scholarly journals Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA

2020 ◽  
Vol 22 (7) ◽  
Author(s):  
Agnieszka Sałamaszyńska‐Guz ◽  
Izabela Serafińska ◽  
Paweł Bącal ◽  
Stephen Douthwaite
Keyword(s):  
Campylobacter Jejuni ◽  
Methylation Pattern ◽  
Its Rrna ◽  
Virulence Properties

scholarly journals Effect of Noradrenaline on the Virulence Properties ofCampylobacterSpecies

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sree V. Aroori ◽  
Tristan A. Cogan ◽  
Tom J. Humphrey
Keyword(s):  
Growth Rate ◽  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Immune Status ◽  
Acute Stress ◽  
Host Immunity ◽  
Stress Hormone ◽  
E Coli ◽  
Susceptibility To Infection ◽  
Virulence Properties

Campylobacterspecies cause a spectrum of illnesses in humans. The type of illness and the outcome is dependent on the virulence of the infecting pathogen strain and host immune status. Acute stress can seriously compromise host immunity and increase susceptibility to infection. Noradrenaline (NA) is a stress hormone. Several studies have shown that it stimulated growth and increased the pathogenicity of organisms includingE. coliandCampylobacter jejuni. However, the effect of NA on otherCampylobacterspecies is unknown. We have examined the effect of NA on growth rate, motility, invasion of T84 epithelial cells, and colonisation of chickens by diverseCampylobacterspecies.Campylobactercultures grown with NA had reduced lag phases, increased growth rates, and higher final optical densities than controls. The motility ofCampylobacterwas also significantly increased in the presence of noradrenaline. Some of theCampylobacterstrains tested also showed increased invasion of T84 epithelial cells, greater breakdown of tight junctions, and an enhanced potential to colonise chickens. Our results show that noradrenaline-induced enhancement of virulence ofCampylobactercan influence the outcome of infection.

scholarly journals Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties

2010 ◽  
Vol 78 (6) ◽  
pp. 2812-2822 ◽  
Author(s):  
Abofu Alemka ◽  
Marguerite Clyne ◽  
Fergus Shanahan ◽  
Thomas Tompkins ◽  
Nicolae Corcionivoschi ◽  
...  
Keyword(s):  
Cell Line ◽  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Early Time ◽  
Lactobacillus Helveticus ◽  
Mucus Layer ◽  
Junction Protein ◽  
Probiotic Treatment ◽  
Virulence Properties

ABSTRACT The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonized and reproduced in E12 mucus. Adhesion to and internalization of C. jejuni were enhanced in E12 cells harboring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalized with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains Lactobacillus rhamnosus R0011, Lactobacillus helveticus R0052, Lactobacillus salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (Lacidofil), and a cocktail consisting of L. rhamnosus, L. helveticus, and L. salivarius (RhHeSa) colonized E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with and internalization into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonization of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.

scholarly journals Virulence properties of Campylobacter jejuni isolates of poultry and human origin

2007 ◽  
Vol 56 (10) ◽  
pp. 1284-1289 ◽  
Author(s):  
Kim Van Deun ◽  
Freddy Haesebrouck ◽  
Marc Heyndrickx ◽  
Herman Favoreel ◽  
Jeroen Dewulf ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Sodium Deoxycholate ◽  
Salt Resistance ◽  
Poultry Meat ◽  
Human Origin ◽  
T84 Cells ◽  
Food Borne ◽  
High Prevalence ◽  
Two Populations ◽  
Virulence Properties

Campylobacter jejuni is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of C. jejuni in poultry, poultry meat is considered a major source of C. jejuni infections for humans. However, it is not known whether all poultry-associated C. jejuni strains are capable of causing disease in humans. Four different virulence properties of C. jejuni strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (P=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 μg sodium deoxycholate ml−1 was significantly more pronounced (P=0.006) among human isolates than poultry isolates, although all strains possessed the cmeABC operon. These data suggest that all four virulence properties are widespread among C. jejuni isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.

scholarly journals Comparison of Campylobacter Isolates from Poultry and Humans: Association between In Vitro Virulence Properties, Biotypes, and Pulsed-Field Gel Electrophoresis Clusters

2003 ◽  
Vol 69 (10) ◽  
pp. 6316-6320 ◽  
Author(s):  
Éric Nadeau ◽  
Serge Messier ◽  
Sylvain Quessy
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Vero Cells ◽  
Pulsed Field Gel Electrophoresis ◽  
Cell Cytotoxicity ◽  
Cho Cell ◽  
Pulsed Field ◽  
Virulence Properties ◽  
Invasiveness Potential

ABSTRACT The in vitro virulence properties of 197 temporally and geographically related Campylobacter isolates from chicken broilers and humans were compared. Comparisons of the virulence properties associated with genotypes and biotypes were made. All isolates adhered to, and 63% invaded, INT-407 cells, whereas 13% were cytotoxic for CHO cells. CHO cell-cytotoxic extracts were also cytotoxic for INT-407 cells, but the sensitivity for Vero cells was variable. The proportion of isolates demonstrating a high invasiveness potential (>1,000 CFU ml−1) or Vero cell cytotoxicity was significantly higher for human than for poultry isolates. Invasiveness was associated with Campylobacter jejuni isolates of biotypes 1 and 2, whereas CHO and INT-407 cell cytotoxicity was associated with C. jejuni isolates of biotypes 3 and 4. Cytotoxic isolates were also clustered according to pulsed-field gel electrophoresis profiles.

Impact of erythromycin resistance on the virulence properties and fitness of Campylobacter jejuni

2011 ◽  
Vol 50 (6) ◽  
pp. 336-342 ◽  
Author(s):  
Yassir A. Almofti ◽  
Menghong Dai ◽  
Yawei Sun ◽  
Hao Haihong ◽  
Zonghui Yuan
Keyword(s):  
Campylobacter Jejuni ◽  
Erythromycin Resistance ◽  
Virulence Properties

Ultrastructure of periplasmic bodies in gram-negative bacteria

1990 ◽  
Vol 48 (3) ◽  
pp. 640-641
Author(s):  
Xie Nianming ◽  
Ding Shaoqing ◽  
Wang Luping ◽  
Yuan Zenglin ◽  
Zhan Guolai ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Campylobacter Jejuni ◽  
Proteus Mirabilis ◽  
Shigella Flexneri ◽  
Morphological Description ◽  
Gram Negative Bacteria ◽  
Periplasmic Space ◽  
Clostridium Tetani ◽  
Gram Negative ◽  
Brucella Canis

Perhaps the data about periplasmic enzymes are obtained through biochemical methods but lack of morphological description. We have proved the existence of periplasmic bodies by electron microscope and described their ultrastructures. We hope this report may draw the attention of biochemists and mrophologists to collaborate on researches in periplasmic enzymes or periplasmic bodies with each other.One or more independent bodies may be seen in the periplasmic space between outer and inner membranes of Gram-negative bacteria, which we called periplasmic bodies. The periplasmic bodies have been found in seven species of bacteria at least, including the Pseudomonas aeroginosa. Shigella flexneri, Echerichia coli. Yersinia pestis, Campylobacter jejuni, Proteus mirabilis, Clostridium tetani. Vibrio cholerae and Brucella canis.

Investigations into the antibacterial activities of herbal medicines against Helicobacter pylori and Campylobacter jejuni

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
C Cwikla ◽  
K Schmidt ◽  
A Matthias ◽  
KM Bone ◽  
RP Lehmann ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Campylobacter Jejuni ◽  
Herbal Medicines ◽  
Antibacterial Activities
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