scholarly journals Lnc-NTF3-5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR-93-3p

2017 ◽  
Vol 20 (2) ◽  
pp. 110-121 ◽  
Author(s):  
Wei Peng ◽  
Shuang-Xi Zhu ◽  
Jin Wang ◽  
Li-Li Chen ◽  
Jun-Quan Weng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Membrane

scholarly journals Hsa_circRNA_33287 promotes the osteogenic differentiation of maxillary sinus membrane stem cells via miR-214-3p/Runx3

2019 ◽  
Vol 109 ◽  
pp. 1709-1717 ◽  
Author(s):  
Wei Peng ◽  
Shuangxi Zhu ◽  
Junlan Chen ◽  
Jin Wang ◽  
Qiong Rong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Membrane

miR-27b-3p Suppressed Osteogenic Differentiation of Maxillary Sinus Membrane Stem Cells by Targeting Sp7

2017 ◽  
Vol 26 (4) ◽  
pp. 492-499 ◽  
Author(s):  
Wei Peng ◽  
Shuangxi Zhu ◽  
Xiang Li ◽  
Junquan Weng ◽  
Songling Chen
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Membrane

scholarly journals Stiffness Regulates the Morphology, Adhesion, Proliferation, and Osteogenic Differentiation of Maxillary Schneiderian Sinus Membrane-Derived Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Yiping Liu ◽  
Jia Wang ◽  
Peisong Zhai ◽  
Sicong Ren ◽  
Zhanqi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Cell Fate ◽  
Osteogenic Potential ◽  
Future Research ◽  
Sinus Augmentation ◽  
Differentiation Potential ◽  
Maxillary Sinus Augmentation ◽  
Sinus Membrane

Recent studies, which aim to optimize maxillary sinus augmentation, have paid significant attention exploring osteogenic potential of maxillary Schneiderian sinus membrane-derived cells (MSSM-derived cells). However, it remains unclear that how MSSM-derived cells could respond to niche’s biomechanical properties. Herein, this study investigated the possible effects of substrate stiffness on rMSSM-derived stem cell fate. Initially, rMSSM-derived stem cells with multiple differentiation potential were successfully obtained. We then fabricated polyacrylamide substrates with varied stiffness ranging from 13 to 68 kPa to modulate the mechanical environment of rMSSM-derived stem cells. A larger cell spreading area and increased proliferation of rMSSM-derived stem cells were found on the stiffer substrates. Similarly, cells became more adhesive as their stiffness increased. Furthermore, the higher stiffness facilitated osteogenic differentiation of rMSSM-derived stem cells. Overall, our results indicated that increase in stiffness could mediate behaviors of rMSSM-derived stem cells, which may serve as a guide in future research to design novel biomaterials for maxillary sinus augmentation.

scholarly journals Promoting effect of rapamycin on osteogenic differentiation of maxillary sinus membrane stem cells

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11513
Author(s):  
Yanjun Lin ◽  
Min Zhang ◽  
Lin Zhou ◽  
Xuxi Chen ◽  
Jiang Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Promoting Effect ◽  
Alizarin Red ◽  
Real Time Quantitative Pcr ◽  
Alp Activity ◽  
Transmission Electron ◽  
Sinus Membrane

Background Stem cells located in the maxillary sinus membrane can differentiate into osteocytes. Our study aimed to evaluate the effect of rapamycin (RAPA) on the osteogenic differentiation of maxillary sinus membrane stem cells (MSMSCs). Methods Colony-forming unit assay, immunophenotype identification assay, and multi-differentiation assay confirmed characteristics of MSMSCs obtained from SD rats. Transmission electron microscopy (TEM) and flow cytometry (FCM) identified the initial autophagic level of MSMSCs induced by RAPA. Real-time quantitative PCR (qPCR) evaluated subsequent autophagic levels and osteogenic differentiation. Alkaline phosphatase (ALP) activity assay and alizarin red staining (ARS) evaluated subsequent osteogenic differentiation. We performed a histological examination to clarify in vivo osteogenesis with ectopic bone mass from BALB/c nude mice. Results MSMSCs possessed an active proliferation and multi-differentiation capacity, showing a phenotype of mesenchymal stem cells. The autophagic level increased with increasing RAPA (0, 10, 100, 1,000 nM) and decreased over time. ALP activity and calcium nodules forming in four RAPA-treated groups on three-time points (7, 14, 21 d) showed significant differences. Col1a1, Runx2, and Spp1 expressed most in 100 nM RAPA group on 7 and 14 d. Osteogenesis-related genes except for Ibsp expression between four groups tended to be consistent on 21 d. 100 nM and 10 nM RAPA-treated groups showed more bone formation in vivo. Conclusion RAPA can promote osteogenic differentiation of MSMSCs, indicating a possible relationship between osteogenic differentiation and autophagy.

Mesenchymal stem cells with osteogenic potential in human maxillary sinus membrane: an in vitro study

2016 ◽  
Vol 21 (5) ◽  
pp. 1599-1609 ◽  
Author(s):  
Antoine Berbéri ◽  
Fatima Al-Nemer ◽  
Eva Hamade ◽  
Ziad Noujeim ◽  
Bassam Badran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Maxillary Sinus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Sinus Membrane

scholarly journals Investigation of multipotent postnatal stem cells from human maxillary sinus membrane

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
JunBing Guo ◽  
JunQuan Weng ◽  
Qiong Rong ◽  
Xing Zhang ◽  
ShuangXi Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Maxillary Sinus ◽  
Sinus Membrane

scholarly journals Effects of Platelet-rich Fibrin on Osteogenic Differentiation of Schneiderian Membrane Derived Mesenchymal Stem Cells and Bone Formation in Maxillary Sinus

2021 ◽  
Author(s):  
Yanmin Zhou ◽  
Jia Wang ◽  
Yue Sun ◽  
Yiping Liu ◽  
Jize Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Floor Elevation ◽  
Schneiderian Membrane ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

Abstract BackgroundThe existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation.MethodsSchneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolate from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium (OM) was evaluated by alkaline phosphatase (ALP) staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery.ResultsThe cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF can stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which is achieved by up-regulating ERK 1/2 signaling pathway. PRF can accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. ConclusionsMSCs exist in Schneiderian membrane, and PRF stimulation can promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation can accelerate bone healing and increase the quantity and quality of new bone.

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