scholarly journals Protein kinase A inhibitor, H89, significantly enhances survival rate of dissociated human embryonic stem cells following cryopreservation

2016 ◽  
Vol 49 (5) ◽  
pp. 589-598 ◽  
Author(s):  
Liang Zhang ◽  
Yanqing Xu ◽  
Jiandong Xu ◽  
Yuping Wei ◽  
Xia Xu
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Kinase A

scholarly journals Protein Kinase C Mediated Extraembryonic Endoderm Differentiation of Human Embryonic Stem Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (3) ◽  
pp. 461-470 ◽  
Author(s):  
Xuezhu Feng ◽  
Jiuchun Zhang ◽  
Kimberly Smuga-Otto ◽  
Shulan Tian ◽  
Junying Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Endoderm Differentiation ◽  
Extraembryonic Endoderm ◽  
Kinase C

Caspase Inhibitor Z-VAD-FMK Enhances the Freeze-Thaw Survival Rate of Human Embryonic Stem Cells

2007 ◽  
Vol 27 (4-5) ◽  
pp. 257-264 ◽  
Author(s):  
Boon Chin Heng ◽  
Marie Veronique Clement ◽  
Tong Cao
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Culture Media ◽  
Embryonic Stem ◽  
Immunocytochemical Staining ◽  
Irreversible Inhibitor ◽  
Slow Cooling ◽  
Spontaneous Differentiation

Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence, this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes, Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 100 mM Z-VAD-FMK was supplemented into either the freezing solution, the post-thaw culture media or both. Intact and adherent hESC colonies were cryopreserved so as to enable subsequent quantitation of the post-thaw cell survival rate through the MTT assay, which can only be performed with adherent cells. Exposure to 100 mM Z-VAD-FMK in the freezing solution alone did not significantly enhance the post-thaw survival rate (10.2% vs. 9.9%, p > 0.05). However, when 100 mM Z-VAD-FMK was added to the post-thaw culture media, there was a significant enhancement in the survival rate from 9.9% to 14.4% (p < 0.05), which was further increased to 18.7% when Z-VAD-FMK was also added to the freezing solution as well (p < 0.01). Spontaneous differentiation of hESC after cryopreservation was assessed by morphological observations under bright-field microscopy, and by immunocytochemical staining for the pluripotency markers SSEA-3 and TRA-1-81. The results demonstrated that exposure to Z-VAD-FMK did not significantly enhance the spontaneous differentiation of hESC within post-thaw culture.

The ROCK Inhibitor Y-27632 Enhances the Survival Rate of Human Embryonic Stem Cells Following Cryopreservation

2008 ◽  
Vol 17 (6) ◽  
pp. 1079-1086 ◽  
Author(s):  
Xiangyun Li ◽  
Guoliang Meng ◽  
Roman Krawetz ◽  
Shiying Liu ◽  
Derrick E. Rancourt
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Rock Inhibitor

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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