scholarly journals Effects of In Vivo Gene Transfer of Fibroblast Growth Factor-2 on Cardiac Function and Collateral Vessel Formation in the Microembolized Rabbit Heart

2001 ◽  
Vol 65 (3) ◽  
pp. 226-231 ◽  
Author(s):  
Mitsuo Iwatate ◽  
Toshiro Miura ◽  
Yasuhiro Ikeda ◽  
Shuji Kawamura ◽  
Yuka Dairaku ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Cardiac Function ◽  
Fibroblast Growth Factor ◽  
Rabbit Heart ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Collateral Vessel ◽  
In Vivo Gene Transfer

scholarly journals Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture

2021 ◽  
Author(s):  
Eishin Yaoita ◽  
Masaaki Nameta ◽  
Yutaka Yoshida ◽  
Hidehiko Fujinaka
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Quiescent State ◽  
Fibroblast Growth ◽  
Gene Expressions ◽  
Dynamic Changes ◽  
Ki 67

AbstractFibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.

In vivo electroporation enhances plasmid-based gene transfer of basic fibroblast growth factor for the treatment of ischemic limb

2004 ◽  
Vol 120 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Seiji Nishikage ◽  
Hiroyuki Koyama ◽  
Tetsuro Miyata ◽  
Shigeyuki Ishii ◽  
Hirohumi Hamada ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
In Vivo Electroporation ◽  
Ischemic Limb

scholarly journals Comparative Study In Vivo and In Vitro of Uniformly 14C-Labelled and 125I-Labelled Recombinant Fibroblast Growth Factor 2

1997 ◽  
Vol 249 (2) ◽  
pp. 473-480 ◽  
Author(s):  
Sylvie Colin ◽  
Frederic Mascarelli ◽  
Jean-Claude Jeanny ◽  
Raymond Vienet ◽  
Gerard Bouche ◽  
...  
Keyword(s):  
Growth Factor ◽  
Comparative Study ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth
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