scholarly journals Parasitised red blood cells misclassified as giant platelets by an automated digital morphology analyser (Sysmex DI‐60/CellaVision): a case report and a retrospective EQA analysis

2020 ◽  
Author(s):  
Marco Rosetti ◽  
Giorgia Farneti ◽  
Marta Monti ◽  
Arianna Torri ◽  
Giovanni Poletti ◽  
...  
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Giant Platelets ◽  
Digital Morphology

Glycophorin A-based exclusion of red blood cells for flow cytometric analysis of platelet glycoprotein expression in citrated whole blood

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Christina Berens ◽  
Johannes Oldenburg ◽  
Bernd Pötzsch ◽  
Jens Müller
Keyword(s):  
Red Blood Cells ◽  
Expression Analysis ◽  
Whole Blood ◽  
Blood Cells ◽  
Control Sample ◽  
Small Sample ◽  
Platelet Glycoprotein ◽  
Glycophorin A ◽  
Giant Platelets ◽  
Platelet Counts

AbstractObjectivesAnalysis of platelet glycoprotein (GP) expression by flow cytometry is applied for diagnostic confirmation of GP-associated thrombocytopathies. While platelet-rich plasma may be used for distinct identification of target events, this strategy is not feasible for small sample volumes or for patients showing low platelet counts and/or giant platelets. However, also the use of whole blood (WB) is hampered by the difficulty to discriminate platelets from red blood cells (RBC) in such patients. To circumvent these limitations, we evaluated the feasibility of a RBC gating-out strategy.MethodsIn addition to platelet GPIb, GPIIa/IIIa, as well as P-selectin (CD62P), citrated whole blood (CWB) samples were stained for RBC-specific glycophorin A (CD235a). CD235a-negative platelet events were further discriminated by forward-/side-scatter characteristics and platelet GP expressions analyzed relative to that of a healthy control sample processed in parallel.ResultsEstablished reference intervals allowed for clear identification of decreased GPIIb/IIIa- or GPIb expression pattern in samples of patients with confirmed Glanzmann thrombasthenia or Bernard–Soulier syndrome, respectively. It could be shown that the analysis of 2,500 platelet events is sufficient for reliable GP expression analysis, rendering the proposed method applicable to samples with low platelet counts.ConclusionsThis study demonstrates the feasibility of CD235a-based exclusion of RBC for platelet GP expression analysis in CWB. In contrast to direct staining of platelet-specific antigens for target identification, this indirect gating out approach is generally applicable independent of any underlying platelet GP expression deficiency.

Transfusion reaction due toKlebsiella pneumoniae-contaminated red blood cells: a case report

2013 ◽  
Vol 23 (6) ◽  
pp. 445-446 ◽  
Author(s):  
P. Warnke ◽  
V. Kiefel ◽  
H. Schäffler ◽  
A. Podbielski
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Transfusion Reaction

scholarly journals Adverse reaction after transfusion of Red Blood Cells in a patient aged 97 years - case report

2021 ◽  
Vol 11 (9) ◽  
pp. 96-100
Author(s):  
Paulina Marta Kasperska-Dębowska ◽  
Eliza Oleksy ◽  
Anna Wojtczak ◽  
Małgorzata Nowicka
Keyword(s):  
Adverse Reaction ◽  
Case Report ◽  
Blood Transfusion ◽  
Older People ◽  
Red Blood Cells ◽  
Cardiovascular System ◽  
General Condition ◽  
Blood Cells ◽  
Stable State

During hospitalization 97 years old patient had blood transfusion. This procedure lead to overload of cardiovascular system. Pharmacotherapy gradually improved the general condition of the patient. The patient was discharged in a stable state. Doubts arise; when blood should be transfused, in which cases should a blood transfusion be discontinued, what should be the recommendation for a blood transfusion for older people over the age of 90. There is a need for further research in geriatrics regarding the transfusion of blood and its components.

Alteration of human red blood cells stored in plastic packs. A case report with in vivo and in vitro studies

Transfusion ◽  
1982 ◽  
Vol 22 (2) ◽  
pp. 154-157 ◽  
Author(s):  
JT Callahan ◽  
MF Collecutt ◽  
JR Lightbody ◽  
BS Faragher
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Studies ◽  
Human Red Blood Cells

Red cell exchange employing phenotypically matched deglycerolized red blood cells to treat acute sickle cell crisis: A case report

2009 ◽  
Vol 41 (2) ◽  
pp. 155-156
Author(s):  
Cesare Perotti ◽  
Paola Isernia ◽  
Claudia Del Fante ◽  
Gianluca Viarengo ◽  
Laura Salvaneschi ◽  
...  
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Red Cell ◽  
Sickle Cell Crisis

Pseudohyperkalemia identified in a myelofibrosis patient exhibiting giant platelets and nucleated red blood cells

2007 ◽  
Vol 68 (07) ◽  
pp. 57-59 ◽  
Author(s):  
C. Duplessis ◽  
D. Rakowski ◽  
E. Yeung ◽  
M. Hopkins
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Giant Platelets ◽  
Nucleated Red Blood Cells
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