
Developmental Competence ◽  
Oocyte Growth ◽  
Differential Model ◽  
Early Embryo Development

Genome-wide DNA methylation reprogramming occurs during mammalian gametogenesis and early embryogenesis. Post-fertilization demethylation of paternal and maternal genomes is considered to occur by an active and passive mechanism respectively, in most mammals but sheep; in this species no loss of methylation was observed in either pronucleus. Post-fertilization reprogramming relies on methylating and demethylating enzymes and co-factors that are stored during oocyte growth, concurrently with the re-methylation of the oocyte itself. The crucial remodelling of the oocyte epigenetic baggage often overlaps with potential interfering events such as exposure to assisted reproduction technologies or environmental changes. Here, we report a temporal analysis of methylation dynamics during folliculogenesis and early embryo development in sheep. We characterized global DNA methylation and hydroxymethylation by immunofluorescence and relatively quantified the expression of the enzymes and co-factors mainly responsible for their remodelling (DNA methyltransferases (DNMTs), ten-eleven translocation (TET) proteins and methyl-CpG-binding domain (MBD) proteins). Our results illustrate for the first time the patterns of hydroxymethylation during oocyte growth. We observed different patterns of methylation and hydroxymethylation between the two parental pronuclei, suggesting that male pronucleus undergoes active demethylation also in sheep. Finally, we describe gene-specific accumulation dynamics for methylating and demethylating enzymes during oocyte growth and observe patterns of expression associated with developmental competence in a differential model of oocyte potential. Our work contributes to the understanding of the methylation dynamics during folliculogenesis and early embryo development and improves the overall picture of early rearrangements that will originate the embryo epigenome.

scholarly journals DNA methylation, environmental exposures and early embryo development

2019 ◽  
Vol 16 (3) ◽  
pp. 465-474
Author(s):  
Mélanie Breton-Larrivée ◽  
Elizabeth Elder ◽  
Serge McGraw
Keyword(s):  
Dna Methylation ◽  
Embryo Development ◽  
Environmental Exposures ◽  
Early Embryo ◽  
Early Embryo Development

Minor Splicing Factors Zrsr1 and Zrsr2 Essential for Gametogenesis, Early Embryo Development and Conversion of Stem Cells into 2C-Like

2019 ◽  
Author(s):  
Isabel Gómez-Redondo ◽  
Priscila Ramos-Ibeas ◽  
Eva Pericuesta ◽  
Benjamín Planells ◽  
Raul Fernández-González ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryo Development ◽  
Early Embryo ◽  
Splicing Factors ◽  
Early Embryo Development

scholarly journals LIN28 coordinately promotes nucleolar/ribosomal functions and represses the 2C-like transcriptional program in pluripotent stem cells

2021 ◽  
Author(s):  
Zhen Sun ◽  
Hua Yu ◽  
Jing Zhao ◽  
Tianyu Tan ◽  
Hongru Pan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryo Development ◽  
Pluripotent Stem Cells ◽  
Rna Binding ◽  
Stem Cell Differentiation ◽  
Early Embryo ◽  
Transcriptional Program ◽  
Cell Stage ◽  
Early Embryo Development ◽  
Nucleolar Stress

AbstractLIN28 is an RNA binding protein with important roles in early embryo development, stem cell differentiation/reprogramming, tumorigenesis and metabolism. Previous studies have focused mainly on its role in the cytosol where it interacts with Let-7 microRNA precursors or mRNAs, and few have addressed LIN28’s role within the nucleus. Here, we show that LIN28 displays dynamic temporal and spatial expression during murine embryo development. Maternal LIN28 expression drops upon exit from the 2-cell stage, and zygotic LIN28 protein is induced at the forming nucleolus during 4-cell to blastocyst stage development, to become dominantly expressed in the cytosol after implantation. In cultured pluripotent stem cells (PSCs), loss of LIN28 led to nucleolar stress and activation of a 2-cell/4-cell-like transcriptional program characterized by the expression of endogenous retrovirus genes. Mechanistically, LIN28 binds to small nucleolar RNAs and rRNA to maintain nucleolar integrity, and its loss leads to nucleolar phase separation defects, ribosomal stress and activation of P53 which in turn binds to and activates 2C transcription factor Dux. LIN28 also resides in a complex containing the nucleolar factor Nucleolin (NCL) and the transcriptional repressor TRIM28, and LIN28 loss leads to reduced occupancy of the NCL/TRIM28 complex on the Dux and rDNA loci, and thus de-repressed Dux and reduced rRNA expression. Lin28 knockout cells with nucleolar stress are more likely to assume a slowly cycling, translationally inert and anabolically inactive state, which is a part of previously unappreciated 2C-like transcriptional program. These findings elucidate novel roles for nucleolar LIN28 in PSCs, and a new mechanism linking 2C program and nucleolar functions in PSCs and early embryo development.

scholarly journals Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility

2016 ◽  
Vol 27 (5) ◽  
pp. 768-775 ◽  
Author(s):  
Xue-Shan Ma ◽  
Fei Lin ◽  
Zhong-Wei Wang ◽  
Meng-Wen Hu ◽  
Lin Huang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Primordial Follicle ◽  
Early Embryo ◽  
Cell Cycle Checkpoint ◽  
Low Fertility ◽  
Early Embryo Development ◽  
Damage Repair ◽  
Cycle Checkpoint ◽  
Oocyte Meiotic Maturation ◽  
Zygote Stage

Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in low fertility in mice. Even though there was no evident anomaly of oogenesis, oocyte meiotic maturation, natural ovulation, or fertilization, early embryo development and implantation were impaired. The fertilized eggs derived from mutant mice showed developmental delay, and many were blocked at the late zygote stage. Cdt1 protein was decreased, whereas Chk1 and H2AX phosphorylation was increased, in fertilized eggs after geminin depletion. Our results suggest that disruption of maternal geminin may decrease Cdt1 expression and cause DNA rereplication, which then activates the cell cycle checkpoint and DNA damage repair and thus impairs early embryo development.

scholarly journals The Potential Roles of the G1LEA and G3LEA Proteins in Early Embryo Development and in Response to Low Temperature and High Salinity in Artemia sinica

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0162272 ◽  
Author(s):  
Wei Zhao ◽  
Feng Yao ◽  
Mengchen Zhang ◽  
Ting Jing ◽  
Shuang Zhang ◽  
...  
Keyword(s):  
Low Temperature ◽  
Embryo Development ◽  
High Salinity ◽  
Early Embryo ◽  
Artemia Sinica ◽  
Early Embryo Development
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