252-LB: Oxidative Stress Induces Toll-Like Receptor -2 and -4 in Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 252-LB
Author(s):  
SARDAR T. SINDHU ◽  
ASHRAF AL MADHOUN ◽  
NADEEM AKHTER ◽  
FAHD AL-MULLA ◽  
RASHEED AHMAD
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Metabolic Inflammation ◽  
Blood Mononuclear Cells ◽  
Toll Like Receptor 2

scholarly journals Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

IUBMB Life ◽  
2020 ◽  
Vol 72 (7) ◽  
pp. 1504-1514 ◽  
Author(s):  
Ali R. Bastin ◽  
Asie Sadeghi ◽  
Moslem Abolhassani ◽  
Amir H. Doustimotlagh ◽  
Abbas Mohammadi
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Enhancement of Chemokine mRNA Expression by Toll-Like Receptor 2 Stimulation in Human Peripheral Blood Mononuclear Cells of Patients with Atopic Dermatitis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Yangyang Yu ◽  
Dongxu Lin ◽  
Xiaoqiong Cai ◽  
Danni Cui ◽  
Ran Fang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mrna Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Healthy Controls ◽  
Toll Like Receptor ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Toll Like Receptor 2

Atopic dermatitis (AD) is a chronic inflammatory skin disease which is often associated with Staphylococcus aureus (S. aureus) colonization. S. aureus ingredients are potential ligands to activate the Toll-like receptor 2 (TLR2) and drive inflammatory cytokine or chemokine production. However, the role of TLR2-mediated chemokine expression in AD development has not been systematically investigated. In this study, we sought to determine the mode of TLR2-mediated chemokine expression in AD patients. Human peripheral blood mononuclear cells (PBMCs) were isolated from AD patients and healthy controls. Upon incubation with TLR2 ligands Pam3CSK4 and PGN, mRNA expression of chemokines, including CCL1, CCL5, CCL8, CCL13, CCL17, CCL18, CCL22, and CCL27, were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The results showed that basal mRNA expression of CCL17 in PBMCs from AD patients was upregulated compared with healthy controls, while those of CCL8 and CCL13 were downregulated. When stimulated with TLR2 ligands, the mRNA expression of CCL5, CCL8, CCL13, CCL18, and CCL22 in PBMCs from AD patients was significantly higher than those from healthy controls. The different basal chemokine mRNA expression profiles indicate the different immune status in patients with AD compared with healthy controls. Excessive chemokine mRNA expression induced by TLR2 activation is associated with the development of AD.

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