scholarly journals Novel 3‐D cell culture system for in vitro evaluation of anticancer drugs under anchorage‐independent conditions

2016 ◽  
Vol 107 (12) ◽  
pp. 1858-1866 ◽  
Author(s):  
Ayako Aihara ◽  
Natsuki Abe ◽  
Koichiro Saruhashi ◽  
Tatsuro Kanaki ◽  
Taito Nishino
Keyword(s):  
Cell Culture ◽  
Anticancer Drugs ◽  
Culture System ◽  
Cell Culture System ◽  
In Vitro Evaluation ◽  
D Cell

scholarly journals <i>In Vitro</i> Evaluation of Anticancer Drugs with Kinetic and Static Alternating Cell Culture System

2017 ◽  
Vol 08 (09) ◽  
pp. 845-859 ◽  
Author(s):  
Yongxin Zhang ◽  
Ying Wang ◽  
Zhenying Wang ◽  
Farhang Farhangfar ◽  
Monica Zimmerman
Keyword(s):  
Cell Culture ◽  
Anticancer Drugs ◽  
Culture System ◽  
Cell Culture System

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Effect of ascorbic acid and citric acid on bioavailability of iron from Tegillarca granosa via an in vitro digestion/Caco-2 cell culture system

2021 ◽  
Author(s):  
Bolun Sun ◽  
Beibei Tan ◽  
Sun Nan ◽  
Ping Huang ◽  
Jingxia Hong ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ascorbic Acid ◽  
Culture System ◽  
Iron Deficiency Anaemia ◽  
In Vitro Digestion ◽  
Cell Culture System ◽  
Iron Supplements ◽  
Deficiency Anaemia ◽  
Tegillarca Granosa

Iron deficiency anaemia (IDA) has been brought to worldwide attention. Developing safe and effective iron supplements is of great significance for IDA treatment. Tegillarca granosa (T. granosa), a traditional aquaculture...

Comparison between clinical response and in vitro drug sensitivity of primary human tumors in the adhesive tumor cell culture system.

1987 ◽  
Vol 5 (12) ◽  
pp. 1912-1921 ◽  
Author(s):  
J A Ajani ◽  
F L Baker ◽  
G Spitzer ◽  
A Kelly ◽  
W Brock ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Culture System ◽  
Distinct Advantage ◽  
Cell Culture System ◽  
True Value ◽  
Tumor Cell Culture ◽  
Prospective Trials ◽  
Tumor Types

The newly described adhesive tumor cell culture system (ATCCS) offers a distinct advantage over other assays in that it has a high plating efficiency requiring low cell inoculum, it affords workable assays in approximately 70% of specimens from the heterogenous tumor types, and it has the ability to assay up to nine drugs at four different concentrations. Clinical correlations based on the ATCCS were obtained in 65 patients undergoing 71 clinical trials. Patients with melanoma, lung cancer, and sarcoma dominated the group. The most active in vitro drug was correlated per clinical trial. Thirteen of 17 (76%) sensitive in vitro predictions and 51 of 54 (94%) resistant in vitro predictions were accurate. The assay in this study had a sensitivity of 81% and specificity of 93%. These preliminary results are encouraging and warrant prospective trials to establish the true value of this assay to patients.

SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

2005 ◽  
Vol 32 (6Part6) ◽  
pp. 1944-1944 ◽  
Author(s):  
R Bromley ◽  
L Oliver ◽  
R Harvie ◽  
R Davey
Keyword(s):  
Cell Culture ◽  
Biological Effect ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
A Cell ◽  
Vitro Model
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