scholarly journals Hydrogen sulfide donor protects against mechanical ventilation‐induced atrophy and contractile dysfunction in the rat diaphragm

2021 ◽  
Author(s):  
Noriko Ichinoseki‐Sekine ◽  
Ashley J. Smuder ◽  
Aaron B. Morton ◽  
J. Matthew Hinkley ◽  
Andres Mor Huertas ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Hydrogen Sulfide ◽  
Contractile Dysfunction ◽  
Rat Diaphragm

Effect of hydrogen sulfide on the characteristics of the neuromuscular synapse terminals of the rat diaphragm

2021 ◽  
Author(s):  
A.N. Kadenov ◽  
O.V. Yakovleva
Keyword(s):  
Hydrogen Sulfide ◽  
Synaptic Transmission ◽  
Key Words ◽  
Fluorescent Microscopy ◽  
Neuromuscular Synapse ◽  
Antioxidant Property ◽  
Postnatal Ontogenesis ◽  
Nerve Terminals ◽  
Rat Diaphragm ◽  
Biological Functions

Hydrogen sulfide is one of the gas-transmitters that also performs other biological functions. The antioxidant property of this substance is one of the important ones. The research was conducted on rats of both sexes between 6 and 18 days of age. We have shown that the offspring of females injected subcutaneously with hydrogen sulfide increased the area and luminescence of nerve terminals during postnatal ontogenesis, which can be further used to level the effects of hyperhomocysteinemia on synaptic transmission. Key words: neuromuscular synapse, fluorescent microscopy, hydrogen sulfide.

scholarly journals Early Changes in Rat Diaphragm Biology with Mechanical Ventilation

2003 ◽  
Vol 168 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Gábor Z. Rácz ◽  
Ghislaine Gayan-Ramirez ◽  
Dries Testelmans ◽  
Pascal Cadot ◽  
Kristel De Paepe ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Rat Diaphragm

scholarly journals Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction

2009 ◽  
Vol 106 (2) ◽  
pp. 385-394 ◽  
Author(s):  
Melissa A. Whidden ◽  
Joseph M. McClung ◽  
Darin J. Falk ◽  
Matthew B. Hudson ◽  
Ashley J. Smuder ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mechanical Ventilation ◽  
Xanthine Oxidase ◽  
Prolonged Mechanical Ventilation ◽  
Oxidative Injury ◽  
Contractile Dysfunction ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Oxidant Production ◽  
Diaphragmatic Weakness

Respiratory muscle weakness resulting from both diaphragmatic contractile dysfunction and atrophy has been hypothesized to contribute to the weaning difficulties associated with prolonged mechanical ventilation (MV). While it is clear that oxidative injury contributes to MV-induced diaphragmatic weakness, the source(s) of oxidants in the diaphragm during MV remain unknown. These experiments tested the hypothesis that xanthine oxidase (XO) contributes to MV-induced oxidant production in the rat diaphragm and that oxypurinol, a XO inhibitor, would attenuate MV-induced diaphragmatic oxidative stress, contractile dysfunction, and atrophy. Adult female Sprague-Dawley rats were randomly assigned to one of six experimental groups: 1) control, 2) control with oxypurinol, 3) 12 h of MV, 4) 12 h of MV with oxypurinol, 5) 18 h of MV, or 6) 18 h of MV with oxypurinol. XO activity was significantly elevated in the diaphragm after MV, and oxypurinol administration inhibited this activity and provided protection against MV-induced oxidative stress and contractile dysfunction. Specifically, oxypurinol treatment partially attenuated both protein oxidation and lipid peroxidation in the diaphragm during MV. Further, XO inhibition retarded MV-induced diaphragmatic contractile dysfunction at stimulation frequencies >60 Hz. Collectively, these results suggest that oxidant production by XO contributes to MV-induced oxidative injury and contractile dysfunction in the diaphragm. Nonetheless, the failure of XO inhibition to completely prevent MV-induced diaphragmatic oxidative damage suggests that other sources of oxidant production are active in the diaphragm during prolonged MV.

scholarly journals Oxidants Regulated Diaphragm Proteolysis during Mechanical Ventilation in Rats

2019 ◽  
Vol 131 (3) ◽  
pp. 605-618 ◽  
Author(s):  
Nikolay Moroz ◽  
Karen Maes ◽  
Jean-Philippe Leduc-Gaudet ◽  
Peter Goldberg ◽  
Basil J. Petrof ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mechanical Ventilation ◽  
Quantitative Polymerase Chain Reaction ◽  
Male Rats ◽  
Contractile Dysfunction ◽  
E3 Ligases ◽  
Protein Ubiquitination ◽  
Pathway Activation ◽  
Controlled Mechanical Ventilation ◽  
Fiber Atrophy

Abstract Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New Background Diaphragm dysfunction and atrophy develop during prolonged controlled mechanical ventilation. Fiber atrophy has been attributed to activation of the proteasome and autophagy proteolytic pathways. Oxidative stress activates the proteasome during controlled mechanical ventilation, but it is unclear whether it also activates autophagy. This study investigated whether pretreatment with the antioxidant N-acetylcysteine affects controlled mechanical ventilation–induced diaphragm contractile dysfunction, fiber atrophy, and proteasomal and autophagic pathway activation. The study also explored whether proteolytic pathway activity during controlled mechanical ventilation is mediated by microRNAs that negatively regulate ubiquitin E3 ligases and autophagy-related genes. Methods Three groups of adult male rats were studied (n = 10 per group). The animals in the first group were anesthetized and allowed to spontaneously breathe. Animals in the second group were pretreated with saline before undergoing controlled mechanical ventilation for 24 h. The animals in the third group were pretreated with N-acetylcysteine (150 mg/kg) before undergoing controlled mechanical ventilation for 24 h. Diaphragm contractility and activation of the proteasome and autophagy pathways were measured. Expressions of microRNAs that negatively regulate ubiquitin E3 ligases and autophagy-related genes were measured with quantitative polymerase chain reaction. Results Controlled mechanical ventilation decreased diaphragm twitch force from 428 ± 104 g/cm2 (mean ± SD) to 313 ± 50 g/cm2 and tetanic force from 2,491 ± 411 g/cm2 to 1,618 ± 177 g/cm2. Controlled mechanical ventilation also decreased diaphragm fiber size, increased expression of several autophagy genes, and augmented Atrogin-1, MuRF1, and Nedd4 expressions by 36-, 41-, and 8-fold, respectively. Controlled mechanical ventilation decreased the expressions of six microRNAs (miR-20a, miR-106b, miR-376, miR-101a, miR-204, and miR-93) that regulate autophagy genes. Pretreatment with N-acetylcysteine prevented diaphragm contractile dysfunction, attenuated protein ubiquitination, and downregulated E3 ligase and autophagy gene expression. It also reversed controlled mechanical ventilation–induced microRNA expression decreases. N-Acetylcysteine pretreatment had no affect on fiber atrophy. Conclusions Prolonged controlled mechanical ventilation activates the proteasome and autophagy pathways in the diaphragm through oxidative stress. Pathway activation is accomplished, in part, through inhibition of microRNAs that negatively regulate autophagy-related genes.

scholarly journals Mechanical Ventilation Depresses Protein Synthesis in the Rat Diaphragm

2004 ◽  
Vol 170 (9) ◽  
pp. 994-999 ◽  
Author(s):  
R. Andrew Shanely ◽  
Darin Van Gammeren ◽  
Keith C. DeRuisseau ◽  
A. Murat Zergeroglu ◽  
Michael J. McKenzie ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Protein Synthesis ◽  
Rat Diaphragm

scholarly journals Mechanical ventilation impairs sarcomeric protein function in rat diaphragm single fibers

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Bumsoo S. Ahn ◽  
Kurt J. Sollanek ◽  
Ashley J. Smuder ◽  
Michael P. Wiggs ◽  
Scott K. Powers ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Protein Function ◽  
Rat Diaphragm ◽  
Single Fibers ◽  
Sarcomeric Protein

scholarly journals Prolonged controlled mechanical ventilation in humans triggers myofibrillar contractile dysfunction and myofilament protein loss in the diaphragm

Thorax ◽  
2016 ◽  
Vol 71 (5) ◽  
pp. 436-445 ◽  
Author(s):  
Sabah N A Hussain ◽  
Anabelle S Cornachione ◽  
Céline Guichon ◽  
Auday Al Khunaizi ◽  
Felipe de Souza Leite ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Contractile Dysfunction ◽  
Protein Loss ◽  
Controlled Mechanical Ventilation
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