scholarly journals FGF ‐2 addition during expansion of human bone marrow‐derived stromal cells alters MSC surface marker distribution and chondrogenic differentiation potential

2013 ◽  
Vol 46 (4) ◽  
pp. 396-407 ◽  
Author(s):  
S. Hagmann ◽  
B. Moradi ◽  
S. Frank ◽  
T. Dreher ◽  
P. W. Kämmerer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Surface Marker ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Marker Distribution

scholarly journals Osteoporosis is accompanied by reduced CD274 expression in human bone marrow-derived mesenchymal stem cells

2021 ◽  
Vol 41 ◽  
pp. 603-615
Author(s):  
A-N Zeller ◽  
◽  
M Selle ◽  
Z Gong ◽  
M Winkelmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Chondrogenic Differentiation ◽  
Surface Antigens ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Proliferation Potential

Underlying pathomechanisms of osteoporosis are still not fully elucidated. Cell-based therapy approaches pose new possibilities to treat osteoporosis and its complications. The aim of this study was to quantify differences in human bone marrow-derived mesenchymal stem cells (hBMSCs) between healthy donors and those suffering from clinically manifest osteoporosis. Cell samples of seven donors for each group were selected retrospectively from the hBMSC cell bank of the Trauma Department of Hannover Medical School. Cells were evaluated for their adipogenic, osteogenic and chondrogenic differentiation potential, for their proliferation potential and expression of surface antigens. Furthermore, a RT2 Osteoporosis Profiler PCR array, as well as quantitative real-time PCR were carried out to evaluate changes in gene expression. Cultivated hBMSCs from osteoporotic donors showed significantly lower cell surface expression of CD274 (4.98 % ± 2.38 %) than those from the control group (26.03 % ± 13.39 %; p = 0.007), as assessed by flow cytometry. In osteoporotic patients, genes involved in inhibition of the anabolic WNT signalling pathway and those associated with stimulation of bone resorption were significantly upregulated. Apart from these changes, no significant differences were found for the other cell surface antigens, adipogenic, osteogenic and chondrogenic differentiation ability as well as proliferation potential. These findings supported the theory of an influence of CD274 on the regulation of bone metabolism. CD274 might be a promising target for further investigations of the pathogenesis of osteoporosis and of cell-based therapies involving MSCs.

scholarly journals Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Ute Hempel ◽  
Katrin Müller ◽  
Carolin Preissler ◽  
Carolin Noack ◽  
Sabine Boxberger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Peroxisome Proliferator ◽  
Differentiation Potential

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.

scholarly journals Fate of tenogenic differentiation potential of human bone marrow stromal cells by uniaxial stretching affected by stretch-activated calcium channel agonist gadolinium

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178117 ◽  
Author(s):  
Hui Yin Nam ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Belinda Pingguan-Murphy ◽  
Azlina A. Abbas ◽  
Azhar M. Merican ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Channel ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Uniaxial Stretching ◽  
Tenogenic Differentiation

scholarly journals Chondrogenic differentiation of human bone marrow‐derived mesenchymal stromal cells in a three‐dimensional environment

2019 ◽  
Vol 235 (4) ◽  
pp. 3497-3507
Author(s):  
Eve Salonius ◽  
Leena Kontturi ◽  
Anita Laitinen ◽  
Anne‐Marie Haaparanta ◽  
Matti Korhonen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow

Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells

2000 ◽  
Vol 28 (6) ◽  
pp. 707-715 ◽  
Author(s):  
Andrea Banfi ◽  
Anita Muraglia ◽  
Beatrice Dozin ◽  
Maddalena Mastrogiacomo ◽  
Ranieri Cancedda ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Ex Vivo ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Proliferation Kinetics
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