scholarly journals Use of crystal violet solution for identifying the distal excision line during esophageal submucosal tunnel resection

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Hideki Kobara ◽  
Hirohito Mori ◽  
Tsutomu Masaki
Keyword(s):  
Crystal Violet ◽  
Crystal Violet Solution ◽  
Submucosal Tunnel

Efficacy of crystal violet for identifying the distal end in esophageal submucosal tunnel resection

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Hideki Kobara ◽  
Noriko Nishiyama ◽  
Hirohito Mori ◽  
Shintaro Fujihara ◽  
Tingting Shi ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Submucosal Tunnel

Decolorisation of aqueous crystal violet solution by a new nanoporous carbon: Equilibrium and kinetic approach

2014 ◽  
Vol 20 (5) ◽  
pp. 3375-3386 ◽  
Author(s):  
Fuat Güzel ◽  
Hasan Sayğılı ◽  
Gülbahar Akkaya Sayğılı ◽  
Filiz Koyuncu
Keyword(s):  
Crystal Violet ◽  
Nanoporous Carbon ◽  
Kinetic Approach ◽  
Crystal Violet Solution

scholarly journals Biofilm production by Listeria monocytogenes strains: detection with colorimetric analysis

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
P Centorame ◽  
L Iacone ◽  
R Salini ◽  
A Ciarulli ◽  
F Guidi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Colorimetric Analysis ◽  
Solution Versus ◽  
Crystal Violet Solution ◽  
Biofilm Production ◽  
Staining Methods

Abstract Background In literature, there are no standardized laboratory methods to detect formed biomass by colorimetric analysis. The purpose of this study was to compare three staining methods and two different wavelengths for determination of biofilm formation of Listeria monocytogenes (Lm) strains. Methods Three strains of Lm isolated from different origin were tested using 96 well polistirene plates at 12 °C and 30 °C, after incubation the wells were subjected to washing, detaching and staining with crystal violet (CV) at 0.2% and 2% (Panreac EU) in 95% ethanol and with Gram's crystal violet solution (Merck KGaA, Germany). The absorbance at 492nm and 540nm wavelengths was read using a spectrophotometer (SIRIO S, Seac, Firenze, Italia). Results The strains incubated at 12 °C displayed production of biofilm when stained with CV 2% and with Gram's crystal violet solution, both at 492 and 540 nm (with better evidence at 540 nm). If CV 0.2% was used to stain and reading at both optical densities there was evidence of weak or no biofilm production. At 30 °C, the biofilm production was displayed at both temperature and with all the stains. For all the strains and for all the conditions tested, the absorbance was greater but not proportional using the Gram's crystal violet solution, versus the CV 0,2% and CV 2%, and absorbance was higher at 540nm versus at 492nm. Conclusions Results confirmed the lack of reproducibility of each of the method used to detect and quantify the biomass produced during a biofilm formation test in vitro and the absence of ratio between the different results obtained using different CV concentration and wavelengths for reading. Key messages Biofilm production at 12 °C could not be adequately detected staining the wells with CV 0,2%. Absorbance could be influenced by the solvent in the stain used (ethanol, methanol or phenol or mixtures). To obtain data for assessment of biomass formation, being the method characterized by poor reproducibility, the laboratory should use at least the same stain and wavelength.

Properties of PbO2 Electrode Modified by AEO-7 for Electrocatalytic Degradation of Crystal Violet

2012 ◽  
Vol 550-553 ◽  
pp. 2029-2035
Author(s):  
Ying Ying Fan ◽  
Zhen Chen ◽  
Deng Hui Liao ◽  
Hong Quan Zhu ◽  
Qiang Lin
Keyword(s):  
Crystal Violet ◽  
Stainless Steel Substrate ◽  
Steel Substrate ◽  
Surface Active Agent ◽  
Active Agent ◽  
Cyclic Voltammograms ◽  
Crystal Violet Solution ◽  
Electrocatalytic Degradation ◽  
Acid Bath ◽  
Surface Active

In order to improve the performance of PbO2electrode, A kind of surface active agent – AEO-7 was added into nitric acid bath and modified PbO2electrode was prepared on stainless steel substrate by anodic oxidation. The modified variations on surface morphology of the electrodes were examined by Scanning Electron Microscopy(SEM); The polarization curves, Tafel curves and Cyclic voltammograms of modified and unmodified PbO2electrodes were compared in Crystal violet solution; The electrocatalytic activities were inspected by electrocatalytic degradation of crystal violet. It was found that the modified electrode showed higher oxygen evolution potential, better corrosion resistance, and more excellent electrocatalytic activity than the unmodified electrode.

MONITORING OF FORMATION OF BIOFILMS BY OPPORTUNISTIC AND PATHOGENIC BACTERIA

2019 ◽  
Vol 1 (2) ◽  
pp. 174-182
Author(s):  
A. B. Kononenko ◽  
◽  
D. A. Bannikova ◽  
S. V. Britova ◽  
I. B. Pavlova ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Filter Paper ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Room Temperature ◽  
Distilled Water ◽  
Pathogenic Microorganisms ◽  
Crystal Violet Solution ◽  
Taxonomic Groups ◽  
Peptone Broth

The aim of the work is monitoring the formation of biofilms by opportunistic and pathogenic microorganisms. Materials and methods. The cultures of the genera Salmonella, Escherichia, Yersinia, Proteus, Citrobacter, Enterobacter, Prtovidenzia, Morganella, Klebsiella, Cronobacter, Pseudomonas, Bacillus, Staphylococcus were used in the work. The studied microorganisms were cultured in polystyrene 96-well plates. For this purpose, a daily culture of microorganisms was introduced into the wells with meat-peptone broth, having previously established a concentration of 104 mc / ml, and incubated for 24...96 hours at temperature of 37 °C. Then the medium with plankton cells was removed from the wells. 200 μl of filtered 0,1% crystal violet solution was poured into the wells of the plate and the plates were kept for 10...15 min at room temperature. Then dye was removed from the wells. Unbound dye was thoroughly washed with saline or distilled water. The plates were turned over on filter paper and dried. The presence and density of biomatrix (biofilm) was determined visually by the intensity of staining the surfaces of plates. Then, for the extraction of paint from the film, 200 μl of 96% ethanol was added to the wells and the optical density was measured on KFK-3KM spectrophotometer at a wavelength of 590 nm. Results of research. The results of the experiments allowed us to assert that within 48 hours of cultivation microorganisms form a mature biofilm, which can serve as a model for studying the process of biofilm formation. Biofilm of microorganisms of different taxonomic groups differs in density. In addition, even bacteria belonging to the same genus, under the same conditions, can form a biofilm, the density of which differs by 30...60%.

On-Demand Guest Release from MOF-5 Sealed with Nitrophenylacetic Acid Photocapping Groups

2019 ◽  
Author(s):  
Jingjing Yan ◽  
Rick Homan ◽  
Corrianna Boucher ◽  
Prem N. Basa ◽  
Katherine Fossum ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Crystal Violet ◽  
Dye Molecules ◽  
Phenyl Acetic Acid ◽  
On Demand ◽  
Crystal Violet Dye ◽  
Dye Diffusion ◽  
Triphenylacetic Acid

Recently, we demonstrated that triphenylacetic acid could be used to seal dye molecules within MOF-5, but guest release required digestion of the framework by treatment with acid. We prepared the sterically bulky photocapping group [bis-(3-nitro-benzyl)-amino]-(3-nitro-phenyl)-acetic acid (PC1) can prevent Crystal violet dye diffusion from inside MOF-5 until removed by photolysis.

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