Influence of different instrumentation modalities on the surface characteristics and biofilm formation on dental implant neck,in vitro

2016 ◽  
Vol 28 (4) ◽  
pp. 483-490 ◽  
Author(s):  
Kristina Emily Schmidt ◽  
Thorsten Mathias Auschill ◽  
Christian Heumann ◽  
Roland Frankenberger ◽  
Sigrun Eick ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Surface Characteristics

scholarly journals Biofilm formation on dental implant surfaces ‐ in vitro dynamic model

2019 ◽  
Vol 30 (S19) ◽  
pp. 8-8
Author(s):  
Honorato Ribeiro‐Vidal ◽  
Maria Del Carmen Sanchez ◽  
Elena Figuero ◽  
David Herrera ◽  
Mariano Sanz
Keyword(s):  
Dynamic Model ◽  
Dental Implant ◽  
Biofilm Formation

An in vitro biofilm model associated to dental implants: Structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces

2014 ◽  
Vol 30 (10) ◽  
pp. 1161-1171 ◽  
Author(s):  
M.C. Sánchez ◽  
A. Llama-Palacios ◽  
E. Fernández ◽  
E. Figuero ◽  
M.J. Marín ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dental Implants ◽  
Dental Implant ◽  
Biofilm Formation ◽  
Biofilm Model

scholarly journals Porphyromonas Gingivalis Biofilm Formation on an Implant Surface Treated With Nanoselenium

2020 ◽  
Author(s):  
Mohamed Assadawy ◽  
Eman Helmy
Keyword(s):  
Dental Implant ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Practical Implication ◽  
Antibacterial Properties ◽  
Implant Surface ◽  
Sem Images ◽  
Dental Implant Surface ◽  
Implant Coatings

Abstract Background: Biofilm formation on implants is the primary factor for implant loss. Porphyromonas gingivalis is a highly virulent pathogen that contributes to the development of periodontal disease and implant failure.Objectives: The goals of this study are to investigate the formation of P. gingivalis biofilms on nanoselenium-coated implants in vitro and the potential use of nanoselenium for peri-implantitis treatment.Materials and methods: Porphyromonas gingivalis ATCC 33277 was cultured to obtain an in vitro mature biofilm on the surface of the Hexacone implant system. The fixture was added into an Eppendorf tube and placed in a sterile air laminar flow cabinet. An automatic machine learning utility was used to calculate the biofilm size on the implant surface from SEM images, and the Trainable Weka Segmentation plugin in Fiji software was employed.Results The SeNPs affected the P. gingivalis biofilm (the effect size was 80.17%), and the difference was highly significant (p 0.000).Conclusion: The use of SeNPs as dental implant coatings presented promising anti-P. gingivalis biofilm activity.Clinical relevance:: The development of a dental implant surface treatment with efficient antibacterial properties, especially against the most virulent pathogens, has not yet been established.Principal findings: Nanoselenium particles as an implant surface coating prevented Porphyromonas gingivalis biofilm formation to a striking extent.Practical implication: Nanoparticles could provide a novel state-of-the-art therapeutic approach for Porphyromonas gingivalis (P. gingivalis biofilm on dental implants)

Does Finishing and Polishing of Restorative Materials Affect Bacterial Adhesion and Biofilm Formation? A Systematic Review

2018 ◽  
Vol 43 (1) ◽  
pp. E37-E52 ◽  
Author(s):  
DAM Dutra ◽  
GKR Pereira ◽  
KZ Kantorski ◽  
LF Valandro ◽  
FB Zanatta
Keyword(s):  
Systematic Review ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Surface Characteristics ◽  
Periodontal Inflammation ◽  
Restorative Materials ◽  
The Impact

SUMMARY Biofilm (bacterial plaque) accumulation on the surface of restorative materials favors the occurrence of secondary caries and periodontal inflammation. Surface characteristics of restorations can be modified by finishing and/or polishing procedures and may affect bacterial adhesion. The aim of this systematic review was to characterize how finishing and polishing methods affect the surface properties of different restorative materials with regard to bacterial adhesion and biofilm formation. Searches were carried out in MEDLINE-PubMed, EMBASE, Cochrane-CENTRAL, and LILACS databases. From 2882 potential articles found in the initial searches, only 18 met the eligible criteria and were included in this review (12 with in vitro design, four with in situ design, and two clinical trials). However, they presented high heterogeneity regarding materials considered and methodology for evaluating the desired outcome. Risk bias analysis showed that only two studies presented low risk (whereas 11 showed high and five showed medium risk). Thus, only descriptive analyses considering study design, materials, intervention (finishing/polishing), surface characteristics (roughness and surface free energy), and protocol for biofilm formation (bacterial adhesion) could be performed. Some conclusions could be drawn: the impact of roughness on bacterial adhesion seems to be related not to a roughness threshold (as previously believed) but rather to a range, the range of surface roughness among different polishing methods is wide and material dependent, finishing invariably creates a rougher surface and should always be followed by a polishing method, each dental material requires its own treatment modality to obtain and maintain as smooth a surface as possible, and in vitro designs do not seem to be powerful tools to draw relevant conclusions, so in vivo and in situ designs become strongly recommended.

scholarly journals Influence of Curing Time on the Microbiological Behavior of Bulk-Fill Nanohybrid Resin Composites

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2948
Author(s):  
Andrei C. Ionescu ◽  
Allegra Comba ◽  
Eugenio Brambilla ◽  
Nicoleta Ilie ◽  
Lorenzo Breschi ◽  
...  
Keyword(s):  
Elemental Composition ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Surface Characteristics ◽  
Matrix Composition ◽  
Resin Matrix ◽  
Curing Time ◽  
Lower Adherence ◽  
Fill Materials

This in vitro study aimed to evaluate the influence of curing time on surface characteristics and microbiological behavior of three bulk-fill resin-based composites (RBCs). Materials were light-cured for either 10 s or 80 s, then finished using a standard clinical procedure. They were characterized by surface morphology (SEM), surface elemental composition (EDS), surface roughness (SR), and surface free energy (SFE). Microbiological behavior was assessed as S. mutans adherence (2 h) and biofilm formation (24 h) using a continuous-flow bioreactor. Statistical analysis included a two-way ANOVA and Tukey’s test (p < 0.05). Materials differed substantially as filler shape, dimension, elemental composition and resin matrix composition. Significant differences between materials were found for SR, SFE, and microbiological behavior. Such differences were less pronounced or disappeared after prolonged photocuring. The latter yielded significantly lower adherence and biofilm formation on all tested materials, similar to conventional RBCs. Improved photoinitiators and UDMA-based resin matrix composition may explain these results. No correlation between surface characteristics and microbiological behavior can explain the similar microbiological behavior of bulk-fill materials after prolonged photocuring. This different performance of bulk-fill materials compared with conventional RBCs, where surface characteristics, especially surface chemistry, influence microbiological behavior, may have important implications for secondary caries occurrence and restoration longevity.

scholarly journals Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

2018 ◽  
Vol 34 (2) ◽  
pp. 272-281 ◽  
Author(s):  
Aifang Han ◽  
James K.H. Tsoi ◽  
Jukka P. Matinlinna ◽  
Yu Zhang ◽  
Zhuofan Chen
Keyword(s):  
Biofilm Formation ◽  
Surface Characteristics

Surface characteristics and in vitro biofilm formation on glass ionomer and composite resin

Biomaterials ◽  
2001 ◽  
Vol 22 (5) ◽  
pp. 481-487 ◽  
Author(s):  
A. Carlén ◽  
K. Nikdel ◽  
A. Wennerberg ◽  
K. Holmberg ◽  
J. Olsson
Keyword(s):  
Biofilm Formation ◽  
Composite Resin ◽  
Surface Characteristics ◽  
Glass Ionomer

scholarly journals In vitro Interactions between Streptococcus intermedius and Streptococcus salivarius K12 on a Titanium Cylindrical Surface

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1069
Author(s):  
Carla Vacca ◽  
Maria Paola Contu ◽  
Cecilia Rossi ◽  
Maria Laura Ferrando ◽  
Cornelio Blus ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Expression Patterns ◽  
Formation Rate ◽  
Bacterial Count ◽  
Probiotic Bacterium ◽  
Streptococcus Salivarius ◽  
Streptococcus Intermedius ◽  
Commercial Probiotics

Peri-implantitis is a steadily rising disease and is caused by oral bacterial pathogens able to form biofilm on implant surfaces and peri-implant tissues, making antibiotics treatment less effective. The use of commercial probiotics against oral pathogens could serve as an alternative to prevent biofilm formation. Streptococcus intermedius is one of the early colonizers of biofilm formation in dental implants. The aim of this study was to model the interaction between S. intermedius and Streptococcus salivarius strain K12, a probiotic bacterium producing bacteriocins. S. intermedius was co-cultured with S. salivarius K12 in an in vitro model simulating the biofilm formation in a dental implant composed by a titanium cylinder system. Biofilm formation rate was assessed by Real-Time PCR quantification of bacterial count and expression levels of luxS gene, used in response to cell density in the biofilm. Biofilm formation, bacteriocin production, luxS expression patterns were found to be already expressed within the first 12 h. More importantly, S. salivarius K12 was able to counter the biofilm formation in a titanium cylinder under the tested condition. In conclusion, our dental implant model may be useful for exploring probiotic-pathogen interaction to find an alternative to antibiotics for peri-implantitis treatment.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

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