Inhibitory Effect of Yellow He-Ne Laser Irradiation Mediated by Crystal Violet Solution on Early Plaque Formation in Human Mouth

2000 ◽  
Vol 15 (3) ◽  
pp. 174-180 ◽  
Author(s):  
N. Senda ◽  
K. Ito ◽  
N. Sugano ◽  
Y. Moriya ◽  
K. Nanba ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Crystal Violet ◽  
Inhibitory Effect ◽  
Plaque Formation ◽  
Crystal Violet Solution

scholarly journals Effect of Zinc Salts on Respiratory Syncytial Virus Replication

2004 ◽  
Vol 48 (3) ◽  
pp. 783-790 ◽  
Author(s):  
Rahaman O. Suara ◽  
James E. Crowe
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Tract ◽  
Zinc Acetate ◽  
Lower Respiratory Tract ◽  
Divalent Cations ◽  
Cell Monolayer ◽  
Inhibitory Effect ◽  
Plaque Formation ◽  
Zinc Salts ◽  
Syncytial Virus

ABSTRACT Zinc supplementation decreases the morbidity of lower respiratory tract infection in pediatric patients in the developing world. We sought to determine if zinc mediates a specific inhibitory effect against the major cause of pediatric lower respiratory tract disease, respiratory syncytial virus (RSV). We determined the in vitro inhibitory effect of three zinc salts (zinc acetate, lactate, and sulfate) on the replication of RSV at various concentrations of 10 and 1 mM and 100 and 10 μM. The degree of inhibition of RSV replication was examined in the presence of zinc during preincubation, adsorption, or penetration and was compared with that caused by salts of other divalent cations. Complete inhibition of RSV plaque formation was observed at 1 and 10 mM, representing reductions that were ≥106-fold. At the lowest concentration tested, 10 μM, we observed ≥1,000-fold reductions in RSV yield when zinc was present during preincubation, adsorption, penetration, or egress of virus. The therapeutic indices, determined as ratios of 50% toxicity concentration to 50% inhibitory concentration, were 100, 150, and 120 for zinc acetate, zinc lactate, and zinc sulfate, respectively. The inhibitory effect of zinc salts on RSV was concentration dependent and was not observed with other salts containing divalent cations such as calcium, magnesium, and manganese. RSV plaque formation was prevented by pretreatment of HEp-2 cell monolayer cultures with zinc or by addition of zinc to methylcellulose overlay media after infection. The results of this study suggest that zinc mediates antiviral activity on RSV by altering the ability of the cell to support RSV replication.

scholarly journals The inhibitory effect of funoran and eucalyptus extract-containing chewing gum on plaque formation.

1998 ◽  
Vol 40 (3) ◽  
pp. 115-117 ◽  
Author(s):  
Shuichi Sato ◽  
Naoto Yoshinuma ◽  
Koichi Ito ◽  
Takumi Tokumoto ◽  
Toshio Takiguchi ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Plaque Formation ◽  
Chewing Gum

scholarly journals Inhibitory Effect of Various Mouthrinses on de novo Plaque Formation.

2001 ◽  
Vol 43 (3) ◽  
pp. 283-288
Author(s):  
Satoshi Sekino ◽  
Reiko Aiba ◽  
Toshifumi Aiba ◽  
Takenori Tsukahara ◽  
Toshio Tashiro ◽  
...  
Keyword(s):  
De Novo ◽  
Inhibitory Effect ◽  
Plaque Formation

scholarly journals Antibodies against dextransucrase from Streptococcus mutans display anti-biofilm and growth suppressing activities

2021 ◽  
Author(s):  
Shabeer Ahmad Rather ◽  
Akhtar Mahmood ◽  
Lakhvinder Singh ◽  
Alka Bhatia ◽  
Sukesh Chander Sharma ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Membrane Fraction ◽  
Inhibitory Effect ◽  
Cross Reactivity ◽  
Plaque Formation ◽  
Hydrophobic Properties ◽  
Experimental Conditions ◽  
Glass Surfaces ◽  
Reduced Adherence

Abstract Dextransucrase produced by Streptococcus mutans play an essential role in the formation of dental caries by synthesizing exopolysaccharides from sucrose, an important metabolite of the organism. In this study we report the location of dextransucrase in Streptococcus mutans cells and describe that antibodies raised against dextransucrase inhibited biofilm formation and reduced the adherence and hydrophobic properties of Streptococcus mutans. Western blot analysis and immunoelectron microscopy revealed that dextransucrase is located abundantly in the membrane fraction in S. mutans cells. Scanning electron microscopy and fluorescence microscopy revealed reduced cell density, impaired bioflim (plaque) formation in presence of dextransucrase antibodies. Genes associated with bioflim formation in S. mutans such as GtfB, GtfC, BrpA, relA, Smu630, vicK were down regulated (50–97%) in presence of the enzyme antibody. Presence of enzyme antibodies reduced adherence of S. mutans cells to glass surfaces by 58% and hydrophobicity by 55.2%. However dextransucrase antibodies did not affect acid production by S. mutans, under the experimental conditions. Immunohistochemistry studies with certain human samples displayed no cross reactivity with dextransucrase antibody. These findings suggest that antibodies against dextransucrase exhibit a profound inhibitory effect on the vital cariogenic factors of S. mutans and have no cross reactivity with human tissues tested, thus implying that dextransucrase could be a promising antigen to study its anticariogenic potential.

Decolorisation of aqueous crystal violet solution by a new nanoporous carbon: Equilibrium and kinetic approach

2014 ◽  
Vol 20 (5) ◽  
pp. 3375-3386 ◽  
Author(s):  
Fuat Güzel ◽  
Hasan Sayğılı ◽  
Gülbahar Akkaya Sayğılı ◽  
Filiz Koyuncu
Keyword(s):  
Crystal Violet ◽  
Nanoporous Carbon ◽  
Kinetic Approach ◽  
Crystal Violet Solution
Sign in / Sign up

Export Citation Format

Share Document