scholarly journals Comprehensive, Multimodal Characterization of an Imiquimod-Induced Human Skin Inflammation Model for Drug Development

2018 ◽  
Vol 11 (6) ◽  
pp. 607-615 ◽  
Author(s):  
Tessa van der Kolk ◽  
Salma Assil ◽  
Rianne Rijneveld ◽  
Erica S. Klaassen ◽  
Gary Feiss ◽  
...  
Keyword(s):  
Drug Development ◽  
Human Skin ◽  
Skin Inflammation ◽  
Inflammation Model

scholarly journals Development and Evaluation of a Topical Anti-Inflammatory Preparation Containing Dodonaea polyandra Extract

2015 ◽  
Vol 18 (4) ◽  
pp. 578 ◽  
Author(s):  
Bradley S Simpson ◽  
Xianling Luo ◽  
Jiping Wang ◽  
Yunmei Song ◽  
David Claudie ◽  
...  
Keyword(s):  
Phase Separation ◽  
Human Skin ◽  
Dosage Form ◽  
Interleukin 1 ◽  
In Vitro Release ◽  
Skin Inflammation ◽  
Skin Model ◽  
Inflammation Model ◽  
Anti Inflammatory

Purpose: We have previously reported that the Australian Northern Kaanju (Kuuku I’yu) medicinal plant Dodonaea polyandra has anti-inflammatory activity. This is attributed largely to the presence of clerodane diterpenoids contained within the leaf resin. We envisaged developing a topical preparation to treat indications relating to skin inflammation. However, it was unknown whether the resin could be incorporated into a suitable dosage form while retaining the therapeutic value demonstrated in previous work. Therefore, the following study was undertaken to assess parameters of safety and efficacy for a prototype formulation containing the leaf resin extracted from D. polyandra. Methods: Using the assessment criteria of optimum appearance, tactile feeling, spreadability and odour, 78 different formulations were developed. Formulation stability was assessed using a centrifugal test with preparations displaying phase separation further modified or re-formulated. A prototype formulation containing 5% w/w plant resin was selected and subjected to in vitro release studies. This was quantified through HPLC analysis using two major bioactive diterpenoids as reference. The prototype formulation was tested for efficacy in a TPA-induced acute murine skin inflammation model as well as a 3D human skin model for irritancy/toxicity (Epiderm™). Results: The prototype resin cream was a chartreuse-coloured homogenous semisolid preparation that was readily spreadable upon contact with skin with no sensation of tackiness, residual greasiness, or irritation. The optimized cream showed no phase separation after 30 min centrifugation at 825 g. In the TPA-induced inflammation model, the resin formulation significantly reduced ear thickness and interleukin-1 beta levels in mouse ear tissue. The 5% w/w resin cream formulation showed no irritancy in a 3D human skin model. Conclusions: Our results demonstrate that bioactive resin from D. polyandra can be formulated into a stable and non-irritant semi-solid dosage form and reduce parameters of acute skin inflammation in vivo. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Cellular and Biochemical Characterization of the Anti-Inflammatory Effects of DuP 654 in the Arachidonic Acid Murine Skin Inflammation Model (Part 1 of 2)

1990 ◽  
Vol 3 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Richard R. Harris ◽  
William M. Mackin ◽  
Douglas G. Batt ◽  
Suzanne M. Rakich ◽  
Robert J. Collins ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Biochemical Characterization ◽  
Skin Inflammation ◽  
Inflammation Model ◽  
Anti Inflammatory

scholarly journals 670 Characterization of a novel IL-36-induced skin inflammation model in mice

2017 ◽  
Vol 137 (5) ◽  
pp. S115
Author(s):  
Z. Su ◽  
J. Wetter ◽  
K. Salte ◽  
R. Edelmayer ◽  
A. Kannan ◽  
...  
Keyword(s):  
Skin Inflammation ◽  
Inflammation Model

Cellular and Biochemical Characterization of the Anti-Inflammatory Effects of DuP 654 in the Arachidonic Acid Murine Skin Inflammation Model (Part 2 of 2)

1990 ◽  
Vol 3 (1) ◽  
pp. 35-40
Author(s):  
Richard R. Harris ◽  
William M. Mackin ◽  
Douglas G. Bait ◽  
Suzanne M. Rakich ◽  
Robert J. Collins ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Biochemical Characterization ◽  
Skin Inflammation ◽  
Inflammation Model ◽  
Anti Inflammatory

scholarly journals Development and characterization of a human Th17‐driven ex vivo skin inflammation model

2020 ◽  
Vol 29 (10) ◽  
pp. 993-1003
Author(s):  
Claire Jardet ◽  
Anthony David ◽  
Emilie Braun ◽  
Pascal Descargues ◽  
Jean‐Louis Grolleau ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Skin Inflammation ◽  
Inflammation Model

scholarly journals Creation of Ionically Crosslinked Tri-Layered Chitosan Membranes to Simulate Different Human Skin Properties

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1807
Author(s):  
Rocío Guerle-Cavero ◽  
Blanca Lleal-Fontàs ◽  
Albert Balfagón-Costa
Keyword(s):  
Elastic Modulus ◽  
Human Skin ◽  
Sodium Tripolyphosphate ◽  
Snow Crab ◽  
Suitable Material ◽  
Cosmetic Industry ◽  
Novel Method ◽  
Current Article ◽  
Chitosan Membranes

In 2023, new legislation will ban the use of animals in the cosmetic industry worldwide. This fact, together with ethical considerations concerning the use of animals or humans in scientific research, highlights the need to propose new alternatives for replacing their use. The aim of this study is to create a tri-layered chitosan membrane ionically crosslinked with sodium tripolyphosphate (TPP) in order to simulate the number of layers in human skin. The current article highlights the creation of a membrane where pores were induced by a novel method. Swelling index, pore creation, and mechanical property measurements revealed that the swelling index of chitosan membranes decreased and, their pore formation and elasticity increased with an increase in the Deacetylation Grade (DDA). Additionally, the results demonstrate that chitosan’s origin can influence the elastic modulus value and reproducibility, with higher values being obtained with seashell than snow crab or shrimp shells. Furthermore, the data show that the addition of each layer, until reaching three layers, increases the elastic modulus. Moreover, if layers are crosslinked, the elastic modulus increases to a much greater extent. The characterization of three kinds of chitosan membranes was performed to find the most suitable material for studying different human skin properties.

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Biopolymers ◽  
2007 ◽  
Vol 87 (4) ◽  
pp. 261-274 ◽  
Author(s):  
Ali Tfayli ◽  
Olivier Piot ◽  
Florence Draux ◽  
Franck Pitre ◽  
Michel Manfait
Keyword(s):  
Molecular Characterization ◽  
Human Skin ◽  
Raman Microspectroscopy ◽  
Skin Model ◽  
Reconstructed Skin

scholarly journals Molecular Characterization of Human Skin Response to Diphencyprone at Peak and Resolution Phases: Therapeutic Insights

2014 ◽  
Vol 134 (10) ◽  
pp. 2531-2540 ◽  
Author(s):  
Nicholas Gulati ◽  
Mayte Suárez-Fariñas ◽  
Judilyn Fuentes-Duculan ◽  
Patricia Gilleaudeau ◽  
Mary Sullivan-Whalen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Human Skin ◽  
Skin Response
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