Prevalence, determinants, and correlates of coagulation necrosis and contraction band necrosis in donor hearts

2019 ◽  
Vol 33 (2) ◽  
pp. e13472 ◽  
Author(s):  
Maya Guglin ◽  
Hesham R. Omar ◽  
Gregory Ray ◽  
Charles Wright
Keyword(s):  
Coagulation Necrosis ◽  
Contraction Band Necrosis ◽  
Contraction Band

Abstract P502: Abnormal Myocyte Calcium Dynamics And The Resultant Contraction Band Formation Of The Rat Heart Under Irreversible Injury By Membrane Permeabilization

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Yuma Morishita ◽  
Shoko Tamura ◽  
Kentaro Mochizuki ◽  
Yoshinori Harada ◽  
Hideo Tanaka
Keyword(s):  
Fluorescence Intensity ◽  
High Frequency ◽  
Time Course ◽  
Morphological Changes ◽  
Confocal Imaging ◽  
Band Formation ◽  
Contraction Band Necrosis ◽  
Scanning Confocal Microscopy ◽  
The Individual ◽  
Contraction Band

Ca 2+ overload is a cardinal feature of cardiomyocyte injury, and its progression to irreversible state leads to cell death. However, unknowns are the precise spatiotemporal changes in the myocyte Ca 2+ dynamics and the relevant cell morphology of irreversibly injured hearts. On the hypothesis that myocytes exhibit high-frequency Ca 2+ waves and contraction band necrosis in saponin-permeabilized injured heart, we observed changes in the Ca 2+ dynamics and the relevant morphological changes in the subepicardial myocardium of the Fluo4-loaded rat hearts (n = 14) by rapid-scanning confocal microscopy (100 frames/s) under Langendorff perfusion with 0.3 mM Ca 2+ -Tyrode solution including 0.4 % saponin at 30°C. Also performed was confocal imaging of tetramethylrhodamine methyl ester (TMRM) fluorescence of the myocardium. Under quasi-quiescence of the heart after dissection of the SA node, individual myocytes barely exhibited spontaneous Ca 2+ waves, whereas after commencement of saponin perfusion high-frequency (118 ± 9.7 /min/cell, mean ± SEM) Ca 2+ waves (hereafter, “agonal waves”) emerged within 1 min, showing asynchronous, oscillatory contractions in the individual myocytes with a V prop of 124 ± 2.5 μm/s (n = 60). Subsequently, the waves gradually decreased in frequency with concomitant slowing of its decay time course, and eventually, disappeared in 6 min; myocytes exhibited high, static Fluo4-fluorescence intensity. Along with the progression of Ca 2+ overload by saponin, the TMRM fluorescence intensity was discretely lost in individual myocytes. The myocytes showing the agonal waves exhibited contraction bands, i.e., band-like aggregations of the actin fibers. Under mechanical arrest of the heart by 2,3-butanedione monoxime (20 mM), saponin still induced the agonal waves with a frequency of 253 ± 10.6 /cell/min and V prop of 118 ± 2.1 μm/s (n = 60); however, contraction bands were barely seen.In conclusion, irreversible myocyte injury by saponin provoked agonal Ca 2+ waves and oscillatory contractions indicating progressive Ca 2+ overload and the following mitochondrial damage, which may provide deeper insights into understanding the mechanism of contraction band necrosis.

Contraction-band necrosis: new use for an old friend

The Lancet ◽  
1996 ◽  
Vol 347 (9017) ◽  
pp. 1710-1711 ◽  
Author(s):  
Renu Virmani ◽  
Andrew Farb ◽  
Allen Burke
Keyword(s):  
Contraction Band Necrosis ◽  
Contraction Band

Frequency and extent of contraction band necrosis in orthotopically transplanted human hearts. A morphometric study

2003 ◽  
Vol 88 (2-3) ◽  
pp. 267-278 ◽  
Author(s):  
Giorgio Baroldi ◽  
Malcom D Silver ◽  
Renata De Maria ◽  
Marina Parolini ◽  
Emanuela Turillazzi ◽  
...  
Keyword(s):  
Morphometric Study ◽  
Contraction Band Necrosis ◽  
Contraction Band

scholarly journals Quantitative analysis of contraction band and coagulation necrosis after ischemia and reperfusion in the porcine heart.

Circulation ◽  
1987 ◽  
Vol 75 (5) ◽  
pp. 1074-1082 ◽  
Author(s):  
S Miyazaki ◽  
H Fujiwara ◽  
T Onodera ◽  
Y Kihara ◽  
M Matsuda ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Coagulation Necrosis ◽  
Ischemia And Reperfusion ◽  
Porcine Heart ◽  
Contraction Band
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