scholarly journals Cellular behaviours of bone marrow-derived mesenchymal stem cells towards pristine graphene oxide nanosheets

2017 ◽  
Vol 50 (5) ◽  
pp. e12367 ◽  
Author(s):  
Changbo Wei ◽  
Zifeng Liu ◽  
Fangfang Jiang ◽  
Binghui Zeng ◽  
Mingdi Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Pristine Graphene ◽  
Graphene Oxide Nanosheets

Low-dose suspended graphene oxide nanosheets induce antioxidant response and osteogenic differentiation of bone marrow-derived mesenchymal stem cells via JNK-dependent FoxO1 activation

2019 ◽  
Vol 7 (39) ◽  
pp. 5998-6009 ◽  
Author(s):  
Alexander Halim ◽  
Lin Liu ◽  
Agnes Dwi Ariyanti ◽  
Yang Ju ◽  
Qing Luo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Low Dose ◽  
Antioxidant Response ◽  
Graphene Oxide Nanosheets ◽  
Suspended Graphene

Low-dose GO nanosheets enhance the antioxidant response and facilitate osteogenic differentiation of bone marrow-derived mesenchymal stem cells through the JNK-FoxO1 pathways.

Reduced graphene oxide facilitates biocompatibility of alginate for cardiac repair

2020 ◽  
Vol 35 (4-5) ◽  
pp. 363-377
Author(s):  
Negar Karimi Hajishoreh ◽  
Nafiseh Baheiraei ◽  
Nasim Naderi ◽  
Mojdeh Salehnia
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Tissue Culture ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Physicochemical Properties ◽  
Reduced Graphene Oxide ◽  
Cardiac Repair ◽  
Reduced Graphene ◽  
Tissue Culture Plate

The benefits of combined cell/material therapy appear promising for myocardial infarction treatment. The safety of alginate, along with its excellent biocompatibility and biodegradability, has been extensively investigated for cardiac tissue engineering. Among graphene-based nanomaterials, reduced graphene oxide has been considered as a promising candidate for cardiac treatment due to its unique physicochemical properties. In this study, the reduced graphene oxide incorporation effect within alginate hydrogels was investigated for cardiac repair application. Reduced graphene oxide reinforced alginate properties, resulting in an increase in gel stiffness. The cytocompatibility of the hydrogels prepared with human bone marrow–derived mesenchymal stem cells was assessed by the 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay. Following reduced graphene oxide addition, alginate-reduced graphene oxide retained significantly higher cell viability compared to that of alginate and cells cultured on tissue culture plates. Acridine orange/propidium iodide staining was also used to identify both viable and necrotic human bone marrow–derived mesenchymal stem cells within the prepared hydrogels. After a 72-h culture, the percentage of viable cells was twice as much as those cultured on either alginate or tissue culture plate, reaching approximately 80%. Quantitative reverse transcription polymerase chain reaction analysis was performed to assess gene expression of neonatal rat cardiac cells encapsulated on hydrogels for TrpT-2, Conx43, and Actn4 after 7 days. The expression of all genes in alginate-reduced graphene oxide increased significantly compared to that in alginate or tissue culture plate. The results obtained confirmed that the presence of reduced graphene oxide, as an electro-active moiety within alginate, could tune the physicochemical properties of this material, providing a desirable electroactive hydrogel for stem cell therapy in patients with ischemic heart disease.

Self – aligned mesoporous titania nanotubes – reduced graphene oxide hybrid surface: A potential scaffold for osteogenesis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shikha Wadhwa ◽  
Souradeep Roy ◽  
Neha Mittal ◽  
Alishba T John ◽  
Swati Midha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Bone Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Reduced Graphene ◽  
Marrow Mesenchymal Stem Cells

Abstract Damaged or degenerative joints are treated with total joint replacement, however, despite many successful outcomes, significant problems such as implant loosening, and failure occur. These problems occur due to the inability of the implant to support bone cell attachment, delaying the cell–implant interaction affecting bone regeneration. Hence it is recommended to develop and modify implants to induce bone cell proliferation. Herein, we report a self-aligned titania nanotubes–reduced graphene oxide (TiNT–reduced GO) hybrid surface for growth and proliferation of human bone marrow mesenchymal stem cells. The hybrid surface is characterised using electron microscopy, X-ray diffraction and Raman spectroscopy. The human bone marrow mesenchymal stem cell proliferation was studied using standard MTT and fluorescence assays. TiNT–reduced GO hybrid surface, with TiO2 nanotube of diameter 80 –110 nm and length ⁓500 μm with uniform deposition of reduced GO all over the surface, demonstrated about 50% increased cell growth compared to TiO2 nanotubes surface. The osteoinductive behaviour of TiNT–reduced GO is observed as these hybrid films support the proliferation of human bone marrow mesenchymal stem cells, hence promising to be a potential implant material for bone regeneration.

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