Rational design of peptide derivatives for inhibition of MyD88-mediated toll-like receptor signaling in human peripheral blood mononuclear cells and epithelial cells exposed toFrancisella tularensis

2017 ◽  
Vol 90 (6) ◽  
pp. 1190-1205 ◽  
Author(s):  
Daniel A. Ryan ◽  
Melissa Degardin ◽  
Shahabuddin Alam ◽  
Teri L. Kissner ◽  
Martha Hale ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Rational Design ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Peptide Derivatives

Regulatory Effects of High Glucose on the Expression of Toll-Like Receptor Gene and Cytokine Production in Human Peripheral-Blood Mononuclear Cells

2011 ◽  
Vol 179-180 ◽  
pp. 374-379 ◽  
Author(s):  
Jing Sheng Lan ◽  
Yu Kang Dong ◽  
Xing Ming Cai
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
High Glucose ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α ◽  
Blood Mononuclear Cells ◽  
Regulatory Effects

To investigate the regulatory effects of high glucose on the expression of Toll Like Receptor(TLR) Gene and the level of tumor necrosis factor(TNF)-α and interleukin(IL) -6 in human peripheral-blood mononuclear cells . Methods The alterations of TLR1～10 mRNA expression in human peripheral-blood mononuclear cells were quantitated using real-time quantitative-polymerasechain reaction. The level of TNF-α and IL-6 were measured by Enzyme-Linked Immuno Sorbent Assay . The anti-TLRmAb were used to block the mononuclear cells 30 min, high glucose was used to stimulate the cells. Rusults high glucose strongly up-regulated the expression of TLR3、5 mRNA but the expression of the other sub-TLRs weren’t changed .The concentrations of TNF-α、IL-6 were 86.40±8.46 and 874.66±92.84 in high glucose group, all significantly higher than that the control experiment (60.49±6.80 and 541.80±81.08 , all P<0.01), The anti-TLR3mAb and anti-TLR5mAb were used to block the mononuclear cells , The concentrations of TNF-α、IL-6 (72.41±8.52 and 700.59±84.88) were lower than that in the high glucose group, all P<0.05 . Conclusion high glucose may be endogenous ligand of TLRs and high glucose can regulate the release of inflammation cytokines from human peripheral-blood mononuclear cells through TLR signal way.

Differential expression of toll-like receptor signaling cascades in LPS-tolerant human peripheral blood mononuclear cells☆

Immunobiology ◽  
2011 ◽  
Vol 216 (3) ◽  
pp. 285-295 ◽  
Author(s):  
Marialice Erdelyi Mendes ◽  
Giovana Lotici Baggio-Zappia ◽  
Milena Karina Coló Brunialti ◽  
Maria da Luz Fernandes ◽  
Marjorie Marini Rapozo ◽  
...  
Keyword(s):  
Differential Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Signaling Cascades ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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