Comparison of phenotypic markers and neural differentiation potential of human bone marrow stromal cells from the cranial bone and iliac crest

2019 ◽  
Vol 234 (9) ◽  
pp. 15235-15242
Author(s):  
Yuyuan Ma ◽  
Jie Ma ◽  
Yuanyuan Zhao ◽  
Kaichuang Yang ◽  
Jia Zhou ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Neural Differentiation ◽  
Iliac Crest ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cranial Bone ◽  
Differentiation Potential

scholarly journals Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Ute Hempel ◽  
Katrin Müller ◽  
Carolin Preissler ◽  
Carolin Noack ◽  
Sabine Boxberger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Peroxisome Proliferator ◽  
Differentiation Potential

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.

scholarly journals Fate of tenogenic differentiation potential of human bone marrow stromal cells by uniaxial stretching affected by stretch-activated calcium channel agonist gadolinium

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178117 ◽  
Author(s):  
Hui Yin Nam ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Belinda Pingguan-Murphy ◽  
Azlina A. Abbas ◽  
Azhar M. Merican ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Channel ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Uniaxial Stretching ◽  
Tenogenic Differentiation

Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals

Bone ◽  
2006 ◽  
Vol 38 (6) ◽  
pp. 758-768 ◽  
Author(s):  
Sunday O. Akintoye ◽  
Thanh Lam ◽  
Songtao Shi ◽  
Jaime Brahim ◽  
Michael T. Collins ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Iliac Crest ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Site Specific ◽  
Skeletal Site

Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells

2000 ◽  
Vol 28 (6) ◽  
pp. 707-715 ◽  
Author(s):  
Andrea Banfi ◽  
Anita Muraglia ◽  
Beatrice Dozin ◽  
Maddalena Mastrogiacomo ◽  
Ranieri Cancedda ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Ex Vivo ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Proliferation Kinetics

scholarly journals Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium: a pilot study

2008 ◽  
Vol 19 (11) ◽  
pp. 1197-1201 ◽  
Author(s):  
Sunday O. Akintoye ◽  
Parascevi Giavis ◽  
Derek Stefanik ◽  
Lawrence Levin ◽  
Francis K. Mante
Keyword(s):  
Bone Marrow ◽  
Pilot Study ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Iliac Crest ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model

2000 ◽  
Vol 113 (7) ◽  
pp. 1161-1166 ◽  
Author(s):  
A. Muraglia ◽  
R. Cancedda ◽  
R. Quarto
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Mesenchymal Progenitors

Bone marrow stromal cells can give rise to several mesenchymal lineages. The existence of a common stem/progenitor cell, the mesenchymal stem cell, has been proposed, but which developmental stages follow this mesenchymal multipotent progenitor is not known. Based on experimental evidence, a model of mesenchymal stem cell differentiation has been proposed in which individual lineages branch directly from the same progenitor. We have verified this model by using clonal cultures of bone marrow derived stromal fibroblasts. We have analyzed the ability of 185 non-immortalized human bone marrow stromal cell clones to differentiate into the three main lineages: osteo-, chondro- and adipogenic. All clones but one differentiated into the osteogenic lineage. About one third of the clones differentiated into all three lineages analyzed. Most clones (60-80%) displayed an osteo-chondrogenic potential. We have never observed clones with a differentiation potential limited to the osteo-adipo- or to the chondro-adipogenic phenotype, nor pure chondrogenic and adipogenic clones. How long the differentiation potential of a number of clones was maintained was assessed throughout their life span. Clones progressively lost their adipogenic and chondrogenic differentiation potential at increasing cell doublings. Our data suggest a possible model of predetermined bone marrow stromal cells differentiation where the tripotent cells can be considered as early mesenchymal progenitors that display a sequential loss of lineage potentials, generating osteochondrogenic progenitors which, in turn, give rise to osteogenic precursors.

Therapeutic Impact of Human Bone Marrow Stromal Cells Expanded by Animal Serum–Free Medium for Cerebral Infarct in Rats

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1733-1742 ◽  
Author(s):  
Taku Sugiyama ◽  
Satoshi Kuroda ◽  
Yukari Takeda ◽  
Mitsufumi Nishio ◽  
Masaki Ito ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Therapeutic Impact
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