scholarly journals Droplet digital polymerase chain reaction assay and peptide nucleic acid-locked nucleic acid clamp method for RHOA mutation detection in angioimmunoblastic T-cell lymphoma

2018 ◽  
Vol 109 (5) ◽  
pp. 1682-1689 ◽  
Author(s):  
Sharna Tanzima Nuhat ◽  
Mamiko Sakata-Yanagimoto ◽  
Daisuke Komori ◽  
Keiichiro Hattori ◽  
Yasuhito Suehara ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Mutation Detection ◽  
Cell Lymphoma ◽  
Locked Nucleic Acid ◽  
Chain Reaction ◽  
Angioimmunoblastic T Cell Lymphoma ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

scholarly journals Inhibition of microRNA-21 via locked nucleic acid-anti-miR suppressed metastatic features of colorectal cancer cells through modulation of programmed cell death 4

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769226 ◽  
Author(s):  
Reza Nedaeinia ◽  
Mohammadreza Sharifi ◽  
Amir Avan ◽  
Mohammad Kazemi ◽  
Abdolreza Nabinejad ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Polymerase Chain Reaction ◽  
Cell Death ◽  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Chain Reaction ◽  
Programmed Cell Death 4 ◽  
Polymerase Chain ◽  
Novel Therapeutic

Colorectal cancer is among the most lethal of malignancies, due to its propensity to metastatic spread and multifactorial-chemoresistance. The latter property supports the need to identify novel therapeutic approaches for the treatment of colorectal cancer. MicroRNAs are endogenous non-coding small RNA molecules that function as post-transcriptional regulators of gene expression. Recently, programmed cell death 4 has been identified as a protein that increases during apoptosis. This gene is among the potential targets of miR-21 (OncomiR). Locked nucleic acid–modified oligonucleotides have recently emerged as a potential therapeutic option for targeting microRNAs. The aim of this study was to explore the functional role of locked nucleic acid-anti-miR-21 in the LS174T cell line in vitro and in vivo models. LS174T cells were treated with locked nucleic acid-anti-miR-21 for 24, 48, and 72 h in vitro. The expression of miR-21 and PDCD4 at messenger RNA (mRNA) level was evaluated by quantitative real-time polymerase chain reaction, while the protein level of PDCD4 was determined by Western blotting. Cell migratory behavior and the cluster-forming ability of cells were assessed before and after therapy. The disseminated tumor cells were assessed in the chick chorioallantoic membrane model by Alu quantitative polymerase chain reaction. Locked nucleic acid-anti-miR-21 was transfected successfully into the LS174T cells and inhibited the expression of miR-21. Locked nucleic acid-anti-miR-21 inhibited the migration and the number of cells forming clusters. Moreover, we found that locked nucleic acid-anti-miR-21 transfection was associated with a significant reduction in metastatic properties as assessed by the in ovo model. Our findings demonstrated the novel therapeutic potential of locked nucleic acid-anti-miR-21 in colon adenocarcinoma with high miR-21 expression.

TERTp Mutation Detection in Plasma by Droplet-Digital Polymerase Chain Reaction in Spinal Myxopapillary Ependymoma with Lung Metastases

2019 ◽  
Vol 130 ◽  
pp. 405-409 ◽  
Author(s):  
Anne Deniel ◽  
Florent Marguet ◽  
Ludivine Beaussire ◽  
Anne-Claire Tobenas-Dujardin ◽  
Christophe Peillon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mutation Detection ◽  
Lung Metastases ◽  
Myxopapillary Ependymoma ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

Investigation on Natural Diets of Larval Marine Animals Using Peptide Nucleic Acid-Directed Polymerase Chain Reaction Clamping

2010 ◽  
Vol 13 (2) ◽  
pp. 305-313 ◽  
Author(s):  
Seinen Chow ◽  
Sayaka Suzuki ◽  
Tadashi Matsunaga ◽  
Shane Lavery ◽  
Andrew Jeffs ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Chain Reaction ◽  
Marine Animals ◽  
Polymerase Chain
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