scholarly journals Specialised endocytic proteins regulate diverse internalisation mechanisms and signalling outputs in physiology and cancer

2020 ◽  
Author(s):  
Giovanni Giangreco ◽  
Maria Grazia Malabarba ◽  
Sara Sigismund
Keyword(s):  
Endocytic Proteins

scholarly journals Differential Nucleocytoplasmic Trafficking between the Related Endocytic Proteins Eps15 and Eps15R

2002 ◽  
Vol 277 (11) ◽  
pp. 8941-8948 ◽  
Author(s):  
Viviane Poupon ◽  
Simona Polo ◽  
Manuela Vecchi ◽  
Gwendal Martin ◽  
Alice Dautry-Varsat ◽  
...  
Keyword(s):  
Nucleocytoplasmic Trafficking ◽  
Endocytic Proteins

scholarly journals Nucleocytoplasmic Shuttling of Endocytic Proteins

2001 ◽  
Vol 153 (7) ◽  
pp. 1511-1518 ◽  
Author(s):  
Manuela Vecchi ◽  
Simona Polo ◽  
Viviane Poupon ◽  
Jan-Willem van de Loo ◽  
Alexandre Benmerah ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Nuclear Export ◽  
Myeloid Leukemia ◽  
Nuclear Translocation ◽  
Initial Rate ◽  
Nucleocytoplasmic Shuttling ◽  
Cellular Processes ◽  
Transactivation Assay ◽  
Ordered Assembly ◽  
Endocytic Proteins

Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and α-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.

scholarly journals Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells

2019 ◽  
Vol 151 (12) ◽  
pp. 1386-1403 ◽  
Author(s):  
Dominique C. Stephens ◽  
Nicole Osunsanmi ◽  
Kem A. Sochacki ◽  
Tyrel W. Powell ◽  
Justin W. Taraska ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Secretory Vesicle ◽  
Effector Proteins ◽  
Cellular Transport ◽  
Secretory Vesicles ◽  
Rab Gtpases ◽  
Regulated Exocytosis ◽  
Endocytic Proteins

Altered regulation of exocytosis is an important mechanism controlling many diseases, including cancer. Defects in exocytosis have been implicated in many cancer cell types and are generally attributed to mutations in cellular transport, trafficking, and assembly of machinery necessary for exocytosis of secretory vesicle cargo. In these cancers, up-regulation of trafficking and secretion of matrix metalloproteinase-9 (MMP-9), a proteolytic enzyme, is responsible for degrading the extracellular matrix, a necessary step in tumor progression. Using TIRF microscopy, we identified proteins associated with secretory vesicles containing MMP-9 and imaged the local dynamics of these proteins at fusion sites during regulated exocytosis of MMP-9 from MCF-7 breast cancer cells. We found that many regulators of exocytosis, including several Rab GTPases, Rab effector proteins, and SNARE/SNARE modulator proteins, are stably assembled on docked secretory vesicles before exocytosis. At the moment of fusion, many of these components are quickly lost from the vesicle, while several endocytic proteins and lipids are simultaneously recruited to exocytic sites at precisely that moment. Our findings provide insight into the dynamic behavior of key core exocytic proteins, accessory proteins, lipids, and some endocytic proteins at single sites of secretory vesicle fusion in breast cancer cells.

Heterogeneity of endocytic proteins: distribution of clathrin adaptor proteins in neurons and glia

Neuroscience ◽  
2003 ◽  
Vol 121 (1) ◽  
pp. 25-37 ◽  
Author(s):  
P.J Yao ◽  
P Zhang ◽  
M.P Mattson ◽  
K Furukawa
Keyword(s):  
Adaptor Proteins ◽  
Clathrin Adaptor ◽  
Endocytic Proteins

scholarly journals P1-019: Upregulation of endocytic proteins in aged Tg2576 mice

2011 ◽  
Vol 7 ◽  
pp. S117-S117 ◽  
Author(s):  
Rhian S. Thomas ◽  
Emma Kidd ◽  
Mark Good ◽  
Mariah Lelos
Keyword(s):  
Tg2576 Mice ◽  
Endocytic Proteins

scholarly journals Phylogenetic Analysis of the NEEP21/Calcyon/P19 Family of Endocytic Proteins: Evidence for Functional Evolution in the Vertebrate CNS

2009 ◽  
Vol 69 (4) ◽  
pp. 319-332 ◽  
Author(s):  
Nagendran Muthusamy ◽  
Sanaa A. Ahmed ◽  
Brinda K. Rana ◽  
Sammy Navarre ◽  
David J. Kozlowski ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Functional Evolution ◽  
Endocytic Proteins

scholarly journals It's HIP to be a hub

2005 ◽  
Vol 170 (2) ◽  
pp. 169-171 ◽  
Author(s):  
Manuela Vecchi ◽  
Pier Paolo Di Fiore
Keyword(s):  
Network Theory ◽  
Transcriptional Activity ◽  
Hormone Receptors ◽  
Nuclear Hormone Receptors ◽  
Interacting Protein ◽  
Endocytic Protein ◽  
Huntingtin Interacting Protein 1 ◽  
Huntingtin Interacting Protein ◽  
Endocytic Proteins

Many endocytic proteins shuttle between the nucleus and the cytoplasm; however, their putative function in the nucleus is unclear. Now, new data demonstrate that huntingtin interacting protein 1 (HIP1), an endocytic protein, modulates the transcriptional activity of nuclear hormone receptors. In network theory, therefore, HIP1 can be regarded as a hub connecting heterogeneous functional “territories:” a possibility with important physiological and pathological implications.

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