scholarly journals Biofilm formation on dental implant surfaces ‐ in vitro dynamic model

2019 ◽  
Vol 30 (S19) ◽  
pp. 8-8
Author(s):  
Honorato Ribeiro‐Vidal ◽  
Maria Del Carmen Sanchez ◽  
Elena Figuero ◽  
David Herrera ◽  
Mariano Sanz
Keyword(s):  
Dynamic Model ◽  
Dental Implant ◽  
Biofilm Formation

Influence of different instrumentation modalities on the surface characteristics and biofilm formation on dental implant neck,in vitro

2016 ◽  
Vol 28 (4) ◽  
pp. 483-490 ◽  
Author(s):  
Kristina Emily Schmidt ◽  
Thorsten Mathias Auschill ◽  
Christian Heumann ◽  
Roland Frankenberger ◽  
Sigrun Eick ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Surface Characteristics

An in vitro biofilm model associated to dental implants: Structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces

2014 ◽  
Vol 30 (10) ◽  
pp. 1161-1171 ◽  
Author(s):  
M.C. Sánchez ◽  
A. Llama-Palacios ◽  
E. Fernández ◽  
E. Figuero ◽  
M.J. Marín ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dental Implants ◽  
Dental Implant ◽  
Biofilm Formation ◽  
Biofilm Model

scholarly journals Porphyromonas Gingivalis Biofilm Formation on an Implant Surface Treated With Nanoselenium

2020 ◽  
Author(s):  
Mohamed Assadawy ◽  
Eman Helmy
Keyword(s):  
Dental Implant ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Practical Implication ◽  
Antibacterial Properties ◽  
Implant Surface ◽  
Sem Images ◽  
Dental Implant Surface ◽  
Implant Coatings

Abstract Background: Biofilm formation on implants is the primary factor for implant loss. Porphyromonas gingivalis is a highly virulent pathogen that contributes to the development of periodontal disease and implant failure.Objectives: The goals of this study are to investigate the formation of P. gingivalis biofilms on nanoselenium-coated implants in vitro and the potential use of nanoselenium for peri-implantitis treatment.Materials and methods: Porphyromonas gingivalis ATCC 33277 was cultured to obtain an in vitro mature biofilm on the surface of the Hexacone implant system. The fixture was added into an Eppendorf tube and placed in a sterile air laminar flow cabinet. An automatic machine learning utility was used to calculate the biofilm size on the implant surface from SEM images, and the Trainable Weka Segmentation plugin in Fiji software was employed.Results The SeNPs affected the P. gingivalis biofilm (the effect size was 80.17%), and the difference was highly significant (p 0.000).Conclusion: The use of SeNPs as dental implant coatings presented promising anti-P. gingivalis biofilm activity.Clinical relevance:: The development of a dental implant surface treatment with efficient antibacterial properties, especially against the most virulent pathogens, has not yet been established.Principal findings: Nanoselenium particles as an implant surface coating prevented Porphyromonas gingivalis biofilm formation to a striking extent.Practical implication: Nanoparticles could provide a novel state-of-the-art therapeutic approach for Porphyromonas gingivalis (P. gingivalis biofilm on dental implants)

scholarly journals In vitro Interactions between Streptococcus intermedius and Streptococcus salivarius K12 on a Titanium Cylindrical Surface

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1069
Author(s):  
Carla Vacca ◽  
Maria Paola Contu ◽  
Cecilia Rossi ◽  
Maria Laura Ferrando ◽  
Cornelio Blus ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Expression Patterns ◽  
Formation Rate ◽  
Bacterial Count ◽  
Probiotic Bacterium ◽  
Streptococcus Salivarius ◽  
Streptococcus Intermedius ◽  
Commercial Probiotics

Peri-implantitis is a steadily rising disease and is caused by oral bacterial pathogens able to form biofilm on implant surfaces and peri-implant tissues, making antibiotics treatment less effective. The use of commercial probiotics against oral pathogens could serve as an alternative to prevent biofilm formation. Streptococcus intermedius is one of the early colonizers of biofilm formation in dental implants. The aim of this study was to model the interaction between S. intermedius and Streptococcus salivarius strain K12, a probiotic bacterium producing bacteriocins. S. intermedius was co-cultured with S. salivarius K12 in an in vitro model simulating the biofilm formation in a dental implant composed by a titanium cylinder system. Biofilm formation rate was assessed by Real-Time PCR quantification of bacterial count and expression levels of luxS gene, used in response to cell density in the biofilm. Biofilm formation, bacteriocin production, luxS expression patterns were found to be already expressed within the first 12 h. More importantly, S. salivarius K12 was able to counter the biofilm formation in a titanium cylinder under the tested condition. In conclusion, our dental implant model may be useful for exploring probiotic-pathogen interaction to find an alternative to antibiotics for peri-implantitis treatment.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

scholarly journals In VitroAnalyses of the Effects of Heparin and Parabens on Candida albicans Biofilms and Planktonic Cells

2011 ◽  
Vol 56 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Marisa H. Miceli ◽  
Stella M. Bernardo ◽  
T. S. Neil Ku ◽  
Carla Walraven ◽  
Samuel A. Lee
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
High Dose ◽  
Dependent Manner ◽  
Planktonic Cells ◽  
Content Type ◽  
Heparin Sodium ◽  
The Individual

ABSTRACTInfections and thromboses are the most common complications associated with central venous catheters. Suggested strategies for prevention and management of these complications include the use of heparin-coated catheters, heparin locks, and antimicrobial lock therapy. However, the effects of heparin onCandida albicansbiofilms and planktonic cells have not been previously studied. Therefore, we sought to determine thein vitroeffect of a heparin sodium preparation (HP) on biofilms and planktonic cells ofC. albicans. Because HP contains two preservatives, methyl paraben (MP) and propyl paraben (PP), these compounds and heparin sodium without preservatives (Pure-H) were also tested individually. The metabolic activity of the mature biofilm after treatment was assessed using XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and microscopy. Pure-H, MP, and PP caused up to 75, 85, and 60% reductions of metabolic activity of the mature preformedC. albicansbiofilms, respectively. Maximal efficacy against the mature biofilm was observed with HP (up to 90%) compared to the individual compounds (P< 0.0001). Pure-H, MP, and PP each inhibitedC. albicansbiofilm formation up to 90%. A complete inhibition of biofilm formation was observed with HP at 5,000 U/ml and higher. When tested against planktonic cells, each compound inhibited growth in a dose-dependent manner. These data indicated that HP, MP, PP, and Pure-H havein vitroantifungal activity againstC. albicansmature biofilms, formation of biofilms, and planktonic cells. Investigation of high-dose heparin-based strategies (e.g., heparin locks) in combination with traditional antifungal agents for the treatment and/or prevention ofC. albicansbiofilms is warranted.

scholarly journals The Impact of Dental Implant Surface Modifications on Osseointegration and Biofilm Formation

2021 ◽  
Vol 10 (8) ◽  
pp. 1641
Author(s):  
Stefanie Kligman ◽  
Zhi Ren ◽  
Chun-Hsi Chung ◽  
Michael Angelo Perillo ◽  
Yu-Cheng Chang ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Surface Modifications ◽  
Implant Surface ◽  
Oral Rehabilitation ◽  
Surface Design ◽  
Polyether Ether Ketone ◽  
Dental Implant Surface ◽  
The Impact

Implant surface design has evolved to meet oral rehabilitation challenges in both healthy and compromised bone. For example, to conquer the most common dental implant-related complications, peri-implantitis, and subsequent implant loss, implant surfaces have been modified to introduce desired properties to a dental implant and thus increase the implant success rate and expand their indications. Until now, a diversity of implant surface modifications, including different physical, chemical, and biological techniques, have been applied to a broad range of materials, such as titanium, zirconia, and polyether ether ketone, to achieve these goals. Ideal modifications enhance the interaction between the implant’s surface and its surrounding bone which will facilitate osseointegration while minimizing the bacterial colonization to reduce the risk of biofilm formation. This review article aims to comprehensively discuss currently available implant surface modifications commonly used in implantology in terms of their impact on osseointegration and biofilm formation, which is critical for clinicians to choose the most suitable materials to improve the success and survival of implantation.

Sign in / Sign up

Export Citation Format

Share Document