Impact of erythromycin resistance on the virulence properties and fitness of Campylobacter jejuni

2011 ◽  
Vol 50 (6) ◽  
pp. 336-342 ◽  
Author(s):  
Yassir A. Almofti ◽  
Menghong Dai ◽  
Yawei Sun ◽  
Hao Haihong ◽  
Zonghui Yuan
Keyword(s):  
Campylobacter Jejuni ◽  
Erythromycin Resistance ◽  
Virulence Properties

scholarly journals Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA

2020 ◽  
Vol 22 (7) ◽  
Author(s):  
Agnieszka Sałamaszyńska‐Guz ◽  
Izabela Serafińska ◽  
Paweł Bącal ◽  
Stephen Douthwaite
Keyword(s):  
Campylobacter Jejuni ◽  
Methylation Pattern ◽  
Its Rrna ◽  
Virulence Properties

scholarly journals Effect of Noradrenaline on the Virulence Properties ofCampylobacterSpecies

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sree V. Aroori ◽  
Tristan A. Cogan ◽  
Tom J. Humphrey
Keyword(s):  
Growth Rate ◽  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Immune Status ◽  
Acute Stress ◽  
Host Immunity ◽  
Stress Hormone ◽  
E Coli ◽  
Susceptibility To Infection ◽  
Virulence Properties

Campylobacterspecies cause a spectrum of illnesses in humans. The type of illness and the outcome is dependent on the virulence of the infecting pathogen strain and host immune status. Acute stress can seriously compromise host immunity and increase susceptibility to infection. Noradrenaline (NA) is a stress hormone. Several studies have shown that it stimulated growth and increased the pathogenicity of organisms includingE. coliandCampylobacter jejuni. However, the effect of NA on otherCampylobacterspecies is unknown. We have examined the effect of NA on growth rate, motility, invasion of T84 epithelial cells, and colonisation of chickens by diverseCampylobacterspecies.Campylobactercultures grown with NA had reduced lag phases, increased growth rates, and higher final optical densities than controls. The motility ofCampylobacterwas also significantly increased in the presence of noradrenaline. Some of theCampylobacterstrains tested also showed increased invasion of T84 epithelial cells, greater breakdown of tight junctions, and an enhanced potential to colonise chickens. Our results show that noradrenaline-induced enhancement of virulence ofCampylobactercan influence the outcome of infection.

scholarly journals Erratum to: Correlations between 23S rRNA genes and erythromycin resistance in Campylobacter jejuni

2012 ◽  
Vol 62 (4) ◽  
pp. 1841-1841
Author(s):  
Takuya Nakajima ◽  
Akihiro Tazumi ◽  
Shigeyuki Nakanishi ◽  
Jiru Xu ◽  
Lei Han ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Rrna Genes ◽  
23S Rrna ◽  
Erythromycin Resistance ◽  
23S Rrna Genes

scholarly journals Inaccuracy of routine susceptibility tests for detection of erythromycin resistance of Campylobacter jejuni and Campylobacter coli

2010 ◽  
Vol 16 (1) ◽  
pp. 51-56 ◽  
Author(s):  
M.T. van der Beek ◽  
E.C.J. Claas ◽  
D.J. Mevius ◽  
W. van Pelt ◽  
J.A. Wagenaar ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Erythromycin Resistance ◽  
Susceptibility Tests

scholarly journals Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties

2010 ◽  
Vol 78 (6) ◽  
pp. 2812-2822 ◽  
Author(s):  
Abofu Alemka ◽  
Marguerite Clyne ◽  
Fergus Shanahan ◽  
Thomas Tompkins ◽  
Nicolae Corcionivoschi ◽  
...  
Keyword(s):  
Cell Line ◽  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Early Time ◽  
Lactobacillus Helveticus ◽  
Mucus Layer ◽  
Junction Protein ◽  
Probiotic Treatment ◽  
Virulence Properties

ABSTRACT The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonized and reproduced in E12 mucus. Adhesion to and internalization of C. jejuni were enhanced in E12 cells harboring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalized with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains Lactobacillus rhamnosus R0011, Lactobacillus helveticus R0052, Lactobacillus salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (Lacidofil), and a cocktail consisting of L. rhamnosus, L. helveticus, and L. salivarius (RhHeSa) colonized E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with and internalization into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonization of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.

Correlations between 23S rRNA genes and erythromycin resistance in Campylobacter jejuni

2012 ◽  
Vol 62 (4) ◽  
pp. 1495-1500 ◽  
Author(s):  
Takuya Nakajima ◽  
Akihiro Tazumi ◽  
Shigeyuki Nakanishi ◽  
Jiru Xu ◽  
Lei Han ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Rrna Genes ◽  
23S Rrna ◽  
Erythromycin Resistance ◽  
23S Rrna Genes

scholarly journals Virulence properties of Campylobacter jejuni isolates of poultry and human origin

2007 ◽  
Vol 56 (10) ◽  
pp. 1284-1289 ◽  
Author(s):  
Kim Van Deun ◽  
Freddy Haesebrouck ◽  
Marc Heyndrickx ◽  
Herman Favoreel ◽  
Jeroen Dewulf ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Sodium Deoxycholate ◽  
Salt Resistance ◽  
Poultry Meat ◽  
Human Origin ◽  
T84 Cells ◽  
Food Borne ◽  
High Prevalence ◽  
Two Populations ◽  
Virulence Properties

Campylobacter jejuni is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of C. jejuni in poultry, poultry meat is considered a major source of C. jejuni infections for humans. However, it is not known whether all poultry-associated C. jejuni strains are capable of causing disease in humans. Four different virulence properties of C. jejuni strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (P=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 μg sodium deoxycholate ml−1 was significantly more pronounced (P=0.006) among human isolates than poultry isolates, although all strains possessed the cmeABC operon. These data suggest that all four virulence properties are widespread among C. jejuni isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.

scholarly journals Antimicrobial Susceptibilities of Multidrug-Resistant Campylobacter jejuni and C. coli Strains: In Vitro Activities of 20 Antimicrobial Agents

2009 ◽  
Vol 54 (3) ◽  
pp. 1232-1236 ◽  
Author(s):  
Mirva Lehtopolku ◽  
Ulla-Maija Nakari ◽  
Pirkko Kotilainen ◽  
Pentti Huovinen ◽  
Anja Siitonen ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Dilution Method ◽  
Erythromycin Resistance ◽  
Disk Diffusion Test ◽  
Resistant Strains

ABSTRACT There is a paucity of information regarding antimicrobial agents that are suitable to treat severe infections caused by multidrug-resistant Campylobacter spp. Our aim was to identify agents that are potentially effective against multiresistant Campylobacter strains. The in vitro activities of 20 antimicrobial agents against 238 Campylobacter strains were analyzed by determining MICs by the agar plate dilution method or the Etest. These strains were selected from 1,808 Campylobacter isolates collected from Finnish patients between 2003 and 2005 and screened for macrolide susceptibility by using the disk diffusion test. The 238 strains consisted of 183 strains with erythromycin inhibition zone diameters of ≤23 mm and 55 strains with inhibition zone diameters of >23 mm. Of the 238 Campylobacter strains, 19 were resistant to erythromycin by MIC determinations (MIC ≥ 16 μg/ml). Given that the resistant strains were identified among the collection of 1,808 isolates, the frequency of erythromycin resistance was 1.1%. All erythromycin-resistant strains were multidrug resistant, with 18 (94.7%) of them being resistant to ciprofloxacin (MIC ≥ 4 μg/ml). The percentages of resistance to tetracycline and amoxicillin-clavulanic acid (co-amoxiclav) were 73.7% and 31.6%, respectively. All macrolide-resistant strains were susceptible to imipenem, meropenem, and tigecycline. Ten (52.6%) multiresistant strains were identified as being Campylobacter jejuni strains, and 9 (47.4%) were identified as being C. coli strains. These data demonstrate that the incidence of macrolide resistance was low but that the macrolide-resistant Campylobacter strains were uniformly multidrug resistant. In addition to the carbapenems, tigecycline was also highly effective against these multidrug-resistant Campylobacter strains in vitro. Its efficacy for the treatment of human campylobacteriosis should be evaluated in clinical trials.

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